Biological organic fertilizer containing bacillus subtilis and application thereof

A technology of Bacillus subtilis and bio-organic fertilizer, applied in the field of prevention and control of root-knot nematode diseases of Solanaceae crops, can solve the problems of ineffective control and stability of control, etc., and achieve good control effect

Active Publication Date: 2015-06-10
安徽粤智徽源生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the influence of complex soil ecological factors on the bacterial strains used for root-knot nematode control, the stability of its control cannot be guaranteed, so the control effect is not obvious
And because the types of root-knot nematodes infecting different crops are different, targeted antagonistic strains are needed; and currently there is no antagonistic bacteria for the control of root-knot nematode diseases in Solanaceae crops and bio-organic fertilizers as the main component

Method used

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  • Biological organic fertilizer containing bacillus subtilis and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] A biological organic fertilizer containing Bacillus subtilis, which is made of 2 kg of Bacillus subtilis secondary culture broth, 20 kg of bean dregs, 40 kg of peat, 10 kg of chicken manure, 3 kg of wood chips, and 25 kg of straw.

[0018] The bio-organic fertilizer is prepared in the following way:

[0019] (1) Weigh 20g of agar, 10g of peptone, 5g of yeast extract, and 10g of NaCl, dissolve them in water, and add water to make the volume to 1000 mL, and adjust the pH to 7. Put the above mixture at 120 o C high temperature and high pressure sterilization for 30 minutes, cooling, laying the plate, and solidification for later use.

[0020] (2) Resuscitate Bacillus subtilis on the solid medium mentioned above, 37 o C, stand still and cultivate for 24 hours.

[0021] (3) Weigh 4g soybean cake flour, 3g corn flour, Na 2 HPO 4 0.8g, (NH 4 ) 2 SO 4 0.4g and NH 4 Cl 0.15g, dissolve in water, and add water to 1000 mL, adjust the pH to 7. Put the above mixture at 120 o C high temper...

Embodiment 2

[0026] Example 2 Bio-organic fertilizer for testing and identification

[0027] The bio-organic fertilizer prepared according to the formula of Example 1 was tested with the traditional organic fertilizer under the same conditions, and the content of each component of the two fertilizers was compared.

[0028] Table 1 Comparison of test results of bio-organic fertilizer containing Bacillus subtilis and traditional organic fertilizer

[0029]

[0030] It can be seen from Table 1 that the nitrogen content and total nutrients of the bio-organic fertilizer containing Bacillus subtilis of the present invention are higher than those of traditional organic fertilizers, and are within the range of plant absorption, and other components also meet the national organic fertilizer standards.

Embodiment 3

[0031] Example 3 Experiment on the control of tomato root-knot nematode with this biological organic fertilizer

[0032] This experiment was completed in a greenhouse, and the tested variety was Wanfen 2. The test area is 1.8 mu, with 3 treatment groups, each treatment group is 0.2 mu, and each treatment group is repeated 3 times. 3% carbofuran granules and 3.2% abamectin were used as controls. The root-knot nematodes on the tomato roots were investigated after the tomatoes were picked. The results showed that the incidence of 3% carbofuran treatment group was 82.2%, and the disease index was 0.457; the incidence of 3.2% abamectin treatment group was 73.3%, and the disease index was 0.422.

[0033] Using the combined prevention method of the present invention

[0034] a. Use the bio-organic fertilizer prepared in Example 1 as the base fertilizer, and apply 35g of organic fertilizer to each tomato plant when transplanting; After planting, water 30mL of the root of each tomato pl...

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Abstract

The invention discloses a biological organic fertilizer containing bacillus subtilis and application thereof. The organic fertilizer is particularly suitable for preventing and treating root-knot nematode diseases of nightshade crops, and is prepared from the following components in percentage by weight: 2-3 percent of a bacillus subtilis bacterium solution, 15-25 percent of bean dregs, 35-45 percent of peat, 8-12 percent of animal waste, 2-4 percent of saw dust and 20-30 percent of straws, preferably 2 percent of a bacillus subtilis bacterium solution, 20 percent of bean dregs, 40 percent of peat, 10 percent of chicken manure, 3 percent of saw dust and 25 percent of straws. The biological organic fertilizer is prepared by the following steps: uniformly mixing the raw materials, adding water which is 10 percent of total volume, stacking and fermenting; turning once every 5 days, and fermenting for 30 days to prepare the biological organic fertilizer. The biological organic fertilizer can be used as a base fertilizer during transplanting, the bacillus subtilis bacterium solution is diluted for 10000 times and is irrigated and applied to leaves and roots after transplanting, and the biological organic fertilizer can be used for effectively preventing and treating root-knot nematode diseases of nightshade crops.

Description

technical field [0001] The invention belongs to the technical field of biological pesticides, and relates to a biological organic fertilizer containing Bacillus subtilis, which is especially suitable for preventing and controlling root-knot nematode diseases of Solanaceae crops. technical background [0002] Root-knot nematode is an important disease that harms crops. After the nematode invades the root system of the plant, it will produce irregular nodules of different sizes on the fine and thick roots of the plant, that is, root knots. After the nematode parasitizes the root system of the plant, its function is destroyed, and the aboveground part of the plant grows weak and turns yellow, thereby affecting the crop yield. Similarly, root-knot nematode diseases of Solanaceae crops mainly occur on the roots of crops. Fat, swollen, deformed, tumor-like nodules are produced on the fibrous roots or lateral roots, often in the shape of candied haws; the root knots are white at ...

Claims

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Application Information

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IPC IPC(8): C05G3/02A01C21/00C05G3/60
Inventor 高宇
Owner 安徽粤智徽源生物科技有限公司
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