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Anti-Human Albumin Mixed Antibody and Assay Kit

A kit and serum albumin technology, applied in the direction of anti-animal/human immunoglobulin, biological testing, measuring devices, etc., can solve the problems of high linear upper limit, low detection limit, etc., and achieve high detection range, high sensitivity, The effect of avoiding missed diagnosis

Active Publication Date: 2022-04-12
SHENZHEN NEW INDS BIOMEDICAL ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The main purpose of the present invention is to provide an anti-human albumin (anti-human ALB) mixed antibody and assay kit to solve the problem that the immunoturbidimetric method in the prior art cannot have a low detection limit and a high linear upper limit at the same time (1875~2500mg / L) problem

Method used

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  • Anti-Human Albumin Mixed Antibody and Assay Kit
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  • Anti-Human Albumin Mixed Antibody and Assay Kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] A test kit for measuring mALB, comprising reagent R1 and reagent R2,

[0063] The composition of reagent R2 is: 100mmol / L Tris-HCl buffer solution, pH 7.8;

[0064] The concentration is 900mg / L mixed antibody, anti-human ALB1-2:anti-human ALB2-2 antibody mass ratio=7:3;

[0065] BSA, 2g / L;

[0066] Trehalose, 6g / L;

[0067] ProClin 300, 0.5g / L;

[0068] EDTA-Na 2 , 30mmol / L;

[0069] Sodium chloride, 10g / L.

[0070] The composition of reagent R1 is: 100mmol / L Tris-HCl buffer solution with pH 7.8, 40g / L polyethylene glycol (molecular weight: 6000), 50mmol / L sodium chloride, 2% TritonX-100, 0.02% sodium azide.

Embodiment 2

[0072] A test kit for measuring mALB, comprising reagent R1 and reagent R2,

[0073] The composition of reagent R2 is: 20mM Na 2 HPO 4 -NaH 2 PO 4 Buffer, pH 7.0;

[0074] The concentration is 800mg / L mixed antibody, anti-human ALB1-2:anti-human ALB2-2 antibody mass ratio=7:3;

[0075] EDTA-Na 4 , 80mmol / L;

[0076] Trehalose, 8g / L;

[0077] BSA, 2g / L;

[0078] Sodium chloride, 10g / L;

[0079] ProClin 300, 0.5g / L.

[0080] The composition of reagent R1 is: 20mM Na at pH 7.0 2 HPO 4 -NaH 2 PO 4 Buffer, 20g / L polyethylene glycol (molecular weight: 8000), 50mmol / L sodium chloride, 0.1% TritonX-20, 0.02% sodium azide.

Embodiment 3

[0082] A test kit for measuring mALB, comprising reagent R1 and reagent R2,

[0083] The composition of reagent R2 is: 100mmol / L Tris-HCl buffer solution, pH 7.8;

[0084] The concentration is 1000mg / L mixed antibody, anti-human ALB1-1:anti-human ALB2-1 antibody mass ratio=7:3;

[0085] Sodium potassium tartrate, 30mmol / L;

[0086] Sucrose, 5g / L;

[0087] BSA, 2g / L;

[0088] Sodium chloride, 10g / L;

[0089] ProClin 300, 0.5g / L.

[0090] The composition of reagent R1 is: 100mmol / L Tris-HCl buffer solution with pH 7.8, 40g / L polyethylene glycol (molecular weight: 6000), 50mmol / L sodium chloride, 2% TritonX-100, 0.02% sodium azide.

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Abstract

The invention provides an anti-human albumin mixed antibody and a test kit. The mixed antibody includes anti-human albumin antibody 1 (anti-human ALB antibody 1) and anti-human albumin antibody 2 (anti-human ALB antibody 2). The lower linear limit of anti-human ALB antibody 1 for mALB detection is 5-30 mg / L, The linear upper limit of the detection of mALB by anti-human ALB antibody 2 is 1800-2000 mg / L. The mixed antibody of the present application can take into account the advantages of high sensitivity and high detection range, and at the same time achieve an unexpected synergistic effect beyond the characteristics of the two antibodies, so that it can meet the screening of normal people in clinical applications (the lowest value can be measured to 5mg / L) , reduce the dilution work of higher concentration samples (1875-2500mg / L), and also avoid missed diagnosis of patients with high concentrations (the HOOK effect does not appear at high concentrations).

Description

technical field [0001] The invention relates to the field of in vitro diagnosis, in particular to an anti-human albumin mixed antibody and a measurement kit. Background technique [0002] The excretion of total protein in urine is normal, the urine routine protein quantification is negative, but the excretion of urinary albumin is increased, which is called microalbuminuria. The concentration of urinary microalbumin in normal people can be lower than 20mg / L, while the concentration of clinical high-concentration urine microalbumin samples can reach 4000-5000mg / L. Detection requirements for high-concentration samples. [0003] Currently commonly used methods for the determination of urinary microalbumin include colloidal gold method, common immunoturbidimetric method and latex immunoturbidimetric method. Among them, the colloidal gold method can only give qualitative or semi-quantitative results; although the ordinary immunoturbidimetric method can be quantitative, but the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K16/18G01N33/68
CPCC07K16/18G01N33/68G01N2333/765
Inventor 时敏廖洁韦锐罗娅钟小年
Owner SHENZHEN NEW INDS BIOMEDICAL ENG
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