Atrophic gastritis rare earth detection test strip, detection card, kit and preparation method

Abstract

The invention discloses an atrophic gastritis rare earth detection test strip, a detection card, a kit and a preparation method. The atrophic gastritis rare earth detection test strip comprises a bottom lining, and a sample pad, a combination pad, a nitrocellulose membrane and absorbent paper which are sequentially lapped on the bottom lining along the length direction. Rare earth nano fluorescentmicrospheres marked with PG I, PG II and rabbit IgG antibodies are sprayed on the combination pad. Monoclonal antibodies corresponding to PGI, PGII and rabbit IgG antibodies are sequentially marked on the nitrocellulose membrane to respectively serve as a first detection line, a second detection line and a quality control line. The first detection line is close to the combination pad, and the quality control line is close to the absorbent paper. The test strip has the characteristics of wide detection range, high sensitivity, high accuracy, rapidness, simplicity and convenience in detection and the like, and the detection result can help doctors to accurately diagnose atrophic gastritis in the early stage.

Description

technical field [0001] The invention relates to a rare earth detection test strip for atrophic gastritis, a detection card, a kit and a preparation method. Background technique [0002] Pepsinogen (PG) is composed of 375 amino acids and has a molecular weight of about 42k Da. It is mainly synthesized and secreted by gastric mucosal tissue, and can be activated into pepsin with digestive function under the stimulation of gastric acid. The sources of PGⅠ and PGⅡ are different. PGⅠ is mainly secreted by the principal cells and mucus neck cells of the fundic glands; while PGⅡ is derived from almost all gastric gland cells, such as gastric cardia glands, gastric fundic glands, gastric antrum pyloric gland cells, and even distal gastric gland cells. Terminal duodenum Brunner gland cells. Under normal circumstances, most of the synthesized PG enters the gastric cavity directly, and only a small amount (about 1%) enters the blood circulation through the gastric mucosal capillaries ...

AI Technical Summary

Problems solved by technology

For example, time-resolved fluorescent microspheres synthesized by swelling with rare earth complexes have disadvantages such as easy leakage of rare earth complexes and photobleaching; although the dissociation-enhanced method (DELFLA) has high sensitivity, it must add an enhancement solution to detect fluorescence; some fluorescence Microspheres have disadvantages such as weak fluorescence intensity

Images