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Application of bupleurum polysaccharide in preparation of drugs used for preventing and treating diabetic nephropathy

A technology for diabetes medicine and diabetic nephropathy, which is applied in the field of traditional Chinese medicine and can solve problems such as the efficacy of Bupleurum polysaccharides that have not been reported

Active Publication Date: 2016-05-11
FUDAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Taking a comprehensive view of the research at home and abroad, there is no report on the efficacy of Bupleurum polysaccharides in preventing and treating diabetic nephropathy.

Method used

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  • Application of bupleurum polysaccharide in preparation of drugs used for preventing and treating diabetic nephropathy
  • Application of bupleurum polysaccharide in preparation of drugs used for preventing and treating diabetic nephropathy
  • Application of bupleurum polysaccharide in preparation of drugs used for preventing and treating diabetic nephropathy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Take 100g of Bupleurum herb, extract by cold soaking with 95% ethanol, put the dregs in a ventilated place at room temperature to dry, then extract with hot water for 3 times, filter, combine the extracts, concentrate, centrifuge, and the supernatant is washed with trichloro Remove free protein with acetic acid, centrifuge, dialyze the supernatant with water for 3 days, concentrate the dialysate to a small volume, add ethanol to 80% alcohol content, centrifuge, precipitate and freeze-dry to obtain the total polysaccharide, the product yield is more than 3%, the polysaccharide content More than 65%.

[0052]The Bupleurum medicinal material can be selected from Bupleurum chinense DC or Bupleurumsmithiivar.parvifolium.

Embodiment 2

[0054] Fifty-six C57BL / 6 mice (8 weeks old) were randomly divided into 6 groups (groups A, B, C, D, E, and F) according to body weight: group A was the normal control group, and group B was given Bupleurum polysaccharide alone. Medicine group (normal mice were given Bupleurum polysaccharide, and the dosage was 60mg·kg -1 ), group C is the model group, group D is the model group + Bupleurum polysaccharide 30mg·kg -1 Dosage group, group E is the model group + Bupleurum polysaccharide 60mg·kg -1 Dosage group, F group is the model group + glibenclamide positive group (7.2mg·kg -1 ), each group ranging from 8-12. Taking the day before modeling as Day-1, after the mice were fasted overnight for 15 hours, the models were established in groups C, D, E, and F, and the dose of STZ (Streptozotocin, streptozotocin) 100mg / kg was used for two consecutive days Intraperitoneal injection (0.1mL·10g -1 ). The mice in groups A and B were injected with the same amount of normal saline to sim...

Embodiment 3

[0056] The renal tubular cells were seeded, adhered to the wall overnight, and starved for 24 hours. The experiment was divided into 10 groups. They were: Normal group (normal control group); TGF-β group (model group); SB group (single plus TGF-β inhibitor); BPs20 group (single plus Bupleurum 20 μg / mL group); BPs40 group (single plus Bupleurum 40μg / mL group); BPs80 group (bupleurum 80μg / mL alone group); SB-TGF group; BPs20-TGF group; BPs40-TGF group; BPs80-TGF group. After 48 hours of intervention in each group, the cells were fixed with 4% paraformaldehyde, and the cell morphology was observed and photographed under an inverted microscope.

[0057] The results of animal experiments show that Bupleurum polysaccharides can improve the body weight of diabetic model animals, significantly reduce blood sugar and urine sugar levels, significantly improve insulin secretion, lower serum creatinine levels, reduce urine microalbumin secretion, and lower kidney index. The results of pa...

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Abstract

The invention specifically relates to application of bupleurum polysaccharide in preparation of drugs used for preventing and treating diabetic nephropathy, which belongs to the field of traditional Chinese medicine. According to results of integral experiments and in-vitro experiments, bupleurum polysaccharide extract has obvious prevention and treatment effect on diabetic nephropathy. Bupleurum polysaccharide can improve the body weight of diseased animals, substantially reduce the level of blood sugar and urine sugar, obviously promote secretion of insulin, decrease the level of serum creatinine, reduce secretion level of microalbuminuria and the renal index and enhance renal pathological symptoms, thereby exerting blood sugar-reducing and kidney-protecting effect. Bupleurum polysaccharide can reverse tubular cell transdifferentiation causec by TGF-beta. The bupleurum polysaccharide can be used for preparation of drugs used for preventing and treating diabetes and renal complications caused by diabetes.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine, and relates to a new medicinal application of the traditional Chinese medicine Bupleurum polysaccharide. It specifically relates to the use of Bupleurum polysaccharide in the preparation of medicines for preventing and treating diabetic nephropathy Background technique [0002] Diabetes mellitus is a series of systemic metabolic disorder syndromes characterized by hyperglycemia and caused by absolute or relative insufficiency of insulin secretion. The statistical results of the Chinese Center for Disease Control and Prevention in 2010 show that the prevalence of diabetes among residents aged 18 and over in my country is 9.65%, and the estimated number is about 96.9 million. my country has become the country with the largest population of diabetes in the world (Kang Jihong, TiaoGuan et al. Translational Medicine Research, 2012,2(3):1-24.). With the advancement of urbanization and popul...

Claims

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Application Information

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IPC IPC(8): A61K31/715A61P3/10A61P13/12
Inventor 翁鸿博力弘潘玲玉陈道峰刘珍珍章蕴毅
Owner FUDAN UNIV
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