Pseudomonas sp. and application thereof in prevention and control on bacterial wilt of solanaceae crops

A technology for Pseudomonas and bacterial wilt, applied in the field of microorganisms, can solve problems such as danger of edible crops and environmental pollution

Pending Publication Date: 2021-03-19
GUIZHOU TOBACCO SCI RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chemical fungicides are quick and effective in inhibiting R. solanacearum, but there is a certain danger in the face of edible crops, and it is easy to cause environmental pollution

Method used

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  • Pseudomonas sp. and application thereof in prevention and control on bacterial wilt of solanaceae crops
  • Pseudomonas sp. and application thereof in prevention and control on bacterial wilt of solanaceae crops
  • Pseudomonas sp. and application thereof in prevention and control on bacterial wilt of solanaceae crops

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Embodiment 1, screening and identification of RBW-1 strain

[0017] 1) Isolation of antagonistic strains

[0018] In tomato continuous cropping fields where bacterial wilt and other diseases are serious, look for a small number of healthy plants that survive in a small area of ​​the diseased field without any symptoms of disease, and collect the root tissue and rhizosphere soil of healthy plants; Cut the roots into pieces and grind them thoroughly, transfer them together with the rhizosphere soil into a centrifuge tube containing 40mL 20% glycerol, shake and mix well, then dilute with a density gradient, and draw 100 microliters of 1×10 3 C FU / mL, 1×10 4 CFU / mL and 1×10 5 The suspension under the CFU / mL density gradient is spread on LB solid medium, and each density gradient is set for 3 times, and placed in a constant temperature incubator at 28°C for 48 hours. The number of colonies is about 40-60 per dish, which is suitable for strains A total of 186 strains were ...

Embodiment 2

[0031] Embodiment 2, RBW-1 control effect identification

[0032] 1) Cultivation of Pseudomonas RBW-1: inoculate RBW-1 strain in LB liquid medium at 28°C, shake at 200 rpm for 48 hours, and control the concentration of the cultured bacteria solution at OD 600 ≈1.0, get RBW-1 biocontrol bacteria suspension, set aside.

[0033] 2) Cultivation of R. solanacearum EP1: inoculate R. solanacearum EP1 in TTC liquid medium for 36 hours at 28°C and 200rpm shaking, then centrifuge to discard the supernatant, add sterile water to adjust the cell concentration of R. solanacearum EP1 to OD 600 ≈1.0, to obtain Ralstonia solanacearum EP1 bacterial suspension, set aside.

[0034] 3) Preparation of sterile soil: Take soil about 30 cm below the surface from a field that has never been planted with Solanaceae crops, and then sterilize the soil twice with high-pressure steam for future use to avoid interference from other microorganisms.

[0035] 4) Ralstonia solanacearum control test: select 9...

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Abstract

The invention discloses Pseudomonas sp.. The strain has a serial number of RBW-1, is collected by a Guangdong Microbial Culture Collection Center, which is located at fifth floor of experiment building, yard 100#, middle Xianlie road, Yuexiu district, Guangzhou, Guangdong, on October 9, 2020 and has an accession number of GDMCC 61209. The Pseudomonas sp. can be used for effectively preventing andcontrolling bacterial wilt of solanaceae crops, and compared with plants not employing a RBW-1 biocontrol strain, the disease incidence of the bacterial wilt of plants employing a RBW-1 biocontrol strain is lowered by 50%.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a pseudomonas and its application in the prevention and control of bacterial wilt of solanaceous crops. Background technique [0002] Bacterial wilt, also known as bacterial wilt, is the main disease of solanaceous crops such as tomato, eggplant, pepper, potato and tobacco. The main symptom is that the plants wilt and die quickly, and the stems and leaves remain green; It is a high temperature and humid environment, short crop rotation cycle or weak variety resistance, etc., the root system of the infected plant with bacterial wilt first browns, and soon begins to rot and disappear; cut the diseased stem near the surface, the vascular bundles are slightly browned, and White turbid sap is secreted from this part, and the browning part of the diseased stem is squeezed by hand, and milky white bacteria liquid is discharged. [0003] At present, the main method of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/27A01P1/00C12R1/38
CPCA01N63/27
Inventor 蔡刘体余知和汪汉成李鹏向立刚
Owner GUIZHOU TOBACCO SCI RES INST
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