The invention provides a chemiluminiscence immunoassay kit for detecting zilpaterol, and belongs to the field of immunological detection. The kit comprises a non-transparent white ELISA plate coated with a zilpaterol-carrier protein conjugate, a zilpaterol standard substance, a zilpaterol-peroxidase labeled antibody working solution, a luminescent substrate solution, a concentrated sample diluent and a concentrated washing liquid. The zilpaterol-carrier protein conjugate is obtained by coupling zilpaterol and a carrier protein through a mixed anhydride method or a carbodiimide method, and the concentrated washing liquid contains 0.05% of Twain-20. Compared with a traditional enzyme-linked immunosorbent assay method, the kit has higher sensitivity degree, is short in detection time and low in detection cost, and can be used for detection of the residual amount of zilpaterol in animal urine, blood samples, tissues, viscera and other samples.