Zika virus NSI antigen and application of Zika virus NSI antigen in preparing fluorescence immunochromatographic reagent

A fluorescent immunochromatography and Zika virus technology, which is applied in the field of fluorescent immunochromatography in medical immunology, can solve the problems of false positive detection results, low detection sensitivity, low antigen specificity, etc., and achieve accurate and reliable detection results , Expand the detection range, and the effect of short time consumption
CN110568178AActive Publication Date: 2019-12-13CHINESE ACAD OF INSPECTION & QUARANTINE
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
CHINESE ACAD OF INSPECTION & QUARANTINE
Publication Date
2019-12-13

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Abstract

The invention relates to a Zika virus NSI antigen and the application of Zika virus NSI antigen in preparing a fluorescence immunochromatographic reagent. The amino acid sequence of the Zika virus NS1antigen is shown as SEQ ID NO. 1. The prepared fluorescence immunochromatographic reagent is a fluorescence immunochromatographic reagent for detecting a Zika virus antibody. A sample pad, a marker pad, a coating pad and an absorption pad are sequentially overlapped and adhered to a bottom lining card, and the marker pad is a glass fiber coated with a fluorescent latex microsphere labeled mouse anti-human IgG monoclonal antibody, and the coating pad is a nitrocellulose membrane coated with a goat anti-rabbit-mouse IgG antibody as a quality control line and a detection line coated with the Zika virus NS1 antigen. The Zika virus NS1 antigen provided by the invention has a better expression effect and is used for preparing the fluorescence immunochromatographic reagent for detecting a Zika virus IgG antibody, and a detection result can be observed by naked eyes after irradiation by ultraviolet rays.
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Description

technical field

[0001] The invention relates to a method for detecting Zika virus NS1 antigen and its application in the preparation of fluorescent immunochromatography reagents, belonging to the technical field of fluorescent immune chromatography in medical immunology. Background technique

[0002] After people are infected with Zika virus (Zika Virus), 1 / 4-1 / 5 of the infected people will develop the disease. The clinical features are mild and mostly flu-like, mainly fever, arthralgia, rash or conjunctivitis, which lasts for several days. However, some patients will develop Guillain-Barré syndrome, which poses a great threat to pregnant women, leading to fetal miscarriage, neonatal microcephaly and even death. Zika virus disease urgently needs prevention and control.

[0003] After Zika virus infection, its symptoms are mild, and about 80% of Zika virus-infected patients will not show any symptoms, which has greatly interfered with clinical diagnosis and the prevention an...

Claims

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