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Urine microalbumin/urine creatinine detection kit

A urine microalbumin and detection kit technology, which is applied in the direction of chemiluminescence/bioluminescence, and analysis by making materials undergo chemical reactions, can solve the problems of inaccurate measurement results and cumbersome ACR measurement, and achieve accurate and linear measurement Wide, easy-to-automate effects

Active Publication Date: 2018-05-29
GUANGZHOU JINDE BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The purpose of the present invention is to overcome the deficiencies of the prior art and provide a urine microalbumin / urine creatinine detection kit to solve the problems of cumbersome ACR measurement and inaccurate measurement results

Method used

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  • Urine microalbumin/urine creatinine detection kit
  • Urine microalbumin/urine creatinine detection kit
  • Urine microalbumin/urine creatinine detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] As an embodiment of the urine microalbumin / urine creatinine detection kit of the present invention, the urine microalbumin / urine creatinine detection kit of the present embodiment is:

[0054] Immobilized rabbit anti-human serum albumin polyclonal antibody: rabbit anti-human serum albumin polyclonal antibody is coated on the luminescent plate;

[0055] Reagent R1 includes components at the following concentrations: HEPES buffer 0.02mol / L (pH is 7.0), creatinase 20KU / L, sarcosine oxidase 10KU / L, trehalose 2wt%, Tween 20 0.003wt% , catalase 1.0KU / L, bilirubin oxidase 2.0KU / L, vitamin C oxidase 10KU / mL, DMSO 0.5wt% and Proclin300 0.045wt%;

[0056] Reagent R2 includes components at the following concentrations: TAPS buffer 0.05mol / L (pH 8.0), creatinine 30KU / L, NaN 3 0.006wt%, Tween 80 0.008wt%, Trehalose 1.0wt% and Proclin3000.045wt%;

[0057] Luminescent substrate 1:1,2-dioxetaneboronic acid;

[0058] Reagent R3 includes components in the following concentrations: PB...

Embodiment 2

[0062] As an embodiment of the urine creatinine / urine microalbumin detection kit of the present invention, the urine microalbumin / urine creatinine detection kit of the present embodiment is:

[0063] Immobilized rabbit anti-human serum albumin polyclonal antibody: rabbit anti-human serum albumin polyclonal antibody is coated on the luminescent plate;

[0064] Reagent R1 includes components at the following concentrations: Tris-HCl buffer 0.01mol / L (pH is 6.0), creatinase 10KU / L, sarcosine oxidase 5KU / L, trehalose 2wt%, dodecyl - β-D maltoside 0.005wt%, catalase 0.1KU / L, bilirubin oxidase 3KU / L, vitamin C oxidase 5KU / mL, DMSO 0.5wt% and Proclin3000.2wt%;

[0065] Reagent R2 includes components at the following concentrations: TAPS buffer 0.05mol / L (pH 8.0), creatinine 10KU / L, dodecyl-β-D maltoside 0.005wt%, trehalose 2wt% and Proclin300 0.2 wt%;

[0066] Luminescent substrate 1:1,2-dioxetaneboronic acid;

[0067] Reagent R3 includes components in the following concentrations...

Embodiment 3

[0071] As an embodiment of the urine microalbumin / urine creatinine detection kit of the present invention, the urine microalbumin / urine creatinine detection kit of the present embodiment is:

[0072] Immobilized rabbit anti-human serum albumin polyclonal antibody: rabbit anti-human serum albumin polyclonal antibody is coated on the luminescent plate;

[0073] Reagent R1 includes components at the following concentrations: PBS buffer 0.1 mol / L (pH 7.5), creatinase 20 KU / L, sarcosine oxidase 8 KU / L, trehalose 0.5 wt%, dodecyl- β-D maltoside 0.005wt%, bilirubin oxidase 1KU / L, vitamin C oxidase 5KU / mL, DMSO 2wt% and Proclin300 0.045wt%;

[0074] Reagent R2 includes components at the following concentrations: PBS buffer 0.1mol / L (pH is 7.5), creatinine 30KU / L, dodecyl-β-D maltoside 0.005wt%, trehalose 0.1wt% and Proclin300 0.045wt%;

[0075] Luminescent substrate 1:1,2-dioxetaneboronic acid;

[0076] Reagent R3 includes components at the following concentrations: phosphate buffe...

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Abstract

The invention discloses a urine microalbumin / urine creatinine detection kit. The kit comprises a urine creatinine detection reagent and a urine microalbumin detection reagent, wherein the urine creatinine detection reagent comprises a reagent R1 used for eliminating endogenous creatine and sarcosine, a reagent R2 used for converting creatinine into creatine and a luminescent substrate 1 used for detecting hydrogen peroxide; the urine microalbumin detection reagent comprises an immobilized rabbit-anti-human serum albumin polyclonal antibody, a reagent R3 used for binding urine microalbumin andthe immobilized rabbit-anti-human serum albumin polyclonal antibody, an alkaline phosphatase labelled reagent R4 used for binding with urine microalbumin and a luminescent substrate 2 used for detecting alkaline phosphatase. Urine microalbumin and urine creatinine are detected with a glow-type chemiluminiscence method; the detection method can detect two indexes on a chemiluminescence instrument and enables measurement to be more accurate, more convenient and rapider.

Description

technical field [0001] The invention relates to the technical field of in vitro diagnostic reagents, in particular to a urine microalbumin / urine creatinine detection kit. Background technique [0002] ACR (urine microalbumin / urine creatinine ratio) plays an important role in judging the early diagnosis of diabetic nephropathy. There are two main methods for detecting this indicator: one is through the urine microalbumin / urine creatinine urinalysis test card To measure the ACR value, the second is to measure the values ​​of urine microalbumin and urine creatinine, and then obtain their values ​​by calculation. Urine microalbumin measurement methods include enzyme-linked immunosorbent assay, radioimmunoassay, immunoturbidimetry, colloidal gold method, transmission turbidimetry and immunoturbidimetry; creatinine detection methods mainly include dry chemical and enzymatic methods. [0003] The shortcomings of the existing technologies and measurement methods for the detection o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/76
CPCG01N21/76
Inventor 齐宗献李民友丘传添林伟荣罗宁
Owner GUANGZHOU JINDE BIOTECH
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