3results about How to "Avoid purification process" patented technology

The invention discloses an engineering strain for producing urolithin A and application of the engineering strain. The engineering strain contains a tannin acyl hydrolase gene tah, an esterase gene lip, a gallic acid decarboxylase gene gdo and a dopamine dehydroxylase gene ascd. The four artificially synthesized genes are jointly constructed on an escherichia coli expression vector, so that a brand-new vector containing four co-expression genes is constructed, then the brand-new vector is transferred into a chassis strain cell to realize co-expression of the four genes, and four enzymes expressed in the cell all have activity; and the obtained heavy engineering strain can stably convert the extracellular added ellagic tannin into the urolithin A, so that the whole-cell catalysis process of producing the urolithin A by taking the ellagic tannin as a substrate is realized.

The present invention relates to the technical field of recombinant protein design and synthesis, and specifically discloses a recombinant spider silk protein and its synthesis method. The recombinant spider silk protein provided by the present invention undergoes optimized design such as replacement of the N-terminal and C-terminal domains of different spider species and different spider silk types, and large fragment recombination of the middle functional domain repeat sequences. A series of recombinant spider silk proteins are obtained through cell-free protein synthesis methods and prokaryotic fermentation expression. Compared with natural spider dragline silk proteins or other existing recombinant spider silk proteins, they generally have good composite mechanical properties and high-temperature resistance characteristics. At the same time, they have a relatively small molecular weight, significantly improving the total expression level and soluble expression, providing new raw materials and design and synthesis methods for the development of high-performance biomaterials.

The application provides a synthesis method of 3-(5-fluoropyrimidin-2-yl)-2-methoxy aniline, and belongs to the technical field of chemical synthesis. The application first provides an intermediate for synthesizing 3-(5-fluoropyrimidin-2-yl)-2-methoxy aniline shown in formula I and formula II. The intermediate can be used to successfully prepare 3-(5-fluoropyrimidin-2-yl)-2-methoxy aniline. The synthesis method is simple in operation, high in product yield and purity. Meanwhile, in the key C-C bond coupling, a low-cost metal Ni catalyzed reductive coupling strategy is used to replace the traditional two-step Pd catalyzed coupling reaction, so that the material cost of the reaction is greatly reduced, and the problem of regioselectivity in the step of introducing a nitro group is avoided, and further purification is avoided. The synthesis method is simple and efficient, low in cost, and has a good industrialization prospect.