111 results about "Potato family" patented technology

The invention relates to a bacillus amyloliquefaciens B-1619 strain and application in preventing and controlling soil-borne disease of nightshade vegetables thereof. The preservation number of the strain is CGMCC No. 4671. When the strain is applied, biocontrol strain powder is mixed with soil before seeding, and soil with biocontrol strain is covered on seeds after seeding; the biocontrol strain powder is firstly planted on the surface of the soil before seed separation; diluted biocontrol strain liquid is aligned to seeds of nightshade vegetables to be planted before planting, and the diluted biocontrol strain liquid is irrigated to the roots of the plants of nightshade vegetables during the planting; the diluted biocontrol strain liquid is irrigated to the roots of the plants of nightshade vegetables periodically after the planting; or, the application ways in each period from the seeding to the harvesting period of the nightshade vegetables are combined.

The invention discloses a lytic bacteriophage FQ44, which is classified and named as a lauraceae bacteriophage, and is preserved in the China Center for Type Culture Collection on September 29, 2020, and the preservation number of the bacteriophage is CCTCC (China Center for Type Culture Collection) NO: M2020560. The invention also discloses an application of the bacteriophage FQ44 in prevention and control of tobacco soil-borne bacterial wilt, and the bacteriophage is applied into soil by adopting a root irrigation method. According to the invention, the ralstonia solanacearum obligate bacteriophage is used for inhibiting tobacco soil-borne bacterial wilt, so that damage to common microbial communities in soil is avoided while the soil-borne bacterial wilt is prevented and controlled. Indoor related experiments show that the bacteriophage FQ44 belongs to muscular tail bacteriophage, can effectively inhibit the growth of part of tobacco ralstonia solanacearum, and has the best effect when being applied according to the optimal infection complex number (MOI 1-10); and field experiment results show that the bacterial wilt can be effectively inhibited, and the bacterial wilt inhibitor has a wide prospect.

The invention discloses bacillus velezensis LJBV19 separated from rhizosphere soil of grapes. The strain is collected in China General Microbiological Culture Collection Center of China Committee for Culture Collection of Microorganisms on April 2, 2021 and has an accession number of CGMCC No. 21804. The invention further discloses a broad-spectrum disease-preventing growth-promoting bacterium agent and application thereof in prevention and treatment of plant diseases caused by phytopathogens. The bacillus velezensis LJBV19 disclosed by the invention has broader-spectrum disease preventing and growth promoting effects and can be used for effectively controlling the plant diseases caused by paddy pathogens, grape pathogens, Solanaceae crop pathogens and wood pathogens, and the average inhibiting rate of the bacillus velezensis LJBV19 to Magnaporthe oryzae reaches up to 75.75%; and the bacillus velezensis LJBV19 can also be used for promoting growth of plants, improving crop yield, improving fruit quality, improving resistance of crops, developing agricultural economy and pushing agricultural development.

The invention relates to the field of genetic engineering and provides construction methods and applications of two PVX (potato virus X) over-expression vectors and one BiFC (bimolecular fluorescence complementation) vector. A genome of a PVX 1985 isolate is cloned to a downstream 35S promoter through genetic recombination, obtained infectious clone can infect solanaceae crops such as tobacco, tomatoes, potatoes and the like through agrobacterium infiltration. Infectious clone is reconstructed, and the over-expression vectors capable of effectively expressing one or two types of heterologous protein in a host plant body as well as the BiFC vector capable of identifying interactions between protein are obtained.

Solanum viarum is an alkaloid producing plant of the family Solanaceae with varied therapeutic uses. In order to grow the plant in large areas; one needs to have an efficient system of vegetative multiplication, which ensures its genetic uniformity, and true to the type nature. In nature largely seeds propagate plant, which could be result of cross-pollination which may result in genetic drift. The present invention provides an efficient micropropagation system, with high level of multiplication at relatively low cost of production. The multiplication ratio was as high as 1:6 and almost 95% the plants were viable and successfully cultivated in field. The present invention provides an ideal way of mass cultivation of the selected elite plant material.

The invention provides a set of production technology for preventing ralstonia solanacearum from growing and reproducing in solanaceae crops to increase crop yield by aiming at the defects of the prior art for controlling the damages of the ralstonia solanacearum to the crops. Brevibacillus choshinensis X23 has a wide bacteriostatic spectrum and good environmental safety and has antagonism effects to various pathogenic fungi, such as phytophthora parasitica var nicotianae, pyricularia grisea, alternaria alternate, phytophthora infestans and the like, so that the brevibacillus choshinensis X23 has favorable development prospect and application potential.

