Bacillus velezensis LJBV19 and application thereof

A technology of LJBV19 and Bacillus, applied in the field of microorganisms, can solve the problems of soil microbial richness and soil pH, quality and yield, and inability to obtain fertilizers, etc., to improve fruit quality, develop agricultural economy, and promote agricultural development.

Active Publication Date: 2021-07-23
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Frequent use of chemical pesticides in large quantities may easily cause environmental pollution and pesticide residue problems, and also easily lead to drug resistance of pathogenic bacteria
The long-term excessive use of chemical fertilizers leads to the reduction of soil microbial richness and soil pH, making crops unable to obtain these fertilizers, thereby reducing crop quality and yield, and directly or indirectly causing environmental pollution

Method used

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  • Bacillus velezensis LJBV19 and application thereof
  • Bacillus velezensis LJBV19 and application thereof
  • Bacillus velezensis LJBV19 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

preparation example Construction

[0033] (1) Preparation of test medium

[0034] 1) PDA medium: 200g of potatoes, 20g of glucose, 15g of agar powder, 1000mL of distilled water, sterilized by high pressure steam at 121°C for 20min.

[0035]2) LB liquid medium: tryptone 10g, yeast extract 5g, sodium chloride 10g, distilled water 1000mL, adjust pH to 7.0, sterilize by high pressure steam at 121°C for 20min. For LB solid medium, add 15 g of agar powder per liter of distilled water.

[0036] 3) Citrate medium: NaCl 5.0g, MgSO 4 ·7H 2 O 0.2g, NH 4 h 2 PO 4 1.0g, K 2 HPO 4 1.0g, sodium citrate 2.0g, 0.04% phenol red 10mL, agar 15g, distilled water 1000mL.

[0037] 4) Starch agar medium: beef extract 2.0g, egg white 17.0g, agar 15.0g, soluble starch 2.0g, distilled water 1000mL.

[0038] 5) Gelatin medium: 5.0 g of peptone, 120.0 g of gelatin, 3.0 g of beef extract, and 1000 mL of distilled water.

[0039] (2) Cultivation of test plant pathogenic bacteria strains

[0040] M. oryzae, C. viniferum, C. diplo...

Embodiment 1

[0041] Embodiment 1: Isolation and screening of bacterial strain

[0042] (1) Isolation of strains

[0043] The soil was taken from the rhizosphere of grapes, and the sampling requirement was within 20cm from the surface, because the roots of grapes are mainly distributed in this area. Soak the soil with physiological saline (m:v=1:10), oscillate evenly, and let it stand for 30 minutes. After the soil settles, take the supernatant and warm it at 80°C for 20 minutes. After warming, the supernatant was diluted 1, 10, 100, and 1000 times, spread on LB solid medium, cultured at 37°C for 1 day, and selected milky white single plaque with biofilm to be preserved. The 16S rRNA gene was amplified with 27F / 1492R primers, sequenced, compared, and a single colony belonging to Bacillus was retained. A single colony was inoculated in LB liquid medium, and cultured on a shaker at 37°C and 180 rpm for 24 hours to obtain a bacterial suspension. The bacterial suspension was mixed with 50% s...

Embodiment 2

[0047] Embodiment 2: Identification of bacterial strain LJBV19

[0048] (1) Physiological and biochemical characteristics of strain LJBV19

[0049] Observation of colony morphology: Pick an appropriate amount of lawn that has been cultured for 24 hours and inoculate it in LB liquid medium, and shake it on a shaker at 37°C and 180 rpm for 48 hours. Take 3 μL and drop it on the plate, and mainly record the colony shape, size, edge, surface, bulge shape, transparency, and color.

[0050] Gram staining: Pick an appropriate amount of bacterial lawn cultured for 24 hours and spread it evenly on a clean glass slide with a drop of distilled water in the center, air-dry, fix the smear several times over the flame, and stain with crystal violet for 1 min; After rinsing with running water, add iodine solution dropwise for 1 min; after rinsing with running water, decolorize with 95% alcohol for 30 s; after rinsing with running water, counterstain with safranin for 2-3 min; Gram-positive...

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Abstract

The invention discloses bacillus velezensis LJBV19 separated from rhizosphere soil of grapes. The strain is collected in China General Microbiological Culture Collection Center of China Committee for Culture Collection of Microorganisms on April 2, 2021 and has an accession number of CGMCC No. 21804. The invention further discloses a broad-spectrum disease-preventing growth-promoting bacterium agent and application thereof in prevention and treatment of plant diseases caused by phytopathogens. The bacillus velezensis LJBV19 disclosed by the invention has broader-spectrum disease preventing and growth promoting effects and can be used for effectively controlling the plant diseases caused by paddy pathogens, grape pathogens, Solanaceae crop pathogens and wood pathogens, and the average inhibiting rate of the bacillus velezensis LJBV19 to Magnaporthe oryzae reaches up to 75.75%; and the bacillus velezensis LJBV19 can also be used for promoting growth of plants, improving crop yield, improving fruit quality, improving resistance of crops, developing agricultural economy and pushing agricultural development.

Description

technical field [0001] The invention relates to the technical field of microbes, in particular to a Bacillus Velez LJBV19 and its application. Background technique [0002] In order to increase the income and yield of crops, chemical pesticides and chemical fertilizers have been widely used in agricultural production. The frequent and large use of chemical pesticides can easily cause environmental pollution and pesticide residue problems, and also easily lead to drug resistance of pathogenic bacteria. The long-term excessive use of chemical fertilizers leads to the reduction of soil microbial richness and soil pH, making crops unable to obtain these fertilizers, thereby reducing crop quality and yield, and directly or indirectly causing environmental pollution. Therefore, it is particularly important to develop bio-fertilizers and bio-pesticides that are environmentally friendly, improve soil fertility, enhance plant resistance, and have multiple control mechanisms. [000...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/22A01P3/00A01P21/00C12R1/07
CPCA01N63/22
Inventor 傅佩宁卢江汪博彭行吴伟彭亚纯
Owner SHANGHAI JIAO TONG UNIV
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