Preparation of compound glass nanopore and application of compound glass nanopore to biomolecular detection

A technology of nano-channels and composite glass, which is applied in nano-drugs, nano-technology, nano-technology and other directions, can solve the problems of unfavorable popularization, complicated detection instruments and high cost, and achieves the effect of simple method, universality, and favorable promotion and use.

Active Publication Date: 2019-07-19
WENZHOU UNIVERSITY
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Problems solved by technology

The detection methods at the present stage are all for the detection of a single biomolecule, and all of them require the use of expensive reagent materials such as fluorescent markers or biological kits. The cost is high and the required detection instruments are complicated, which is not conducive to popularization

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  • Preparation of compound glass nanopore and application of compound glass nanopore to biomolecular detection
  • Preparation of compound glass nanopore and application of compound glass nanopore to biomolecular detection
  • Preparation of compound glass nanopore and application of compound glass nanopore to biomolecular detection

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Embodiment Construction

[0036] In order to make the object, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings.

[0037] Step 1: Prepare glass nanopores; the operation method is as follows:

[0038] ①Take a glass tube whose specification is outer diameter: 1.2mm; inner diameter: 0.6mm; length: 10mm;

[0039] ② Place the glass tube in concentrated sulfuric acid (H 2 SO 4 ), hydrogen peroxide (H 2 o 2 ) mixed solution, ultrasonic for about 15min. Rinse with high-purity water and ethanol, and then place it in high-purity water for later use;

[0040]③Clamp out the cleaned glass tube with tweezers, rinse it with ethanol, and dry it with nitrogen, and set it aside;

[0041] ④Then draw the glass tube into a glass nanochannel with a suitable pore size on a glass tube drawing machine with certain parameters (the parameters are shown in Table 1 above). The nanopore size obtained...

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Abstract

The invention discloses a preparation method of a compound glass nanopore and application of the compound glass nanopore to biomolecular detection. The preparation method comprises the following specific operation steps of 1, preparing a glass nanopore; 2, modifying phospholipids on the glass nanopore prepared in the step 1; 3, preparing the compound glass nanopore; and 4, measuring current data between electrodes with the glass nanopore modified with the phospholipids provided with SWCNT in an inserted manner through a picoammeter and a patch clamp and recording the current data. According tothe preparation method, the glass nanopore is pulled by utilizing a glass puller, and the phospholipids provided with the SWCNT in an inserted manner are modified in the glass nanopore through the capillary phenomenon of nanopores. The operations of ultrasonically cutting the long-time purified SWCNT and utilizing a microinjection probe to insert the SWCNT into bimolecular layers of the phospholipids are not required, so that the method is simple. The current signal changes of different biomacromolecules can be measured through a specific pore of the compound glass nanopore, so that the compound glass nanopore is suitable for detecting the other different target molecules, has universality and is beneficial for application and popularization.

Description

technical field [0001] The invention relates to the field of nano-channel detection, in particular to a preparation method of a glass nano-channel composite glass nano-channel based on single-armed carbon nanotube (SWCNT) and phospholipid self-assembly and its application in biomolecular detection. Background technique [0002] As a new type of analysis and detection method, nanopore technology is widely used in nucleic acid sequencing, protein / peptide analysis, and detection of biological macromolecules and metal ions such as viruses and microorganisms. As people pay more and more attention to issues such as public safety and food and drug safety, higher requirements are put forward for the detection of toxic substances. In view of the advantages of high sensitivity and high selectivity of the nanopore analysis method, many research teams have applied it to the detection of toxic substances and conducted a lot of research work. The current detection methods are all for the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/28G01N33/487B82Y40/00B82Y5/00
CPCB82Y5/00B82Y40/00G01N27/28G01N33/48721
Inventor 刘楠楠叶婷艳唐星星董幼青
Owner WENZHOU UNIVERSITY
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