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Quantitative testing reagent for liquid microalbumin in urine and method

A technology for the detection of urine microalbumin and quantitative detection method, applied in the biological field, can solve the problems of false positives, many factors affected by dry chemical test strips, and inability to quantify, and achieve the effect of rapid operation, accurate and reliable diagnosis information

Inactive Publication Date: 2012-09-19
JIAXING JIUQIJIU BIOLOGICAL TECH
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AI Technical Summary

Problems solved by technology

The urine test technology currently used in China is mainly the qualitative test system of dry chemical test paper, which can only provide qualitative analysis results such as negative or positive, and cannot be quantitative
At the same time, there are many factors affected by the dry chemical test paper method, and false negatives and false positives will inevitably occur in clinical work.
In addition, dry chemical test strips are limited by their sensitivity, and they are only positive when they are greater than 20 mg / dL, and at this time the kidney disease is irreversible

Method used

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  • Quantitative testing reagent for liquid microalbumin in urine and method
  • Quantitative testing reagent for liquid microalbumin in urine and method

Examples

Experimental program
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Effect test

Embodiment 1

[0026] Embodiment 1: the preparation steps of a kind of urinary microalbumin liquid quantitative test reagent:

[0027] a. Add the following chemicals to the beaker: 4 g sodium chloride, 5.5 g disodium hydrogen phosphate, 1.0 g potassium dihydrogen phosphate, 5 g sodium azide, 10 g polyethylene glycol, 1 L distilled water, stir until solid chemical The substance is completely dissolved to form R1 reagent;

[0028] b. Dissolve 0.5 g of sodium azide in 1 L of PBS buffer, stir until completely dissolved, and form an intermediate solution;

[0029] c. Add anti-human albumin antibody with a volume ratio of 1:50 to the intermediate solution to form a homogeneous R2 reagent.

[0030] Example 2 3 4 5 6 7 8 9 10 11 12 13 Sodium chloride (g) 2 10 10 8 4 4 6 6 8 8 2 10 Disodium hydrogen phosphate (g) 1 1 3.5 3.5 4.5 4.5 5.5 5.5 7.5 7.5 10 10 Potassium dihydrogen phosphate (L) 0.1 0.1 0.5 0.5 1.0 1...

Embodiment 14

[0031] Embodiment 14: A quantitative detection method of urinary microalbumin liquid, the quantitative detection method of urinary microalbumin liquid according to the present invention is based on the principle of immunoturbidimetric method, the microalbumin in urine and the antibody in the reagent Human albumin antibody forms an immune complex, which has an absorption peak at a wavelength of 340nm, and the concentration of albumin can be determined by the amount of incident light absorbed. The absorbance value is proportional to the concentration of albumin, calibrators and controls in the sample.

[0032] The urine microalbumin liquid quantitative detection method of any reagent of the present invention comprises the following steps:

[0033] a. Put the R1 reagent and R2 reagent of the urine microalbumin liquid quantitative detection reagent into the R1 and R2 reagent compartments of the automatic analyzer respectively after subpackaging, and then perform the parameter set...

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Abstract

The invention discloses a quantitative testing reagent for liquid microalbumin in urine and a method. The quantitative testing reagent for the liquid microalbumin in urine consists of an R1 reagent and an R2 reagent, wherein the R1 reagent is obtained by dissolving a certain amount of sodium chloride, sodium hydrogen phosphate, potassium dihydrogen phosphate, sodium azide and polyethylene glycol in distilled water; and the R2 reagent is obtained by dissolving a certain amount of sodium azide in phosphate buffer solution and then adding a certain amount of anti-human albumin antibody into the mixture. The self-developed testing reagent for the liquid microalbumin in urine is combined with an automatic analyzer system to precisely determine the content of microalbumin in urine, so as to provide accurate information for a clinician and possibly test the urine sample quantitatively, automatically and fast.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a reagent and method for quantitatively detecting urine microalbumin (Urinary Microalbumin) liquid. Background technique [0002] Urine is formed by the filtration of certain blood components by the glomeruli, reabsorption, collection, and excretion by the renal tubules. Clinical urinalysis can directly help doctors understand the changes in the patient's urinary physiological and pathological functions, and can also indirectly reflect the function of multiple organs and systems throughout the body. Clinical urine tests are very common at home and abroad, and many tests related to renal function, cardiovascular, metabolic diseases, infectious diseases, etc. need to be carried out with urine. Routine urine tests include the following 10 items: glucose (GLU), bilirubin (BIL), urobilinogen (URO), ketone bodies (KET), leukocytes (LEC), nitrite (NIT), protein (PRO) , pH (pH), blood occult...

Claims

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Application Information

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IPC IPC(8): G01N33/68
Inventor 虞留明蔡江丽田军孟雷
Owner JIAXING JIUQIJIU BIOLOGICAL TECH
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