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38results about How to "Control happens" patented technology

System and method for disinfecting soilless culture medium

The invention relates to a system and method for disinfecting a soilless culture medium. The system for disinfecting the soilless culture medium comprises a drip irrigation system, a medium groove, a liquid storage pool, a liquid storage barrel and an electrolysis device. The drip irrigation system is arranged above the medium groove. The bottom of the medium groove is connected with the liquid storage pool through a liquid return pipe. Two submerged pumps are arranged at the bottom inside the liquid storage pool. One submerged pump is connected with the liquid storage barrel through a filter. The liquid storage barrel is further connected with an inlet of the electrolysis device through a booster pump. An anode liquid outlet of the electrolysis device is connected with the drip irrigation system through an anode terminal liquid return pipe and a cathode liquid outlet is connected with the liquid storage pool through a cathode terminal liquid return pipe. The invention further provides the method with the system for disinfecting the soilless culture medium adopted. According to the system for disinfecting the soilless culture medium, a disinfecting closed loop and a nutrient solution supply closed loop are obtained through a three-way valve, the automation degree is high, the energy consumption is small, pathogenic microorganism generated in the medium is thoroughly killed, occurrence and transmission of root transmission diseases can be effectively controlled, and residue and pollution are avoided.
Owner:BEIJING RES CENT OF INTELLIGENT EQUIP FOR AGRI

Multiple cropping cultivation method for eggplants

The invention relates to a multiple cropping cultivation method for eggplants. The method comprises the following steps: performing eggplant seedling raising and cultivation; after first eggplants are harvested in a fruit harvesting period, performing pruning, smearing wounds with pasty pesticide ointment to prevent disease infection, and topdressing water full of fertilizer, wherein the pesticide ointment is prepared from 10 to 50 grams of agricultural streptomycin, 10 to 30 grams of 75 percent chlorothalonil and 5 to 40 grams of 20 percent virus A wettable powder, and virus A wettable powder comprises moroxydine hydrochloride and copper acetate; when new side branches are sprouted, performing foliar fertilization, and producing and harvesting second-crop eggplants; then performing pruning, fertilization and the like to harvest all third-crop eggplants according to a cultivation method for the second-crop eggplants. According to the cultivation method, the disease resistance of the eggplants is improved, the planting and harvesting period of the eggplants is greatly prolonged, the yield is 2 to 3 times higher than that of a conventional planting method, the quality of each crop is more stable than that of the conventional planting method, and economic benefits are improved.
Owner:陕西秦灞现代农业开发有限公司

Pesticide composition for preventing and treating crop root nematodosis by foliage spray

The invention relates to a pesticide composition for preventing and treating crop root nematodosis by foliage spray. The pesticide composition comprises effective components including oxamyl and a plant resistance inducer, wherein the plant resistance inducer is a plant growth regulator with a resistance induction effect or any one or a mixture of two of active polysaccharides; the plant growth regulator comprises compound nitrophenolate and diethyl aminoethyl hexanoate; the active polysaccharides comprise chitosan oligosaccharide and glucal; the effective components comprise 0.05-90.0 parts by weight of the oxamyl and 0.05-90.0 parts by weight of the plant resistance inducer. According to the pesticide composition, the synergistic effects of nematode killing and resistance induction are utilized to control the generation and the development of the crop root nematodosis; the pesticide composition is conducted to crop roots by foliage absorption and nematodes are prevented from being invaded, parasitized and reproduced at the roots; meanwhile, damages to crops by toxic secreta of the nematodes are removed and the symptoms of etiolation, withering and the like are relieved; the formation of insect galls at the roots is relieved. The pesticide composition has an obvious preventing and treating effect on systemic translocation insects and leaf diseases of over-ground parts of the crops.
Owner:郭红甫

Fast detection method for high-resistance botrytis cinerea bacterial strains of carbendazim based on LAMP (loop-mediated isothermal amplification) technology

The invention discloses a fast detection method for high-resistance botrytis cinerea bacterial strains of carbendazim. The method can be used for performing dynamic monitoring and early warning on botrytis cinerea bacteria causing gray mold of crops for the resistance groups of the carbendazol. In the method disclosed by the invention, a molecular detection technology having the advantages of fastness, convenience, strong specificity, high sensitivity and low cost is established on the basis of a loop-mediated isothermal amplification technology (LAMP). The detection method comprises the following steps: designing 2 pairs of specific primers on beta tubulin of high-resistance bacterial strains of the botrytis cinerea bacteria for the carbendazol, performing LAMP amplification, and judging whether products are the high-resistance bacterial strains of the botrytis cinerea bacteria for the carbendazol or not according to the colors of reaction products. If the LAMP amplification products appear to be azure, electrophoresis patterns present ladder-shaped strips, and the product amplification is generated, the products are identified as the high-resistance bacterial strains of the botrytis cinerea bacteria for the carbendazol; if the LAMP amplification products present violet, the electrophoresis patterns do not have strips, and the product amplification is not generated, then the products are identified as non high-resistance bacterial strains of the botrytis cinerea bacteria for the carbendazol. The method is simple, convenient, fast, and low in cost and has important actual significance in the resistance risk assessment of the gray mold of the crops and reasonable medication.
Owner:NANJING AGRICULTURAL UNIVERSITY

