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Bacillus amyloliquefaciens with broad-spectrum antagonism, microbial agent and application of bacillus amyloliquefaciens

A technology of amylolytic spores and microbial agents, applied in the field of microorganisms, can solve the problems of few resources of excellent strains, narrow antibacterial spectrum, and limited application range, so as to reduce the use of chemical pesticides and fertilizers, increase the biomass of tobacco, and widely The effect of applying the foreground

Active Publication Date: 2021-12-07
QINGDAO AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] As a substitute for chemical fertilizers and pesticides, microbial agents have become a research hotspot for plant disease researchers in recent years. Although there have been a lot of research at home and abroad, currently available There are few resources of excellent strains, and there are insurmountable problems such as the strains are easy to degenerate and the effect is unstable.
Moreover, currently there are few biocontrol bacteria that can be applied to the control of tobacco bacterial wilt, and there are narrow antibacterial spectrum, unstable effect, and limited application range.

Method used

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  • Bacillus amyloliquefaciens with broad-spectrum antagonism, microbial agent and application of bacillus amyloliquefaciens
  • Bacillus amyloliquefaciens with broad-spectrum antagonism, microbial agent and application of bacillus amyloliquefaciens
  • Bacillus amyloliquefaciens with broad-spectrum antagonism, microbial agent and application of bacillus amyloliquefaciens

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Embodiment 1 Identification of antagonistic bacteria F012 strain

[0047] A broad-spectrum antagonistic strain F012 was isolated from the root soil of healthy plants in the tomato gray mold disease-affected area of ​​Qingdao City, Shandong Province in the early stage of the laboratory, and was kept in the laboratory. The morphological identification, physiological and biochemical identification and molecular biological identification of the antagonistic bacteria F012 were confirmed to be Bacillus amyloliquefaciens.

[0048] 1. Morphological Identification

[0049] like figure 1 As shown, the colony of the antagonistic bacteria F012 on the LB solid medium is irregular and circular as a whole; the initial stage is colorless and transparent, the surface is smooth, moist, sticky, and the edges are neat; There are folds on the edge, and the diameter is 2-3mm.

[0050] 2. Physiological and biochemical identification

[0051] Referring to the "Common Bacteria System Identi...

Embodiment 2

[0066] The functional qualitative identification of embodiment 2 antagonistic bacteria F012

[0067] 1. Experimental method:

[0068] Before the experiment in this example, the antagonistic bacteria F012 was cultured in shake flasks, activated and multiplied, and NA solid medium was prepared, and a plate for sterilized bacteria was prepared. After the culture medium was sterilized, wait until the temperature dropped to about 55 degrees Celsius and add 1ml of activated bacterium. The antagonistic bacteria F012 was poured onto the plate, and after solidification, it was placed in an incubator at 28°C for 24 hours. Ralstia solanacearum is carried out as above-mentioned steps and is poured out plate, after coagulation, to culture medium (the mixture of culture medium and bacterium) that is full of antagonistic bacteria F012 and the non-cultured R. solanacearum with punching device The bacteria-carrying medium of Yeerichia was punched separately. Pick out the bacterium cake (diam...

Embodiment 3

[0074] The antagonism of embodiment 3 antagonistic bacteria F012 to multiple strains of pathogenic fungi

[0075] 1. Experimental method:

[0076] Put the sterilized PDA medium in shift. After solidification, place two Oxford cups on both sides of the plate at a distance of 2 cm from the center of the plate, and then introduce the PDA medium again. After solidification, remove the Oxford cups and inoculate the pathogenic fungi in the center of the plate. Add 2 μL of antagonistic bacteria F012 to the wells on both sides. The control plate was not added with bacterial solution. After culturing at 28 degrees for 7 days, the diameter of the inhibition zone was measured, and the antibacterial rate of the antagonistic bacteria F012 against various pathogenic bacteria was calculated according to the following formula.

[0077] Bacteriostatic rate calculation formula: bacteriostatic rate=[(control colony diameter-bacteria cake diameter)-(treatment colony diameter-bacteria cake diame...

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Abstract

The invention discloses bacillus amyloliquefaciens with broad-spectrum antagonism, a microbial agent and application of the bacillus amyloliquefaciens, the bacillus amyloliquefaciens is named as bacillus amyloliquefaciens F012 and is preserved in China General Microbiological Culture Collection Center on September 7, 2021, and the preservation registration number is CGMCC (China General Microbiological Culture Collection Center) No.23371. The strain not only can efficiently prevent and treat tobacco bacterial wilt, but also can be applied to prevention and treatment of various plant diseases based on the broad-spectrum antibacterial effect on various pathogenic bacteria, such as tomato gray mold, tobacco root black rot, grape anthracnose, broad bean fusarium wilt, tobacco brown spot and the like; and the tobacco biomass can be obviously improved, the plant growth is promoted, the use of chemical pesticides and fertilizers is reduced, and the application prospect in the aspects of disease control and production of solanaceae crops is wide.

Description

technical field [0001] The invention relates to the field of microorganisms, in particular to a broad-spectrum antagonistic bacillus amyloliquefaciens, its microbial agent and its application. Background technique [0002] my country is the world's largest producer of tobacco leaves. Tobacco is an important economic crop in my country and has high requirements for the quality and safety of tobacco leaves. However, with the continuous increase of tobacco planting area, the increase of continuous cropping tobacco fields, the extension of continuous cropping years, the changes of farming system and tobacco varieties, the types of tobacco diseases are increasing, and the harm is also increasing. Among them, due to the destruction of soil structure and nutrition, tobacco bacterial wilt has become one of the main diseases of tobacco. In traditional agricultural production, the main way to prevent and control tobacco bacterial wilt is chemical control. Chemical pesticides such as ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/22A01P1/00A01P3/00A01P21/00C12R1/07
CPCC12N1/20A01N63/22
Inventor 刘新李雅华刘知祥叶青赵方贵刘广超田也
Owner QINGDAO AGRI UNIV
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