9results about How to "Avoid purification process" patented technology

The invention belongs to the technical field of tumor vaccines, and discloses a personalized tumor vaccine based on hydrogel and a preparation method and application thereof, the preparation method comprises the following steps: adding a chemotherapeutic drug into a mixed solution of polyphenol and metal ions, uniformly mixing, adjusting the pH value of the solution, and reacting for 2-8 hours to obtain a metal polyphenol nano-carrier loaded with the chemotherapeutic drug; co-culturing the obtained metal polyphenol nano-carrier loaded with the chemotherapeutic drug and tumor cells for 12-48 hours, and inducing the tumor cells to generate immunogenic cell death, wherein the concentration of the metal polyphenol nano-carrier loaded with the chemotherapeutic drug is 1-20 [mu] g / mL; the tumor cell suspension in the ICD state and the STING agonist solution are mixed with the sodium alginate solution, and the personalized tumor vaccine is obtained. The ICD is induced in vitro by the chemotherapeutic drug, so that the toxic and side effects of systemic administration are reduced, the types of antigens are enriched, and a powerful guarantee is provided for the vaccine to activate a strong in-vivo anti-tumor immune response.

The invention discloses a composite hydrogel, a composite aerogel, a preparation method and an application. The preparation method of the composite hydrogel comprises the following steps: irradiating a mixed solution A with high-energy rays; wherein the mixed solution A comprises graphene oxide, an inorganic nano material, monomers of acrylic acid and derivatives thereof, water and an alcohol water-soluble compound; the inorganic nano material is selected from one or more of an MXene two-dimensional lamellar material, a metal oxide and a metal sulfide; in the mixed solution A, the content of the graphene oxide is 0.05-10 mg / mL, the mass ratio of the graphene oxide to monomers of acrylic acid and derivatives thereof is 1: (50-1000), and the mass ratio of the graphene oxide to the inorganic nano-material is 1: (0.1-100); the alcohol water-soluble compound accounts for 2-50% of the mixed solution A in percentage by mass. When the composite hydrogel and the composite aerogel are applied to the field of solar photo-thermal water evaporation, an evaporator is not prone to being blocked.

The present invention belongs to the technical field of tumor vaccines, and discloses a personalized tumor vaccine based on hydrogel, and a preparation method and application thereof, wherein the preparation method comprises the following steps: adding a chemotherapeutic drug to a mixed solution of polyphenols and metal ions, mixing, adjusting the pH value of the solution, reacting for 2-8 hours, and obtaining a metal polyphenol nanocarrier loaded with a chemotherapeutic drug; co-culturing the obtained metal polyphenol nanocarrier loaded with a chemotherapeutic drug with tumor cells for 12-48 hours, wherein the concentration of the metal polyphenol nanocarrier loaded with a chemotherapeutic drug is 1-20 μg / mL, inducing immunogenic cell death in tumor cells; mixing a tumor cell suspension in an ICD state and a STING agonist solution with a sodium alginate solution, and obtaining a personalized tumor vaccine. The chemotherapeutic drug induces ICD in vitro, which not only reduces the toxic and side effects of systemic administration, but also enriches the types of antigens, providing a strong guarantee for the vaccine to activate a powerful anti-tumor immune response in vivo.

The invention discloses an engineering strain for producing urolithin A and application of the engineering strain. The engineering strain contains a tannin acyl hydrolase gene tah, an esterase gene lip, a gallic acid decarboxylase gene gdo and a dopamine dehydroxylase gene ascd. The four artificially synthesized genes are jointly constructed on an escherichia coli expression vector, so that a brand-new vector containing four co-expression genes is constructed, then the brand-new vector is transferred into a chassis strain cell to realize co-expression of the four genes, and four enzymes expressed in the cell all have activity; and the obtained heavy engineering strain can stably convert the extracellular added ellagic tannin into the urolithin A, so that the whole-cell catalysis process of producing the urolithin A by taking the ellagic tannin as a substrate is realized.

The invention relates to extraction of sialylated glycocalyxin, in particular to a serotonin-silicon dioxide microsphere compound, a preparation method thereof and a sialylated glycocalyxin extraction process, and aims to solve the problems that in the prior art, when the serotonin is used as a ligand to enrich the sialylated glycocalyxin, the sialylated glycocalyxin is difficult to obtain through rapid separation, and the purity of the sialylated glycocalyxin is low. Meanwhile, when the serotonin-magnetic particle compound is used for enriching the sialylated glycocalyxin, impurities difficult to remove are easily generated, and the method repeatability is poor. According to the extraction process of the sialylated glycocalyxin, a serotonin-silicon dioxide microsphere compound and the sialylated glycocalyxin are combined and then eluted, and the serotonin-silicon dioxide microsphere compound uses silicon dioxide microspheres as a carrier; the serotonin is covalently coupled to the surface of the epoxidized / activated carboxylation / formylation modified silicon dioxide microsphere, the combination and elution time is short, the separation efficiency is high, and the method is suitable for industrial production.

