Chemiluminesent immunoassay kit for detection of bisphenol A

A chemiluminescence immunoassay, detection kit technology, applied in biological testing, measuring devices, scientific instruments, etc., can solve the problems of expensive detection instruments, high detection costs, low detection positive rate, etc., and achieve low detection costs and increased sensitivity. , the effect of simple instrument

Inactive Publication Date: 2014-05-14
JIANGSU WISE SCI & TECH DEV
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AI Technical Summary

Problems solved by technology

[0003] There are many analysis and testing methods for bisphenol A. At present, there are mainly spectrophotometry and fluorescence assay, capillary electrophoresis, gas chromatography and gas-mass spectrometry, liquid chromatography and liquid-mass spectrometry, etc. These methods are accurate in detection. High precision, low positive rate, etc., but the detection equipment is expensive, the operation is cumbersome, time-consuming, and the detection cost is high

Method used

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Experimental program
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Embodiment 1

[0038] 1. Preparation of each component of the kit

[0039] (1) Preparation of bisphenol A hapten: bisphenol A was acidified, and reacted with sodium nitrite in a dark and low-temperature environment at 4°C to generate an intermediate containing diazonium cations. The diazotized bisphenol A is used as a hapten for subsequent synthesis of immune antigens and coating antigens;

[0040] (2) Preparation of bisphenol A-bovine serum albumin (BSA) immunogen: bisphenol A and bovine serum albumin (BSA) are coupled by diazotization method to obtain immune antigen;

[0041] (3) Preparation of bisphenol A-oval serum albumin (OVA) coated antigen: bisphenol A and egg serum albumin (OVA) were coupled by diazotization method to obtain coated antigen;

[0042](4) Preparation of bisphenol A-peroxidase-labeled antibody: For 6-8 week-old female BALB / c mice (body weight 18-20 g), the high-dose immunization scheme is as follows: 160 μg bisphenol A for the first immunization A-BSA was mixed with a...

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Abstract

Belonging to the technical field of industrial compound environmental hormone detection, the invention provides a chemiluminesent immunoassay kit for detection of bisphenol A. The kit provided by the invention is composed of a non-transparent white enzyme-labeled plate coated with a bisphenol A-carrier protein conjugate, a bisphenol A standard substance, a bisphenol A-peroxidase labeled antibody working solution, a luminescent substrate solution, a concentrated sample dilution solution, and a concentrated washing solution. The bisphenol A-carrier protein conjugate is obtained by coupling bisphenol A and carrier protein through a mixed anhydride method or a carbodiimide method, and the concentrated washing solution contains 0.05% Tween-20. Compared with the traditional enzyme linked immunosorbent assay method, the kit provided by the invention has the advantages of higher sensitivity, short detection time and low cost, and can be used for detection of residual bisphenol A in an environmental water sample.

Description

technical field [0001] The invention relates to a chemiluminescent immunoassay kit for detecting bisphenol A, which is used for detecting the content or residual amount of bisphenol A in environmental water samples. The invention belongs to the technical field of detection of industrial compound environmental hormones. Background technique [0002] Bisphenol A (BPA), the raw material for polycarbonate, epoxy and phenolic resins, is one of the most widely used industrial compounds in the world. Widely used in the coating of food packaging and container interiors, such as canned food and beverage packaging, baby bottles, water bottles, sealants for dental fillings, eyeglass lenses and hundreds of other everyday products. BPA enters the water environment through the atmosphere or sewage during production and use, and then gradually decomposes under the action of microorganisms, with a half-life of about 2.5-4.0 days. BPA is an endocrine disruptor. Studies in recent years hav...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/74
CPCG01N33/54373G01N33/74
Inventor 杜道林洪霞
Owner JIANGSU WISE SCI & TECH DEV
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