Bacillus amyloliquefaciens B-1619 strain and application in preventing and controlling soil-borne disease of nightshade vegetables thereof
A technology of Bacillus amyloliquefaciens, B-1619, applied in the field of Bacillus amyloliquefaciens B-1619 and its application in the prevention and control of soil-borne diseases of Solanaceae vegetables, to achieve no pollution to the environment, safety for humans and animals, and good control effects Effect
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Embodiment 1
[0026] Isolation and preservation of Bacillus amyloliquefaciens B-1619 strain
[0027] In December 2008, the B-1619 strain was isolated by gradient dilution method in the tomato greenhouse in Shuyang facility in Suqian area. (Gradient dilution separation method: collect soil from the tomato greenhouse in Shuyang facility, weigh 10g and add it to a triangular flask filled with 90mL sterilized water, shake and cultivate for 30min (150r / min), and make a 1:10 (soil: water) suspension, and then take 1mL of the suspension and place it in 9mL sterile water to make 10 -2 Dilute the suspension, and so on, to make 10 -5 , 10 -6 Dilute the suspension; draw 200 μL each, add it to the YPGA medium plate, spread it evenly with a coating bar, and repeat each treatment 3 times. Place the above petri dishes in a constant temperature incubator at 28°C for 1-2 days. Select a single colony and transfer it to the YPGA plate for culture. After the colony grows, use an inoculation loop to streak ...
Embodiment 2
[0031] Preparation of Bacillus amyloliquefaciens B-1619 Biocontrol Bacteria Liquid
[0032] 1. Preparation of Bacillus amyloliquefaciens B-1619 biocontrol strain
[0033] Strain activation: transplant the freeze-dried and preserved B-1619 strain to YPGA medium, 28 o C for 48 hours.
[0034] Preparation of YPGA medium: Weigh 5.0g of yeast extract, 5.0g of tryptone; 5.0g of glucose, 17.0g of agar; put it into 1000ml of water, heat and mix well, then adjust the pH to 6.8-7.2, and then package it in In the test tube, the filling capacity is 1 / 5-1 / 6 of the test tube, and the test tube mouth is plugged with a stopper, 121 o C. Wet heat sterilization for 30 minutes, take out the test tube while it is hot and set it on a slope until it cools down for later use.
[0035] Preparation of fermented strains: Take a ring of activated B-1619 bacteria and transfer them into 300ml YPG culture solution packed in a 1000ml Erlenmeyer flask, at 150rpm, 28 o After cultivating at C for 48 ho...
Embodiment 3
[0046] Preparation of Bacillus amyloliquefaciens B-1619 biocontrol bacteria powder
[0047] The bacterial content that embodiment 2 obtains through fermentation is 10 10 Cfu / mL biocontrol bacteria solution was centrifuged, the supernatant was discarded, the precipitate was taken out and calcium bicarbonate was adsorbed, fully stirred and mixed, and passed through a jet mill to make the biocontrol bacteria powder uniform and form Bacillus amyloliquefaciens B-1619 bio-control bacteria powder; wherein, the centrifugation conditions are: 7000r / min, 10 minutes, the weight ratio of bio-control bacteria liquid and calcium bicarbonate is 1:2.
[0048] The bacterial content of the biocontrol bacteria powder is at least 4×10 9 cfu / mL.
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