The invention relates to the field of organic synthesis, in particular to a method for preparing Boc-His (Trt)-Aib-OH, which comprises the following steps: 1, adding 2-aminoisobutyric acid, a solvent and organic alkali into a reaction container, cooling, dropwise adding a silicon protection reagent, and heating for reaction after adding to obtain a 2-aminoisobutyric acid solution protected by bis (trialkylsilyl); 2, dissolving Boc-His (Trt)-OH and an activating agent in a solvent, dropwise adding a coupling reagent, and after the reaction is finished, obtaining a Boc-His (Trt)-OH activated ester solution which is directly used for the next step; and step 3, cooling the Boc-His (Trt)-OH activated ester solution obtained in the step 2, dropwise adding the bis-trialkylsilyl protected 2-aminoisobutyric acid solution obtained in the step 1, dropwise adding organic alkali after the dropwise adding is completed, slowly dropwise adding a quenching reagent for quenching after the reaction is completed, carrying out post-treatment on the reaction solution to obtain a Boc-His (Trt)-Aib-OH crude product, and drying to obtain the Boc-His (Trt)-Aib-OH. And crystallizing the crude product to obtain a pure product of Boc-His (Trt)-Aib-OH. According to the method, purification of an intermediate is avoided, the reaction process is relatively well controlled, and high-quality Fmoc-Ser (tBu)-OH can be efficiently and stably prepared.

The invention discloses a comprehensive treatment system for foul gas and carbon dioxide in a livestock farm. The comprehensive treatment system comprises an ammonia gas collecting chamber, an ammonia water collecting device and a reaction kettle which are communicated with the livestock farm. A negative pressure device for forming negative pressure is arranged in the ammonia gas collecting chamber, so that waste gas of the farm is sucked into the ammonia gas collecting chamber, an ultra-micro bubble generator is arranged at the top of the ammonia gas collecting chamber and is used for forming ionized ultra-micro bubbles, and a drainage water tank is arranged at the bottom of the ammonia gas collecting chamber and is used for draining the waste gas in the farm. Ammonia gas and carbon dioxide in the waste gas of the livestock farm are dissolved in the ultramicro bubbles and fall into the drainage water tank, the drainage water tank guides the collected mixed solution to the ammonia water collecting device, and the ammonia water collecting device collects the mixed solution and sends the mixed solution to the reaction kettle; after the mixed solution reacts with carbon dioxide and brine in the reaction kettle, byproducts such as ammonium salt and bicarbonate are obtained through solid-liquid separation, additional economic value is generated, carbon dioxide in a field area is neutralized, and the air quality of the farm is improved.

The present invention relates to the technical field of recombinant protein design and synthesis, and specifically discloses a recombinant spider silk protein and its synthesis method. The recombinant spider silk protein provided by the present invention undergoes optimized design such as replacement of the N-terminal and C-terminal domains of different spider species and different spider silk types, and large fragment recombination of the middle functional domain repeat sequences. A series of recombinant spider silk proteins are obtained through cell-free protein synthesis methods and prokaryotic fermentation expression. Compared with natural spider dragline silk proteins or other existing recombinant spider silk proteins, they generally have good composite mechanical properties and high-temperature resistance characteristics. At the same time, they have a relatively small molecular weight, significantly improving the total expression level and soluble expression, providing new raw materials and design and synthesis methods for the development of high-performance biomaterials.

The application provides a synthesis method of 3-(5-fluoropyrimidin-2-yl)-2-methoxy aniline, and belongs to the technical field of chemical synthesis. The application first provides an intermediate for synthesizing 3-(5-fluoropyrimidin-2-yl)-2-methoxy aniline shown in formula I and formula II. The intermediate can be used to successfully prepare 3-(5-fluoropyrimidin-2-yl)-2-methoxy aniline. The synthesis method is simple in operation, high in product yield and purity. Meanwhile, in the key C-C bond coupling, a low-cost metal Ni catalyzed reductive coupling strategy is used to replace the traditional two-step Pd catalyzed coupling reaction, so that the material cost of the reaction is greatly reduced, and the problem of regioselectivity in the step of introducing a nitro group is avoided, and further purification is avoided. The synthesis method is simple and efficient, low in cost, and has a good industrialization prospect.