Multi-residue colloidal-gold rapid detection kit, and detection method and application thereof

A detection kit and multi-residue technology, applied in the field of immunology, can solve the problems of long detection time, large application limitations, and cumbersome detection methods.

Inactive Publication Date: 2013-03-20
北京陆桥技术股份有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem solved by the present invention is to provide a detection kit capable of simultaneously detecting multiple antibiotic residues in food and its detection method and application; the main purpose of the invention is to overcome the traditional detection method which is c

Method used

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  • Multi-residue colloidal-gold rapid detection kit, and detection method and application thereof
  • Multi-residue colloidal-gold rapid detection kit, and detection method and application thereof
  • Multi-residue colloidal-gold rapid detection kit, and detection method and application thereof

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Experimental program
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Effect test

Embodiment 1

[0075] The sensitivity experiment of embodiment 1 kit

[0076] 1. Test samples

[0077] 20 copies were randomly selected by the method of "Announcement No. 1163 of the Ministry of Agriculture-7-2009 High Performance Liquid Chromatography for the Detection of Gentamicin Residues in Animal Foods", "GB / T 22969-2008 Streptomycin and Dihydrogen in Milk Powder and Milk Determination of streptomycin and kanamycin residues by liquid chromatography-tandem mass spectrometry" method and "GB / T 21312-2007 Determination of 14 quinolone drug residues in food of animal origin by liquid chromatography-mass spectrometry / mass spectrometry" Methods Sensitivity detection experiments were carried out on negative samples that did not contain such substances.

[0078] 2. Preparation of Contaminated Samples

[0079] 1. Preparation of standard solution

[0080] Prepare mixed standard solutions of gentamicin and kanamycin respectively, with final concentrations of 10 μg / mL gentamicin and 10 μg / mL kan...

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Abstract

The invention discloses a multi-residue colloidal-gold rapid detection kit and a detection method and application thereof, belonging to the field of immunology. The kit provided by the invention mainly comprises a multi-residue colloidal-gold rapid detection card; the detection card comprises a detector bar and a plastic card shell, and the detector bar is composed of a sample pad, a colloidal-gold antibody binding pad, a nitrocellulose membrane and a water absorbing pad. The colloidal-gold antibody binding pad comprises a colloidal gold labeled gentamycin monoclonal antibody, a colloidal gold labeled kanamycin monoclonal antibody and a colloidal gold labeled fluoroquinolone monoclonal antibody. The central part of the nitrocellulose membrane is coated with a detection line and a control line, wherein the detection line is arranged to be a protein conjugate and the control line is a goat anti-mouse IgG monoclonal antibody. The rapid detection kit provided by the invention can realize simultaneous detection of a plurality of residues only through simple pre-treatment, the whole operation process is simple, the kit is convenient to carry, result determination is rapid and accurate, and the kit is applicable to on-site monitoring and to qualitative screening of considerable samples.

Description

technical field [0001] The invention belongs to the field of immunology, and relates to a kit and a method for rapidly detecting residues of aminoglycoside antibiotic gentamycin in food. Background technique [0002] Since 2008, the safety issue of dairy products has had a huge impact on the whole country and even the whole world. Not only the rights and interests of consumers have been damaged, but also the interests of the entire dairy industry including many dairy farmers have been greatly damaged. The food safety problem in the dairy industry has seriously related to the national economy and people's livelihood of our country and needs to be solved urgently. The safety of dairy products not only needs to start from the source, through various rules and regulations to control the quality layer by layer, but also requires various detection technologies to check layer by layer. Due to the huge amount of raw materials involved in the production of dairy products and their n...

Claims

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Application Information

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IPC IPC(8): G01N33/04G01N33/577
Inventor 唐慧林何艳玲于重楠刘敏王丽丽王丽哲赵瑜
Owner 北京陆桥技术股份有限公司
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