Urinary microalbumin (U-mALb)/urinary creatinine (U-Cr) integrated assay bigeminy strip and preparation method thereof

Abstract

The invention relates to a urinary microalbumin (U-mALb) / urinary creatinine (U-Cr) integrated assay bigeminy strip, comprising a sample area and reaction areas. The U-mALb / U-Cr integrated assay bigeminy strip is characterized in that the sample area comprises a diffusion membrane and blood filtering membranes; the reaction areas comprise two detection windows, and the two detection windows can be used for detecting the ratios of the U-mALb and the U-Cr at the same time; the sample area for detecting the U-Cr by adopting a dry chemical method is arranged at the position where the reaction area is positioned, and the reaction area for detecting the U-mALb by using a colloidal gold dry type immune chromatography technology is arranged at a position which is 0.5cm away from the sample area. The different reaction areas contain different reagent pads, and the reagent pads respectively and independently react with corresponding components in urine. Synchronous detection can be realized only by dripping the random urine of a person to be tested into the sample area, so that the U-mALb / U-Cr integrated assay bigeminy strip is very suitable for medical institutions or individuals. By using the U-Cr for correction, the influence, caused by a sampling way and urine volume, on the excretory amount of urinary albumin can be eliminated, so that the U-mALb / U-Cr integrated assay bigeminy strip can provide more accurate evaluation for the albumin excretion rate and has a greater clinical significance for preventing diabetic nephropathy.

Description

technical field [0001] The invention relates to a urine microalbumin / urine creatinine integrated detection dual strip, which belongs to the technical field of clinical in vitro diagnosis. Background technique [0002] 1. In recent years, with the increase of life expectancy in my country, changes in living habits and diet results have led to an obvious upward trend in the prevalence of diabetes. Diabetic nephropathy (DN) is one of the most serious complications of diabetes, and it is also an end-stage disease. It is one of the main causes of kidney disease. Therefore, for diabetic patients, it is not only necessary to check blood sugar frequently to monitor the condition, but also need to monitor the condition of their kidneys through microuria protein and urine creatinine; [0003] 2. Due to the long-term high blood sugar and the decrease of insulin in diabetic patients, the renal microvessels are damaged, and the filtering function of the glomeruli is greatly reduced, resul...

AI Technical Summary

Problems solved by technology

This method is very inconvenient for patients, and the cost of the experiment is also high. There is no uniform, simple and low-cost method for the early screening of diabetes and hypertensive nephropathy in China

At present, there is no integrated detection reagent strip that combines dry biochemical and dry immunochromatography technologies in the market, and the market application prospect in this direction is huge;

[0005] 4. At present, there are few products on the market that can detect urine microalbumin (U-mALb) and urine creatinine (U-Cr), and most of them are imported products

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