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PS mixture of compound biocontrol fungicide for preventing soil-borne diseases of vegetables

A technology for soil-borne diseases and biocontrol agents, applied in the directions of biocides, fungicides, biocides, etc., can solve the problems of chemical pesticide pollution, lack of high-efficiency biocontrol agents, and high use costs, and achieves a Conducive to export, improved control effect and cost saving effect

Active Publication Date: 2009-12-09
NANJING AGRICULTURAL UNIVERSITY
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AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to develop a biocontrol agent for the current situation that the chemical pesticides used in the prevention and control of plant soil-borne diseases are seriously polluted and the cost of use is too high, and there is no biocontrol bacterial preparation with high control effect on the mixed occurrence of various soil-borne diseases of vegetables. A compound bacterial agent for the prevention and control of various soil-borne diseases of vegetables, specially used for the prevention and treatment of vegetable bacterial wilt, blight, root-knot nematode, etc. The bacterial agent has high control efficiency and has the function of increasing production

Method used

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  • PS mixture of compound biocontrol fungicide for preventing soil-borne diseases of vegetables
  • PS mixture of compound biocontrol fungicide for preventing soil-borne diseases of vegetables
  • PS mixture of compound biocontrol fungicide for preventing soil-borne diseases of vegetables

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Embodiment Construction

[0022] Preparation of PS composite bacterial agent

[0023] A compound bacterial agent for preventing and treating soil-borne diseases of vegetables, which consists of bacterial strains PX35 and SM21, both of which are of the genus Bacillus spp., both of which are preserved in the General Microorganism Center of China Microbiological Culture Collection Management Committee. The preservation number of the PX35 strain is CGMCC NO.1930, and the preservation date is January 26, 2007; the preservation number of the SM21 strain is CGMCC NO.2058, and the preservation date is May 24, 2007.

[0024] The bacterial agent is obtained by a conventional bacterial culture method, shaken with PDA culture solution at 30°C and 180rpm for 36 hours, and then centrifuged at 4000g to obtain 13-16g wet bacteria per 1000ml culture solution, and each gram of wet bacteria contains 4×10 10 -10 15 bacteria.

[0025] Take the wet bacteria and the thalline preservation solution at a ratio of 1:40 (g / ml) ...

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Abstract

The invention provides a PS mixture of compound biocontrol fungicide for preventing the soil-borne diseases of vegetables, belonging to the technical field of crop disease prevention and cure. The PS mixture consists of two biocontrol bacteria, namely PX35 and SM21 respectively. The PS mixture is prepared through the following steps: culturing the two bacteria by shaking at 180 rpm at the temperature of 37 DEG C in a PDA (potato dextrose agar) culture solution for 20 to 24 hours respectively; then, centrifugating at 4,000g for 10min; and preparing the PS mixture from humidin and thallus preservative fluid by a ratio of 1:40 and further evenly mixing by a ratio of 1:1, wherein, the total concentration of viable bacteria in the finished product is 1*10 to 1*10 cfu / ml. Under the greenhouse condition, the control effect of the PS mixture on the bacterial wilt of solanaceae crops is 67.40%, the control effect thereof on the plague of solanaceae crops is over 77%, the control effect thereof on vegetable root knot nematode is 58.59%, and the effect of growth promotion is obvious; under the field condition, the control effect is stable, the yield can be increased by 50% to 80%; and at the same time, the PS mixture can prevent the soil from hardening.

Description

1. Technical field [0001] The invention relates to a compound biocontrol agent for preventing and treating soil-borne diseases of vegetables, belongs to the technical field of disease prevention and treatment of crops, and is specially used for the pollution-free prevention and treatment of various soil-borne diseases of vegetables. 2. Background technology [0002] The pathogens of plant soil-borne diseases are mostly soil-inhabiting bacteria, such as Ralstonia solanacearum, which causes bacterial wilt of Solanaceae crops, and Fusarium oxysporium, which causes a variety of plant blight and Verticillium wilt. Verticillium dahlaei, and the root-knot nematode Meloidogyne spp., which has become more and more harmful in various places in recent years. Bacterial wilt of Solanaceae crops is a worldwide disease, pathogenic bacteria can infect more than 50 families and more than 200 kinds of plants, causing economic losses of up to 12 billion US dollars worldwide; Loss: root-knot n...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/00A01P3/00A01P1/00A01P5/00C12N1/20C05F11/08C12R1/07A01N63/22
CPCA01N63/00A01N63/22A01N2300/00
Inventor 郭坚华薛庆云徐刘平李师默
Owner NANJING AGRICULTURAL UNIVERSITY
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