The invention discloses a compound microbial agent and application thereof in preventing and treating various plant diseases. The compound microbial agent has a remarkable inhibition effect on variousmain soil-borne diseases such as bacterial wilt, root rot, soft rot, black shank, gibberellic disease and root black rot caused by bacteria, fungi and oomycetes; meanwhile, the biomass of solanaceaecrop tomatoes, brassicaceous crop brassica campestris and cucurbitaceae crop cucumbers is remarkably improved, and a remarkable inhibiting effect is also achieved on tomato yellow leaf curl viruses which are plant virus diseases. The compound microbial agent effectively solves the problems of environmental pollution and food quality caused by the fact that the conventional microbial agent only aims at single pathogenic bacteria and chemical pesticide prevention and treatment, and has a good application prospect in green and environment-friendly and agricultural sustainable development.

The invention relates to a bacillus bacterium agent for nightshade crops as well as a production method and application thereof. The bacterium agent comprises bacillus licheniformis D2, bacillus subtilis B5 and bacillus amyloliquefaciens J4. The optimal proportion and optimal fermentation production of compound bacteria is realized through slant culture, first-grade seeds, second-grade seeds and mixed culture and fermentation; the living bacteria count of the compound bacterium agent is more than 3*10<10>cfu/mL. The bacillus preparation provided by the invention is used for preventing and treating soil-born diseases of the nightshade crops and the acting effect is obvious.

The invention discloses a cutting device for grafting solanaceae vegetables, and relates to the technical field of vegetable planting auxiliary equipment. The cutting device comprises a base and cutters, wherein supports are arranged on the upper surface of the base; a lifting box is arranged between the supports on the left and right sides; the lifting box is fixedly connected with the base through a connecting rod; a screw is arranged in the lifting box, and cutting platforms are arranged above and below the lifting box; supporting bases are symmetrically installed on the left side and the right side of the upper surface of each cutting platform; fastening lead screws are installed in the supporting bases; arc-shaped clamps are fixedly connected to the tail ends of the sides, close to each other, of the fastening lead screws; second driving motors are arranged between the supports; a gear is fixedly connected to the tail end of an output shaft of each second driving motor; tooth-shaped plates are arranged above the gears; the tail ends of the sides, close to each other, of the tooth-shaped plates are connected with the cutters; the cutting platforms are pushed to move up and downin the vertical direction through lifting rods and hinge bases, the cutting height of stems and branches in a solanaceae vegetable cultivation pot is adjusted, the tooth-shaped plates push the cutters to move inwards for cutting, the cutting efficiency is higher, and the cutters cannot hurt personnel.

The invention belongs to the technical field of gene engineering, particularly relates to a potato U6 RNA polymerase III type promoter, more particularly relates to StU64-1 and StU68-1 gene promoters, and further discloses a cloning method and application thereof. Two potato RNA polymerase III type promoters StU64-1 and StU68-1 are obtained by cloning in potatoes, have efficient transcriptional activity and can drive downstream sgRNA expression, and the activities of the two promoters and the feasibility of applying the promoters to potato CRISPR/Cas9 gene editing are respectively verified by virtue of an instantaneous transformation system of nicotiana benthamiana leaves and a stable transformation system of potatoes, and the CRISPR/Cas9 guided potato genome editing is realized. In the technical field of transgenosis, the promoters are not only suitable for potatoes, but also can be applied to other solanaceae crops such as tobacco.

The invention discloses a spice containing solanaceae plant secondary metabolites, a preparation method thereof and a spice product. The spice containing solanaceae plant secondary metabolites comprises the following raw materials: solanaceae plant secondary metabolites, wherein the secondary metabolites are nitrogen-containing compounds, and the boiling point of the secondary metabolites is 200-300 DEG C; andcomplex compounds of the secondary metabolites, wherein the complex compounds are obtained by reaction of the secondary metabolites and carboxyl-containing weak acids. According to the technical scheme, the harm of spices to users is reduced while the sense of smoking the spices by the users is guaranteed.

