Effective antagonistic strain for preventing and controlling continuous cropping tobacco bacterial wilt disease, microorganism organic fertilizer including effective antagonistic strain, and production method and application of effective antagonistic strain

A technology of tobacco bacterial wilt and organic fertilizer, which is applied in the field of intensive agricultural production, can solve the problems of chemical control agents damaging the environment, and the effect of tobacco bacterial wilt control is not obvious, and achieve good disinfection effect, increase colonization ability, improve The effect of soil microflora

Inactive Publication Date: 2015-04-22
NANJING AGRICULTURAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the problems in the prior art that the control effect of tobacco bacterial wilt is not obvious, and the chemical control agent destroys the environment, and provides a kind of microbial organic fertilizer using highly efficient antagonistic bacteria SQR-7, made of it, combined with The comprehensive method of soil pretreatment, the control efficiency reaches more than 80%, ensuring the sustainable development of tobacco planting industry

Method used

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  • Effective antagonistic strain for preventing and controlling continuous cropping tobacco bacterial wilt disease, microorganism organic fertilizer including effective antagonistic strain, and production method and application of effective antagonistic strain
  • Effective antagonistic strain for preventing and controlling continuous cropping tobacco bacterial wilt disease, microorganism organic fertilizer including effective antagonistic strain, and production method and application of effective antagonistic strain
  • Effective antagonistic strain for preventing and controlling continuous cropping tobacco bacterial wilt disease, microorganism organic fertilizer including effective antagonistic strain, and production method and application of effective antagonistic strain

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Experimental program
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Effect test

Embodiment 1

[0043] Production of Microbial Organic Fertilizer for Prevention and Control of Tobacco Bacterial Wilt in Continuous Cropping

[0044] (1) Isolation and identification of strains

[0045] Tobacco plants and rhizosphere soil that had experienced continuous cropping obstacles and severe bacterial wilt symptoms were collected, and the samples were stored at low temperature. The pathogenic bacteria --- Ralsotnia solanacearum (Ralsotnia solanacearum) were isolated using Kelman's TTC Agar selective medium. Then, R. solanaceae was used as indicator bacteria to collect the rhizosphere soil of healthy plants in continuous cropping tobacco bacterial wilt diseased fields to isolate antagonistic bacteria. Finally, through the pot test, the high-efficiency antagonistic strain SQR-7 was finally screened out, and the strain was stored at -70°C in 30% glycerol.

[0046] The main biological characteristics of strain SQR-7 are: Gram stain positive G + , the cell shape is rod-shaped, with motili...

Embodiment 2

[0055] Control Experiment of Tobacco Bacterial Wilt

[0056] (1) Soil pretreatment method

[0057] Use 325kg / ha of ammonium bicarbonate (=67.4kg N) and 750kg / ha of quicklime, spread evenly on the continuous cropping soil surface, water thoroughly, attach agricultural film, and cover with soil around to prevent air leakage. After 10 days of pretreatment of the soil, the film was uncovered, the soil was plowed, and the soil was aired.

[0058] (2) The use of microbial organic fertilizer

[0059] After the soil pretreatment, when the tobacco seedlings are transplanted, the above-mentioned microbial organic fertilizer is applied by hole application in an amount of 100 g per tobacco seedling.

[0060] (3) Pot experiment

[0061] The soil used for the test was tobacco continuous cropping soil, and the bacterial wilt disease was serious. Tobacco is "Safflower Dajinyuan" which is sensitive to bacterial wilt. Divided into 5 processes, as follows:

[0062] 1) T1: The local tobacco...

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Abstract

The invention belongs to the technical field of agricultural intensive production, and provides an effective antagonistic strain SQR-7 capable of comprehensively preventing and controlling a continuous cropping tobacco bacterial wilt disease, microorganism organic fertilizer manufactured by the effective antagonistic strain, and the application of the effective antagonistic strain to the continuous cropping tobacco bacterial wilt disease prevention and control. The SQR-7 strain belongs to bacillus amyloliquefaciens and is collected at the common microorganism center of the China Committee for Culture Collection of Microorganisms on April 13th in 2013, and the strain collection number is CGMCC NO. 7497. The control rate of the microorganism organic fertilizer including the effective antagonistic strain to organisms with the tobacco bacterial wilt disease reaches 80%. Soil chemical amendments are combined to conduct pretreatment on soil, the continuous cropping soil is sterilized, the number of pathogenic bacteria is lowered, soil microflora is improved, the growth of the pathogenic bacteria is restrained, the growth of tobaccos is also promoted, and the disease resistance of the tobaccos is greatly improved.

Description

technical field [0001] The invention belongs to the technical field of agricultural intensive production, and relates to a high-efficiency antagonistic bacteria SQR-7 for comprehensive prevention and control of continuous cropping tobacco bacterial wilt, a microbial organic fertilizer made from it, and its application in the prevention and control of continuous cropping tobacco bacterial wilt . Background technique [0002] Tobacco is the main economic crop in our country. Tobacco bacterial wilt is a bacterial soil-borne disease caused by Ralstonia solanacearum. It is one of the main diseases of tobacco and is seriously harmful. It is called "smoke cancer" and is widely distributed in tropical, Subtropical and some warm regions. In China, the disease commonly occurs in smoking areas such as Henan, Shandong, Jiangsu, Yunnan, Guangxi, Hebei, Hunan, Guangdong, Fujian, etc. When the disease is severe, the yield loss is about 80%, and it has become one of the culprits affecting...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F15/00C05F17/00A01P1/00A01B79/02C12R1/07
CPCA01B79/02C05F11/08C12N1/205C12R2001/07Y02W30/40
Inventor 沈标袁赛飞石俊雄施文刘艳霞杨兴明
Owner NANJING AGRICULTURAL UNIVERSITY
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