Detection method, system and kit of microalbuminuria
A urine microalbumin and solution technology, applied in chemical instruments and methods, fluorescence/phosphorescence, organic dyes, etc., can solve the problems of polluting the environment, difficult to promote, and affecting the health of operators.
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Embodiment 1
[0065] In this example, the urine microalbumin concentration of three urine samples is verified. The actual urine microalbumin concentration of each urine sample is as follows: urine sample 1 is 5.84 mg / L, urine sample 2 is 22.25 mg / L, urine sample 2 is 22.25 mg / L, and urine sample 2 is 22.25 mg / L. Sample 3 was 68.17mg / L. The cyanine dye used in this example is a compound of the following formula
[0066]
[0067] 1) Prepare standard solution samples
[0068] A certain amount of urinary microalbumin was dissolved in Tris buffer (pH 8.0, buffer 1 mmol / L) containing 150 mmol / L NaCl to prepare a urine albumin mother solution with a concentration of 1 g / L for later use.
[0069] Take 200 μL of cyanine dye methanol solution with a concentration of 200 μmol / L, and add 9.8 ml of Tris buffer (pH 8.0, buffer 1 mmol / L) containing 20 mmol / L NaCl. Divide the above sample into 10 parts on average, each sample solution is 1mL.
[0070] Take 7 samples among them, add a certain amount o...
Embodiment 2
[0080] In this example, the urine microalbumin concentration of three urine samples is verified. The actual urine microalbumin concentration of each urine sample is as follows: urine sample 1 is 9.18mg / L, urine sample 2 is 32.43mg / L, urine sample 2 is 32.43mg / L, urine Sample 3 was 149.57 mg / L. The cyanine dye used in this example is a compound of the following formula
[0081]
[0082] 1) Prepare standard solution samples
[0083]A certain amount of urinary microalbumin was dissolved in sodium phosphate buffer solution (pH 5.0, buffer concentration 45 mmol) containing 150 mmol / L NaCl to prepare a urine albumin mother solution with a concentration of 1 g / L for later use.
[0084] Take 200 μL of cyanine dye methanol solution with a concentration of 200 μmol / L, and add 9.6 ml of sodium phosphate buffer (pH5.0, 45 mmol) containing 10 mmol / L NaCl. Divide the above sample into 10 parts on average, each sample solution is 0.98mL.
[0085] Take 7 samples, add a certain amount of...
Embodiment 3
[0095] In this example, the urine microalbumin concentration of three urine samples is verified. The actual urine microalbumin concentration of each urine sample is as follows: urine sample 1 is 9.82 mg / L, urine sample 2 is 20.35 mg / L, urine sample 2 is 20.35 mg / L, urine Sample 3 was 78.26mg / L. The cyanine dye used in this example is a compound of the following formula
[0096]
[0097] 1) Prepare standard solution samples
[0098] Dissolve a certain amount of urinary microalbumin in triethanolamine buffer (pH 7.0, buffer concentration 30mmol / L) containing 150mmol / L NaCl and 30mmol / L KCl to prepare a urine albumin stock solution with a concentration of 1g / L ,spare.
[0099] Take 2 mL of cyanine dye methanol solution with a concentration of 200 μmol / L, and add 6 mL of triethanolamine buffer solution (pH 7.0, buffer concentration 30 mmol). Divide the above sample into 10 parts on average, each sample solution is 0.8mL.
[0100] Take 7 of the samples, add a certain amount ...
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