Multiple RT-PCR method for detecting five sugarcane viruses simultaneously as well as primer and kit thereof

The invention discloses a multiple RT-PCR detection primer group for detecting five kinds of sugarcane viruses simultaneously. The multiple RT-PCR detection primer group for detecting five kinds of sugarcane viruses simultaneously comprises six pairs of PCR primers which aim at five sugarcane viruses such as SCBV, SSV, SCMV, ScYLV and SrMV and one sugarcane reference gene GPADH correspondingly; the six pairs of PCR primers have base sequences as shown in a sequence table SEQ ID NO.1 to SEQ ID NO.12 correspondingly. Therefore, primer concentration and annealing temperature which influence multiple RT-PCR amplification are optimized by a multiple RT-PCR system and a corresponding RT-PCR detection method is established; and by the method, the five viruses for infecting the sugarcane can be detected simultaneously from sugarcane tissues rapidly, accurately, simply and conveniently, and important significance in screening healthy seedling mother plants, monitoring healthy seedling breeding,breeding for disease resistance and predicting field disease epidemic is achieved. In order to facilitate application of the multiple RT-PCR detection primer group, a corresponding kit is designed and assembled, so that popularization and promotion in grass roots are facilitated.
Owner:GUANGXI UNIV

Ultrasonic atomizing device

The invention discloses an ultrasonic atomizing device. The ultrasonic atomizing device comprises an atomizing box, an atomizing chamber, a mist discharging chamber, a mixing chamber, a heating chamber, an ultrasonic transducer, an air pump, a mist discharging spray head, a fan, a heater and an exhaust nozzle, wherein the atomizing chamber, the mist discharging chamber, the mixing chamber and theheating chamber are sequentially arranged in the atomizing box from bottom to top, the ultrasonic transducer is arranged in the atomizing chamber, the air pump is arranged in the mist discharging chamber, the mist discharging spray head is connected with the air pump, the fan and the heater are arranged in the heating chamber, and the exhaust nozzle is used for exhausting hot air in the heating chamber into the mixing chamber. According to the ultrasonic atomizing device, liquid can be atomized into smaller liquid drops through combination of primary ultrasonic and secondary hot air so that the atomized liquid drops can suspend in the air for a long time, and a better use effect is achieved; mist and hot air can be sufficiently mixed by arranging a flow guide mixing assembly, the heating effect of the hot air on the mist can be improved, and droplets with smaller diameters can be formed by the mist; and according to the ultrasonic atomizing device, by arranging an automatic liquid supplementing assembly, solution supplementing can be automatically carried out on the atomizing chamber, so that efficient operation of the ultrasonic atomizing device is maintained.
Owner:SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI

Mixed feed for preventing and curing laying hen contagious viral diseases

The invention relates to the technical field of mixed chicken feed for laying hens, in particular to mixed feed for preventing and curing laying hen contagious viral diseases, prepared from the following materials in parts by weight: 5 to 8 parts of sweet potato leaf, 5 to 8 parts of erigeron breviscapus, 10 to 15 parts of angelica sinensis, 3 to 6 parts of gynostemma pentaphyllum, 5 to 8 parts of medlar, 1 to 2 parts of gastrodia elata, 1 to 2 parts of radix cynanchi bungei, 3 to 6 parts of liquorice, 5 to 8 parts of houttuynia cordata, 10 to 15 parts of isatis root, 5 to 8 parts of folium isatidis, 5 to 8 parts of fructus forsythiae, 1 to 2 parts of aerba lupulin, 3 to 6 parts of semen cuscutae, 1 to 2 parts of selfheal, 3 to 6 parts of morinda officinalis, 3 to 6 parts of eucommia ulmoides, 3 to 6 parts of Chinese yam, 1 to 2 parts of dangshen, 30 to 50 parts of yellow maize, 5 to 8 parts of middlings, 10 to 15 parts of alfalfa powder, 10 to 15 parts of fried soybean, 10 to 15 parts of peanut meal, 1 to 2 parts of calcium lactate, 0.01 to 0.5 parts of probiotics, 0.01 to 0.5 parts of choline chloride, 0.01 to 0.05 parts of Jinxingweita, 0.01 to 0.2 parts of microelement, 0.01 to 1 part of salt and 1 to 5 parts of shell powder. A laying hen using the mixed feed provided by the invention for prevention and curing by traditional Chinese medicine has an illness catching rate of only 0.3 percent; after a sick hen is fed with the mixed feed, the death and culling rate is reduced by over 10 percent, and the veterinary drug residues of eggs laid by the recovered hen do not exceed standards.
Owner:峨眉山市全林农业科技有限公司

