Preparation method of chemiluminescence immunoassay detection kit used for detecting methyl parathion

Abstract

The invention provides a chemiluminescence immunoassay detection kit used for detecting methyl parathion (MP), and belongs to the field of immunological detection. The chemiluminescence immunoassay detection kit is composed of a non-transparent white enzyme label plate coated with a methyl parathion (MP)-carrier protein conjugate, a methyl parathion standard substance, a methyl parathion-peroxidase labelled antibody working liquid, a luminescent substrate liquid, a concentrated sample diluent, and a concentrated washing liquid. The methyl parathion (MP)-carrier protein conjugate is obtained via coupling of methyl parathion with a carrier protein via mixed anhydride method or carbodiimide method; and the concentrated washing liquid contains 0.05% of tween-20. The chemiluminescence immunoassay detection kit is high in sensitivity; detection time is short; cost is low; and the chemiluminescence immunoassay detection kit can be used for residual amount detection of methyl parathion (MP) in samples such as fruits, vegetables, and crops.

Description

Technical field [0001] The invention relates to a chemiluminescence immunoassay kit for detecting methyl parathion (MP), which is used for detecting the content or residual amount of methyl parathion (MP) in fruits, vegetables, crops and the like. It belongs to the field of immunological detection. Background technique [0002] Parathionmethyl (Parathionmethyl), also called O,O-dimethyl-O-p-nitrophenyl thiophosphate, is a highly toxic organophosphate insecticide with large output and wide application. It is highly toxic and has been banned on vegetables and fruits in recent years. However, in vegetable production, there are still the phenomenon of misuse and abuse of pesticides. In the supervision and testing of import and export trade of agricultural products and food safety, methyl parathion (MP) is still the focus of routine testing. [0003] Methyl parathion (MP) residue analysis generally uses gas chromatography (GC), high performance liquid chromatography (HPLC) and gas chr...

AI Technical Summary

Problems solved by technology

[0003] Methyl parathion (MP) residue analysis generally uses gas chromatography (GC), high performance liquid chromatography (HPLC) and gas chromatography-mass spectrometry (GC / MS). These methods are sensitive and accurate, and can simultaneously determine multiple It is a kind of drug, but it needs expensive instruments, the sample pretreatment is complex, tedious and time-consuming, the detection cost is high, and it needs to be operated by professionals, it is difficult to meet the needs of on-site, batch and rapid detection of samples