Kit and method for quantitatively detecting urine microalbumin through time-resolved fluorescence

Abstract

The invention relates to a kit and a method for quantitatively detecting urine microalbumin through time-resolved fluorescence. The kit comprises a time-resolved fluorescence test paper card, sample diluent and a DS card containing a calibration curve, wherein the time-resolved fluorescence test paper card comprises a detection test paper strip which consists of a bottom liner, and a sample pad, a nitrocellulose membrane and absorbent paper which are pasted on the bottom liner; the nitrocellulose membrane is successively provided with a microsphere line, a detection line and a quality control line; the microsphere line consists of time-resolved fluorescence microspheres marked with human seroalbumin monoclonal antibodies; the detection line is coated with human seroalbumin recombinant antigens; the quality control line is coated with goat anti-mouse IgG. The kit provided by the invention can accurately and quantitatively detect the content of microalbumin in human urine; markers are used for connecting the microspheres with antibodies through covalent bonds; and the marked products are stable. The kit has the characteristics of wide detection range (2 to 300mg / ml), high sensitivity (the detection limit is 2300mg / ml), high accuracy, fast and easy detection and the like.

Description

[0001] (1) Technical field [0002] The invention relates to a time-resolved fluorescent quantitative detection kit and detection method for urine microalbumin. [0003] (2) Background technology [0004] Urine microalbumin refers to the presence of microalbumin in urine. Albumin is a normal protein in the blood. Under normal human metabolism, there is very little albumin in urine, and albumin per liter of urine does not exceed 20mg ( <20mg / L), so it is called microalbumin. However, early kidney damage such as diabetic nephropathy and hypertensive nephropathy and complications of cardiovascular disease can cause damage to the kidney's inherent cell structure and function, resulting in increased albumin content in the urine. If the microalbumin in the urine is in the range of 20~200mg / L, it belongs to microalbuminuria. When the microalbumin in the urine exceeds 200mg / L, it proves that the patient has a large amount of albumin leakage and hypoalbuminemia may occur. It can develop ...

AI Technical Summary

Problems solved by technology

Among them, the colloidal gold method has the advantages of simple and fast operation, but its sensitivity is low and its quantification is inaccurate; the enzyme-linked immunoassay method has high sensitivity and large sample volume can be quantitatively detected, but the operation time is long and the automation is low; immunoturbidimetric method and chemiluminescence method are relatively Sensitive and accurate, it can be applied to automatic biochemical analyzers, but requires instruments and takes a long time, is suitable for processing a large number of samples, and cannot meet the purpose of rapid detection; immunofluorescence method is to covalently bind urine microalbumin antibodies to fluorescent microspheres On the surface active group, the result is judged by whether the detection line generates fluorescence after excitation. It is fast, convenient, accurate and quantitative, and has the advantages of high sensitivity and stable markers. It is widely used in the field of medical immunoassay

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