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Kit for determining microalbuminuria and preparation method thereof

A urine microalbumin and kit technology, applied in the fields of medicine and biochemistry, can solve the problems of complicated operation and low measurement accuracy, and achieve the effects of improving sensitivity, facilitating dispersion and stability

Inactive Publication Date: 2016-10-26
ANHUI IPROCOM BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The technical problem to be solved by the present invention is to provide a test kit for measuring urinary microalbumin and its preparation method in order to overcome the defects of complicated operation and low measurement accuracy in the prior art

Method used

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  • Kit for determining microalbuminuria and preparation method thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0070] The kit of the present invention includes two independent liquid components of reagent R1 and reagent R2, wherein

[0071] Reagent R1:

[0072] PBS buffer 90mmol / L

[0073] Polyethylene glycol-8000 50g / L

[0074] Sodium chloride 100 mmol / L

[0075] Sodium azide 0.4 g / L

[0076] Its solvent is purified water

[0077] Reagent R2:

[0078] PBS buffer 90 mmol / L

[0079] Glycerin 20 ml / L

[0080] Tween-80 12 mL / L

[0081] Sodium chloride 200 mmol / L

[0082] Sodium azide 0.4 g / L

[0083] Anti-albumin antibody 11 ml / L

[0084] Its solvent is purified water.

Embodiment 2

[0086] The kit of the present invention includes two independent liquid components of reagent R1 and reagent R2, wherein

[0087] Reagent R1:

[0088] Tris buffer 150 mmol / L

[0089] Polyethylene glycol-2000 35 g / L

[0090] Sodium chloride 30 mmol / L

[0091] Phenol 0.7 g / L

[0092] Its solvent is purified water

[0093] Reagent R2:

[0094] Tris buffer 32 mmol / L

[0095] Gum Arabic 19 ml / L

[0096] Polyoxyethylene phenyl ether 10mL / L

[0097] Potassium chloride 178 mmol / L

[0098] Sodium Benzoate 0.2 g / L

[0099] Anti-albumin antibody 3.0 ml / L

[0100] Its solvent is purified water.

Embodiment 3

[0102] Preparation and use of the kit

[0103] 1. Prepare the reagent according to the following components:

[0104] Reagent R1:

[0105] PBS buffer 90mmol / L

[0106] Polyethylene glycol-8000 50g / L

[0107] Sodium chloride 100 mmol / L

[0108] Sodium azide 0.4 g / L

[0109] Its solvent is purified water

[0110] Reagent R2:

[0111] PBS buffer 90 mmol / L

[0112] Glycerin 20 ml / L

[0113] Tween-80 12 mL / L

[0114] Sodium chloride 200 mmol / L

[0115] Sodium azide 0.4 g / L

[0116] Anti-albumin antibody 11 ml / L

[0117] Its solvent is purified water;

[0118] 2. Automatic biochemical analyzer parameter setting

[0119] (a) Detection temperature: 37℃;

[0120] (b) Detection wavelength: main wavelength 340nm;

[0121] (c) Reaction time: 10min, among which, the incubation time is 5min, the absorbance A1 is measured and read immediately after adding the reagent R2, the absorbance A2 is read after 5min, and the absorbance change ΔA = A2-A1 is calculated;

[0122] (d) Rea...

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Abstract

The invention discloses a kit for determining microalbuminuria and a preparation method thereof. The kit comprises dual-liquid components of a reagent R1 and a reagent R2 which are independent from each other. The reagent R1 is prepared from a buffer solution, an accelerator, inorganic salt ions and a corrosion remover; the reagent R2 is prepared from a buffer solution, a suspending agent, a surface active agent, inorganic salt ions, a corrosion remover and anti-albumin antibodies, and a solvent of the reagent R2 is purified water. The preparation method comprises the steps that the reagents are prepared according to the component content; a to-be-tested sample is mixed with the reagent R1 and the reagent R2 to be subjected to a sufficient reaction; a fully automatic biochemical analyzer is used for determining an absorptivity difference obtained after the reaction; according to an absorptivity change value, the concentration of microalbuminuria in the sample is worked out. The kit has the advantages of being convenient to operate, high in accuracy and the like.

Description

technical field [0001] The invention relates to the technical fields of medicine and biochemistry, in particular to a kit for measuring urinary microalbumin and a preparation method thereof. Background technique [0002] Microalbuminuria refers to the presence of microalbumin in the urine. Albumin is a normal protein in the blood, but only a very small amount of albumin appears in the urine under physiological conditions. Microalbuminuria reflects abnormal leakage of protein from the kidneys. . [0003] Urinary microalbumin is a symptom of early kidney damage in diabetic nephropathy, hypertensive nephropathy and other diseases. No matter what kind of disease is caused by urinary microalbumin, it is the damage of intrinsic kidney cells caused by different starting reasons, which makes the kidney intrinsically damaged. The structure of cells changes, and the function changes with the change of structure, which is reflected in the urine. [0004] Under normal conditions of hu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/31G01N33/68
CPCG01N21/31G01N33/6827
Inventor 蔡晓辉庄庆华吴铮徐运
Owner ANHUI IPROCOM BIOTECH CO LTD
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