Pseudomonas sp. and application thereof in prevention and control on bacterial wilt of solanaceae crops
A technology for Pseudomonas and bacterial wilt, applied in the field of microorganisms, can solve problems such as danger of edible crops and environmental pollution
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Embodiment 1
[0016] Embodiment 1, screening and identification of RBW-1 strain
[0017] 1) Isolation of antagonistic strains
[0018] In tomato continuous cropping fields where bacterial wilt and other diseases are serious, look for a small number of healthy plants that survive in a small area of the diseased field without any symptoms of disease, and collect the root tissue and rhizosphere soil of healthy plants; Cut the roots into pieces and grind them thoroughly, transfer them together with the rhizosphere soil into a centrifuge tube containing 40mL 20% glycerol, shake and mix well, then dilute with a density gradient, and draw 100 microliters of 1×10 3 C FU / mL, 1×10 4 CFU / mL and 1×10 5 The suspension under the CFU / mL density gradient is spread on LB solid medium, and each density gradient is set for 3 times, and placed in a constant temperature incubator at 28°C for 48 hours. The number of colonies is about 40-60 per dish, which is suitable for strains A total of 186 strains were ...
Embodiment 2
[0031] Embodiment 2, RBW-1 control effect identification
[0032] 1) Cultivation of Pseudomonas RBW-1: inoculate RBW-1 strain in LB liquid medium at 28°C, shake at 200 rpm for 48 hours, and control the concentration of the cultured bacteria solution at OD 600 ≈1.0, get RBW-1 biocontrol bacteria suspension, set aside.
[0033] 2) Cultivation of R. solanacearum EP1: inoculate R. solanacearum EP1 in TTC liquid medium for 36 hours at 28°C and 200rpm shaking, then centrifuge to discard the supernatant, add sterile water to adjust the cell concentration of R. solanacearum EP1 to OD 600 ≈1.0, to obtain Ralstonia solanacearum EP1 bacterial suspension, set aside.
[0034] 3) Preparation of sterile soil: Take soil about 30 cm below the surface from a field that has never been planted with Solanaceae crops, and then sterilize the soil twice with high-pressure steam for future use to avoid interference from other microorganisms.
[0035] 4) Ralstonia solanacearum control test: select 9...
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