A kind of multi-crop cultivation method of eggplant

The invention relates to a multiple cropping cultivation method for eggplants. The method comprises the following steps: performing eggplant seedling raising and cultivation; after first eggplants are harvested in a fruit harvesting period, performing pruning, smearing wounds with pasty pesticide ointment to prevent disease infection, and topdressing water full of fertilizer, wherein the pesticide ointment is prepared from 10 to 50 grams of agricultural streptomycin, 10 to 30 grams of 75 percent chlorothalonil and 5 to 40 grams of 20 percent virus A wettable powder, and virus A wettable powder comprises moroxydine hydrochloride and copper acetate; when new side branches are sprouted, performing foliar fertilization, and producing and harvesting second-crop eggplants; then performing pruning, fertilization and the like to harvest all third-crop eggplants according to a cultivation method for the second-crop eggplants. According to the cultivation method, the disease resistance of the eggplants is improved, the planting and harvesting period of the eggplants is greatly prolonged, the yield is 2 to 3 times higher than that of a conventional planting method, the quality of each crop is more stable than that of the conventional planting method, and economic benefits are improved.
Owner:陕西秦灞现代农业开发有限公司

Multiple detection method and kit for simultaneously detecting three sweet potato viruses

The invention relates to the technical field of sweet potato virus detection, in particular to a multiple detection method and a kit for simultaneously detecting three sweet potato viruses. The kit comprises a 10 * reaction buffer solution, Taq enzyme, upstream primers of the three sweet potato viruses, downstream primers of the three sweet potato viruses and a positive standard substance. The three sweet potato viruses are a sweet potato chlorotic dwarf virus, a sweet potato pinniform mottle virus and a sweet potato latent virus respectively; the detection method comprises the following steps: (1) extracting total RNA (Ribonucleic Acid) of a sample to be detected, and carrying out reverse transcription to obtain cDNA; (2) PCR amplification; (3) taking 5 mu L of the PCR amplification product in the step (2), and performing electrophoresis on 2% agarose gel containing 5 mu g/ml of ethidium olfactory (EB); (4) result judgment: PCR target amplified fragments are respectively 150bp, 280bp and 480bp; according to the invention, three kinds of sweet potato viruses can be simultaneously detected through one PCR reaction, the operation frequency is reduced, cross contamination is avoided, the usage amount of reagents, primers, templates, consumables and the like is also reduced, and time, labor and cost are saved.
Owner:HENAN UNIV OF SCI & TECH +1

Elbow type inlet passage with arc-shaped spreader plate

The invention provides an elbow type inlet passage with an arc-shaped spreader plate, and belongs to the technical field of water conservancy projects. The elbow type inlet passage comprises a linear gradually reducing section, an elbow gradually reducing section, a straight cone section and a separating pier, and is characterized in that the arc-shaped spreader plate is arranged in the elbow gradually reducing section, the longitudinal section of the arc-shaped spreader plate is in an arc shape, the central line of the arc-shaped spreader plate and the central line of the elbow gradually reducing section are consistent in shape. When water flow flows out of the linear gradually reducing section of the elbow type inlet passage, the water flow is uniform in flow speed distribution, but when the water flow flows in the elbow gradually reducing section, the mainstream of the water flow deviates to the inner side of the elbow type inlet passage and the flow speed of the inner side is higher than that of the outer side. Then, when the water flow passes through the elbow gradually reducing section of the arc-shaped spreader plate, the flowing and separating of the elbow gradually reducing section are reasonably controlled, the water flow of the inlet passage is more smooth in flowing, and the flow speed uniformity of the elbow gradually reducing section and the flow speed distribution of the outlet of the inlet passage are improved.
Owner:扬州市勘测设计研究院有限公司 +1

Primer group, kit and method for detecting pathogenic variation of magnaporthe grisea avirulent gene AvrPi9

The invention discloses a primer group, a kit and a method for detecting pathogenic variation of a magnaporthe grisea avirulence gene AvrPi9. The magnaporthe grisea avirulence gene AvrPi9 is subjected to SNP change, so that AvrPi9 gene coding is terminated in advance, and the gene is converted into a toxic gene. The primer group comprises two pairs of primers, wherein the first pair of primers is used for amplifying an AvrPi9 gene segment; and the second pair of primers is used for identifying whether SNP causing AvrPi9 to be converted into a toxic gene after mutation exists or not. The invention provides two pairs of molecular markers of magnaporthe oryzae non-toxic genes AvrPi9, a molecular detection system of AvrPi9 pathogenic variation in a magnaporthe oryzae natural population can be quickly established by utilizing the molecular markers, and the distribution of the genes in the field natural population and the dynamic change rule of the genes are understood and mastered. Therefore, rice blast disease-resistant breeding and reasonable variety layout and rotation in each region are guided, so that the breeding efficiency is improved, rice blast is more effectively controlled, and a new strategy for comprehensively preventing and treating rice blast is designed.
Owner:CHINA NAT RICE RES INST
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