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Beta 2-microglobulin detection kit

A technology for detecting kits and microglobulins, applied in the field of medical immunity, can solve the problems of reduced quality of reagents, prone to coalescence, poor stability of reagents, etc., and achieves the effects of simple detection, high sensitivity and low production cost

Inactive Publication Date: 2016-05-25
宁波天康生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, the connection between antibody and latex is usually carried out by chemical coupling method. As an R2 reagent, it is very easy to coalesce, which makes the quality of the reagent decrease with the prolongation of storage time.
Poor reagent stability

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Example 1: Preparation of β2-microglobulin detection kit

[0024] Kit of the present invention relates to the main material of reagent as follows:

[0025] 1. β2-microglobulin antibody: polyclonal antibody (commercially available).

[0026] 2. Latex: Experiments were carried out using polystyrene latex particles (commercially available) with a diameter of 80-200 nm and carboxyl groups.

[0027] The preparation of the main reagent of this embodiment is as follows:

[0028] Reagent R1: phosphate buffer solution containing 2.5% PEG6000 (polyethylene glycol 6000), 95mmol / L NaCl, this reagent is a colorless transparent solution.

[0029] Reagent R2: polystyrene latex particles with a particle diameter of 120 nm were sensitized with an anti-human β2-microglobulin antibody. The reagent is a milky white solution. Specific steps are as follows:

[0030] 1. Take 1ml (100mg / ml) latex, wash 3 times with 0.02M (mol / L), pH 5.0 MES solution (2-morpholineethanesulfonic acid ...

Embodiment 2

[0037] Example 2: Determination of β2-microglobulin

[0038] Detection tool: Hitachi 7060 automatic analyzer.

[0039] Analysis method: two-point endpoint method; main wavelength: 570nm, secondary wavelength: 700nm; sample volume: 2ul (microliter); R1: 200ul; R2: 50ul; reaction direction: rising; measurement temperature: 37°C; sample mixed with R1 After mixing, read the absorbance A1 at 10 seconds; add R2 at 3-5 minutes, and read the absorbance A2 after 5 minutes. The reaction absorbance was calculated as the difference between A2 and A1.

[0040] Calculation method: multi-point calibration, the dose / response curve is made according to the absorbance and reference serum value, and the sample content can be calculated on the dose / response curve according to its absorbance value.

Embodiment 3

[0041] Example 3: Performance evaluation of β2-microglobulin detection kit

[0042] 1. Analytical Sensitivity Assessment

[0043] Use 5% bovine serum albumin solution as a blank sample, and the blank sample should not contain the analytes. 20 consecutive detections were performed on the biochemical analyzer, and the mean and standard deviation SD of the 20 results were calculated. The detection limit of the reporting method is the mean value of the blank plus two standard deviations (+2SD). As can be seen from Table 1, the sensitivity is 0.053mg / L.

[0044] Table 1

[0045]

[0046] 2. High value linear evaluation

[0047] Mix 1 part of low-value serum (0.5mg / L) and 1 part of high-value serum (25mg / L) at 9:1, 4:1, 2:1, 1:1, 1:2, 1:3, 1:1 4, 1: 9 (sample sequence number is No. 1~6), prepare 6 samples with different concentrations, each sample is measured twice, as seen from Table 2, the highest detection range of the detection kit of the present invention can reach 20.1...

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Abstract

The invention discloses a beta 2-microglobulin detection kit which comprises a reagent R1 and a reagent R2, and the reagent R1 is a buffer solution; the reagent R2 is a mixture of beta 2-microglobulin antibody sensitization polystyrene latex particles and a buffer solution. The detection kit has the advantages that the detection is simple and rapid, the sensitivity is high, the accuracy is good, the disturbance resistance is high and the production cost is low. An adopted beta 2-microglobulin detection method is latex enhanced immunoturbidimetry, the beta 2-microglobulin detection is more economical, convenient and rapid with the method, and the beta 2-microglobulin detection kit is suitable for automatic biochemistry analyzers in vast majority of hospitals, and particularly suitable for realizing rapid quantitative detection on emergency treatment.

Description

technical field [0001] The invention belongs to the field of medical immunity, and relates to an immunological detection reagent, and furthermore, the invention relates to a β2-microglobulin detection kit. Background technique [0002] β2-microglobulin is mainly used for renal function evaluation and as a non-specific tumor marker. Increased urine concentration and normal serum level are due to decreased renal tubular reabsorption, which is seen in renal tubular diseases such as Fanconi syndrome, Lowe syndrome, Chronic chromium poisoning, acute tubular necrosis, cystine ornithosis, kidney transplantation, acute or chronic pyelonephritis, and cystitis is normal; serum levels are increased and urine concentrations are normal due to glomerulopathy and decreased filtration rate, seen in acute or chronic Nephritis; both serum level and urine level concentration are increased, which is seen in increased production and exceeding the reabsorption capacity of renal tubules, seen in m...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/577G01N33/531
CPCG01N33/6854G01N33/531G01N33/577
Inventor 陆雪龙宋高峰李清华周裕国
Owner 宁波天康生物科技有限公司
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