The invention discloses bacillus amyloliquefaciens with broad-spectrum antagonism, a microbial agent and application of the bacillus amyloliquefaciens, the bacillus amyloliquefaciens is named as bacillus amyloliquefaciens F012 and is preserved in China General Microbiological Culture Collection Center on September 7, 2021, and the preservation registration number is CGMCC (China General Microbiological Culture Collection Center) No.23371. The strain not only can efficiently prevent and treat tobacco bacterial wilt, but also can be applied to prevention and treatment of various plant diseases based on the broad-spectrum antibacterial effect on various pathogenic bacteria, such as tomato gray mold, tobacco root black rot, grape anthracnose, broad bean fusarium wilt, tobacco brown spot and the like; and the tobacco biomass can be obviously improved, the plant growth is promoted, the use of chemical pesticides and fertilizers is reduced, and the application prospect in the aspects of disease control and production of solanaceae crops is wide.

The invention discloses Pseudomonas sp.. The strain has a serial number of RBW-1, is collected by a Guangdong Microbial Culture Collection Center, which is located at fifth floor of experiment building, yard 100#, middle Xianlie road, Yuexiu district, Guangzhou, Guangdong, on October 9, 2020 and has an accession number of GDMCC 61209. The Pseudomonas sp. can be used for effectively preventing andcontrolling bacterial wilt of solanaceae crops, and compared with plants not employing a RBW-1 biocontrol strain, the disease incidence of the bacterial wilt of plants employing a RBW-1 biocontrol strain is lowered by 50%.

The invention provides a tree tomato as well as a cultivation method, a rapid propagation method and application thereof. The cultivation method of the tree tomato comprises the following steps of taking wild solanum wrightii as a female parent and wild solanum torvum as a male parent, and sequentially carrying out embryo rescue and acclimatization and transplantation after cross pollination to obtain the tree tomato. According to the tree tomato as well as the cultivation method, the rapid propagation method and the application thereof, the wild solanum wrightii is specifically selected as the female parent, the wild solanum torvum serves as the male parent, and the tree tomato variety obtained through hybridization is high in growth speed, developed in trunk, high in growth vigor, perennial, high in photosynthesis, high in nutrient and moisture absorption capacity, high in scion affinity, high in scion joint healing speed of stocks, good in healing, good in resistance, and capable ofresisting soil-borne diseases such as fusarium wilt, verticillium wilt, bacterial wilt and root-knot nematode disease; the rapid propagation method of the tree tomato is high in operability, high inefficiency and easy to popularize in practical application; and the tree tomato is wide in application, can be used as a stock for grafting solanaceae plants such as eggplants, tomatoes, potatoes andtobaccos, can be used as a landscape tree and is used for southern greening planting.

The invention discloses pseudomonas fluorescens pf27, an application thereof in promoting plant growth and a microbial agent pf27 prepared from the pseudomonas fluorescens pf27. Experiments prove that the microbial agent pf27 can remarkably increase the plant height, the root length and the like of plants after being treated and has a certain growth promoting function on plants; comparison between crops treated with the microbial agent pf27 with a control group indicates that the growth promoting effect of the microbial agent pf27 is higher than 21.55% and can be up to 829.14% to the maximum extent, the microbial agent pf27 has a broad-spectrum and efficient plant growth promoting function and especially can promote growth of solanaceous vegetable type crops and improve the quality of the solanaceous vegetable type crops. The problems that chemical fertilizers and other methods are poor in effect or have residues and the like are solved, agricultural sustainable development is promoted, and pseudomonas fluorescens pf27 and the microbial agent pf27 have better application prospects.

The invention relates to a microorganism compound preparation, belonging to the technical field of microorganisms. The microorganism compound preparation is prepared from a compound bacterial agent, as a medical component, formed by compounding a fermentation culture solution of preparation (a)-bacillus subtilis with the strain preservation number of CGMCC No.5652, a fermentation culture solution of preparation (b)-bacilus cereus with the strain preservation number of ATCC11077 and a fermentation culture solution of preparation (c)-bacillus licheniformis with the strain preservation number of ATCC11946, and a pharmaceutically acceptable additive as an auxiliary; and the bacterial colony total quantity ratio in the prepared microorganism compound preparation is that the bacillus subtilis with the strain preservation number of CGMCC No.5652 to the bacillus licheniformis with the strain preservation number of ATCC11946 to the bacillus licheniformis with the strain preservation number of ATCC11946 is (40-180) to (3-15) to (9-33). The microorganism compound preparation can be used for effectively preventing and treating a plurality of soil-borne diseases of solanaceae plants and has the advantage of prominent prevention effect.