Immunochromatographic test strip for rapidly detecting malaria and preparation method thereof

A technology for immunochromatography and test strips, applied in the field of medical testing, can solve the problems of inability to display test results in multiple styles, difficult to distinguish test lines and quality control lines, and complicated preparation process of colloidal gold labels, so as to improve sensitivity and test results. accuracy, improved preventive capabilities, and significant economic and social benefits
CN102087294AActive Publication Date: 2011-06-08GUANGZHOU WONDFO BIOTECH
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Patent Information

Authority / Receiving Office
CN Ā· China
Current Assignee / Owner
GUANGZHOU WONDFO BIOTECH
Publication Date
2011-06-08

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Abstract

The invention discloses an immunochromatographic test strip for rapidly detecting malaria and a preparation method thereof. The test strip is formed by sticking a sample pad, a labeling pad, a coating membrane and absorbent paper to a substrate in sequence through lap joint, wherein colored latex particle labeled plasmodium falciparum histidine-rich protein II monoclonal antibody and non-plasmodium falciparum lactic dehydrogenase monoclonal antibody are coated on the labeling pad; the coating membrane comprises detection regions and a control region; the detection regions are coated by plasmodium falciparum histidine-rich protein II monoclonal antibody and non-plasmodium falciparum lactic dehydrogenase monoclonal antibody with epitopes different from the epitopes of the monoclonal antibodies on the labeling pad; and the control region is coated by an anti-mouse IgG (immunoglobulin G) monoclonal antibody. The test strip improves the accuracy and convenience of malaria screening, is simple and convenient to operate and has the advantages of rapidness, simpleness, convenience and intuition.
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Description

technical field

[0001] The invention belongs to the field of medical testing. Specifically, the invention relates to an immunochromatographic detection test strip for rapid joint detection of falciparum malaria (P.f) and non-falciparum malaria (P.v, P.o, P.m) and a preparation method thereof. Background technique

[0002] Malaria is a vector-borne infectious disease that seriously endangers human health. There are four kinds of Plasmodium parasites in the human body: Plasmodium falciparum (PlasmodiĪ¼m falciparĪ¼m), Plasmodium vivax (PlasmodiĪ¼m vivax), Plasmodium malariae (PlasmodiĪ¼m malariae) and Plasmodium ovale. The former two are the most common in our country. Blood testing for Plasmodium is still the most reliable method for diagnosing malaria so far. This method can identify the species and stage of the parasite, but it is time-consuming and laborious, and requires skilled technicians and certain laboratory conditions. The laboratory diagnosis of malaria mainly relies ...

Claims

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