PCT/SAA combined test paper strip for rapid detection and preparation method thereof

A test strip and test strip technology are applied in the field of test strips for PCT/SAA combined rapid detection and their preparation, which can solve the problems of not being able to detect PCT/SAA at the same time, and achieve high clinical reference value, wide application range, and diagnosis. Accurate results

Inactive Publication Date: 2015-04-29
MAANSHAN GUOSHENG BIO TECH
View PDF7 Cites 10 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The purpose of the present invention is to solve the problems mentioned in the background technology, to facilitate the combined detection of procalcitonin (procalcitonin, PCT) and Serum amyloid A protein (serum amyloi

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • PCT/SAA combined test paper strip for rapid detection and preparation method thereof
  • PCT/SAA combined test paper strip for rapid detection and preparation method thereof
  • PCT/SAA combined test paper strip for rapid detection and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] A test strip for combined rapid detection of PCT / SAA, the preparation method of which is as follows:

[0027] 1) Sample pad pretreatment: evenly coat the sample pad treatment solution containing Tris, casein sodium salt, and PVP-10 on the glass cellulose membrane, dry it at 37 degrees Celsius, and store it in a sealed bag at room temperature.

[0028] 2) Pre-treatment of gold conjugate pad: evenly coat the gold conjugate pad treatment solution containing Tris, trisodium citrate, Tween 20, etc. Store at room temperature.

[0029] 3) Preparation of gold-conjugated mats: prepare colloidal gold-labeled anti-PCT monoclonal antibody cross-linked products and colloidal gold-labeled anti-SAA monoclonal antibody cross-linked products respectively, mix the two colloidal gold-labeled antibodies, and coat Overlay the gold conjugate pad treated in step 2).

[0030] 4) Preparation of detection line and quality control line on nitrocellulose membrane: use paired anti-SAA monoclonal an...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a test paper strip for (PCT/SAA) combined rapid detection of human procalcitonin/serum amyloid protein A. The test paper strip comprises a detecting card shell, a test strip, a sample pad, a gold conjugate pad coated with two colloidal gold labeled antibodies, a nitrocellulose membrane and water absorbing paper, wherein the nitrocellulose membrane is provided with a T1 detection line coated with a solid-phase matched anti-SAA monoclonal antibody, a T2 detection line coated with a solid-phase matched anti-PCT monoclonal antibody, and a control line C which is parallel to the detection line and coated with a goat anti mouse IgG monoclonal antibody. Two kinds of colloidal gold labeled antibodies are provided, which are respectively a colloidal gold labeled antibody which can be specifically combined with a to-be-detected antigen PCT and an antibody which can be specifically combined with a to-be-detected antigen SAA. The test paper strip disclosed by the invention can be used for simultaneously and rapidly detecting the PCT/SAA in a patient sample in a combined manner and has the advantages of improving the diagnosis accuracy of early inflammatory reaction of infectious diseases and being simple to operate, rapid and convenient.

Description

technical field [0001] The invention belongs to the field of biomedicine, and relates to a test strip for combined rapid detection of human procalcitonin (PCT) and serum amyloid A protein (SAA) and a preparation method thereof, specifically A colloidal gold immunochromatography method using the principle of the double-antibody sandwich method, combined with an immunochromatography reader, simultaneously realizes a method for on-site rapid detection of PCT / SAA two items. Background technique [0002] This product uses double-antibody sandwich method and colloidal gold immunochromatography technology to rapidly detect procalcitonin (PCT) and serum amyloid A (SAA) in human whole blood, serum or plasma samples at the same time, and can be used for inflammation and other infections Auxiliary diagnosis of such diseases. [0003] In recent years, with the development of colloidal gold immunochromatography technology and biochemical preparation technology, it has become a reality ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): G01N33/577G01N33/543
CPCG01N33/577
Inventor 刘辉宇张荔
Owner MAANSHAN GUOSHENG BIO TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap