Method for detecting equine coronavirus antibody and special diagnosis kit thereof

Abstract

This invention discloses one method and special agent case to test horse head virus antibody, which comprises the following steps: the horse head virus nuclear shell gene expression product of polyphenylacetylene standard board; reaction board adding the horse blood serum to be tested; adding enzyme label IgG single clone antibody, color development and testing for OD490nm. This invention applies real nuclear expression system to re-create bar virus infected insect cell and to get the virus expression.

Description

Technical field:

[0001] The invention relates to a detection method and kit for animal infectious diseases. Background technique:

[0002] Equine coronavirus (ECV) is a single-stranded positive-sense RNA virus. In 1975, more than 40 cases of infectious disease in newborn foals in the United States, characterized by watery diarrhea, fever and lymphoid tissue lesions, were finally proved to be caused by equine coronavirus (ECV) infection. In 1983, Huang JC et al. isolated ECV from a sick horse with severe diarrhea. Since then, ECV has entered the sight of people's research and made some research progress.

[0003] Establishing a specific and rapid ECV diagnostic method is the premise of controlling and eradicating ECV. At present, Gu Bingquan and others have established an effective RT-PCR diagnostic method in the Australian laboratory, which can detect ECV pathogens in feces and disease materials, with high sensitivity and specificity. On this basis, by optimizing the cond...