The invention relates to the field of anti-autologous AchR
antibody detection equipment, in particular to a method for efficiently detecting an anti-autologous AchR
antibody, which comprises the following operation steps: S1,
antigen coating: selecting a proper
microwell plate, diluting an AchR
antigen to a proper concentration by using a
carbonate buffer solution, adding 100-200 [mu] L of an
antigen solution into each well, then placing the
microwell plate in a refrigerator at 4 DEG C overnight, and taking the
microwell plate out of the refrigerator; the antigen can be fully adsorbed on the surface of the microwell plate; s2, closing the microwell plate: after overnight
coating, pouring away the antigen solution in the microwell plate, washing the microwell plate with a washing solution for three times, soaking for 3-5 minutes each time to remove uncombined antigens, then adding 200-300 microliters of a sealing solution into each hole, incubating for 1-2 hours at 37 DEG C, and after incubation is finished; according to the invention, the anti-autologous AchR
antibody is detected based on the highly specific binding reaction of the antigen and the antibody, and the layer-by-layer specific binding mechanism enables the ELISA to have high specificity on the detection of the target antibody, so that the interference caused by non-specific binding can be effectively reduced.