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Kit for measuring microalbuminuria by adopting immune competition turbidimetry

A urine microalbumin and kit technology, which is applied in the field of kits for the detection of urinary microalbumin by immunocompetitive turbidimetry, can solve problems such as unsatisfactory application, avoid false negative results, avoid hook effects, and produce The effect of simple process

Inactive Publication Date: 2018-02-27
HUNAN HY BIOPOCT TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, ordinary turbidimetric method or latex-enhanced turbidimetric method is used to measure the content of trace albumin in urine. 2mg / L-300mg / L, the above two methods, when the concentration of human albumin in the sample exceeds 3000mg / L, the value measured by the kit will often be lower than 100mg / L, but because the concentration range of clinical samples is relatively large wide, its concentration is 2mg / L-10g / L, and because the general immunoturbidimetric method has a hook effect, it is not very ideal for clinical application

Method used

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  • Kit for measuring microalbuminuria by adopting immune competition turbidimetry
  • Kit for measuring microalbuminuria by adopting immune competition turbidimetry

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Experimental program
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Embodiment 1

[0037] Reagent 1: human albumin-labeled latex microspheres, latex microsphere particle size 80nm, latex microsphere concentration 0.1g / L, human albumin concentration 400mg / L; Reagent 2: rabbit anti-human albumin polyclonal antibody concentration 600mg / L L, boric acid concentration 25mmol / L (pH7.5), sodium chloride concentration 0.2mol / L, Tween-20 concentration 2ml / L, sodium azide concentration 0.1g / L. The ratio of sample to reagent is sample (human urine): reagent 1: reagent 2: 20:240:80. The specific measurement parameters of the automatic biochemical analyzer are: measuring instrument AU640, sample reagent ratio: 20:240:80, calibrator fitting method spline, wavelength 600nm, reading point 12-27.

[0038] The calibration results are shown in Table 1 below, and the calibration curve can be drawn accordingly.

[0039] Table 1 Calibrator Calibration Result Values

[0040] Concentration of calibrator ㎎ / L

[0041] 1) Sample dilution verification:

[0042] Mix the low-va...

Embodiment 2

[0057] Reagent 1: human albumin-labeled latex microspheres, latex microsphere particle size 152nm, latex concentration 0.05g / L, human albumin concentration 80mg / L; reagent 2: rabbit anti-human albumin polyantibody, antibody affinity purification, The antibody concentration is 200mg / L, the sodium chloride concentration is 0.6mol / L, the Tween-20 concentration is 10ml / L, and the ProClin 300 concentration is 0.1g / L. The ratio of sample to reagent is sample (human urine): reagent 1: reagent 2: 20:240:80. The specific measurement parameters of the automatic biochemical analyzer are: measuring instrument AU640, sample reagent ratio: 20:240:80, calibrator fitting method spline, wavelength 600nm, reading point 12-27.

[0058] The calibration results are shown in Table 1, and the calibration curve can be drawn accordingly.

[0059] Table 1 Calibrator Calibration Result Values

[0060] Concentration of calibrator ㎎ / L

[0061] 1) Sample dilution verification:

[0062] Mix the...

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Abstract

The invention provides a kit for measuring microalbuminuria by adopting immune competition turbidimetry. The kit comprises an anti-human albumin antibody, human albumin marked leatexbeads and a buffersolution. The anti-human albumin antibody and the human albumin marked leatexbeads are separately placed. The particle size of the leatexbeads is 80-267 nm. When the kit is used for measuring microalbuminuria in human urine, the maximum linear range is 5 mg / L-1500 mg / L. The kit can meet screening of clinical normal people, and can also avoid result underestimation or even false negative result arising from a hook effect caused by antigen excess.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a kit for detecting urinary microalbumin by immunocompetitive turbidimetry. Background technique [0002] Immunoturbidimetry is a dynamic assay method for antigen-antibody binding. Its basic principle is: when the antigen and antibody react in a special dilution system and the ratio is appropriate (generally stipulates that the antibody is excessive), the soluble immune complex formed in the dilution system is promoted. Under the action of the polymerization agent (polyethylene glycol, etc.), it precipitates from the liquid phase to form particles, which makes the reaction liquid turbid. When the antibody concentration is fixed, the amount of the formed immune complex increases with the increase of the amount of antigen in the test sample, and the turbidity of the reaction solution also increases accordingly. By measuring the turbidity of the reaction solution and comparing it with ...

Claims

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Application Information

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IPC IPC(8): G01N33/543G01N33/532
CPCG01N33/54346G01N33/532
Inventor 史丙华姜志华陈玲强王延英
Owner HUNAN HY BIOPOCT TECH CO LTD
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