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Screening method of green manure for preventing and controlling tobacco bacterial wilt

A technology of tobacco bacterial wilt disease and screening method, which is applied in the field of green manure screening for preventing and controlling the occurrence of tobacco bacterial wilt disease, and can solve problems such as lack of systematic research and the like

Pending Publication Date: 2022-04-29
GUIZHOU TOBACCO SCI RES INST
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As one of the main measures of soil carbon input, there is little research on the regulation of soil-borne pathogens and microbial communities by green manure overturning, and there is no systematic research on the issue of which green manure can effectively prevent and control bacterial wilt

Method used

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  • Screening method of green manure for preventing and controlling tobacco bacterial wilt
  • Screening method of green manure for preventing and controlling tobacco bacterial wilt
  • Screening method of green manure for preventing and controlling tobacco bacterial wilt

Examples

Experimental program
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Effect test

Embodiment

[0044] A kind of green manure screening method for preventing and controlling tobacco bacterial wilt according to the present invention comprises the following steps:

[0045] 1. Calculation of the inhibition rate of green manure root exudates on bacterial wilt pathogens

[0046] (1) Isolation and identification of main substances of green manure root exudates

[0047] Put the green manure seeds into a 12-hole small seedling box after being sterilized, and fill the box with sterilized quartz sand (analytically pure, 40-80 mesh). After the seeds germinate for 2-3 days, add sterilized Huo The gran nutrient solution is about 2 cm, and the amount of water is maintained by regular irrigation, and placed in a constant temperature light incubator at 28°C to cultivate until the root system of the green manure is well developed. When the different green manures grow to a suitable size, first wash off the substrate with tap water, and then rinse the roots with ultra-pure water for more...

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Abstract

The invention discloses a screening method of green manure for preventing and controlling tobacco bacterial wilt, which is characterized in that the capability of green manure for preventing and controlling tobacco bacterial wilt is calculated according to the following formula: E is the prevention and control capability of green manure, A1 is the inhibition ratio of green manure root exudates to bacterial wilt pathogens under in-vitro culture, A2 is the inhibition ratio of green manure root exudates to bacterial wilt pathogens under a'limited micro simulation cosmic system 'pot experiment, and A3 is the inhibition ratio of green manure root exudates to bacterial wilt pathogens under in-vitro culture. A3 is the influence of the green manure on the activity of the bacterial wilt soil enzyme under the potting test of the limited micro simulation cosmic system, and A4 is the influence of the green manure on the ecological diversity of the bacterial wilt soil microorganisms under the potting test of the limited micro simulation cosmic system. The green manure meeting the requirements can be screened out through the capability of preventing and controlling the tobacco bacterial wilt of the green manure, so that the bacterial wilt can be effectively prevented and controlled.

Description

technical field [0001] The invention relates to a green manure screening method for preventing and controlling the occurrence of tobacco bacterial wilt, and belongs to the technical field of green manure screening. Background technique [0002] Tobacco is one of the important economic crops in my country. The area of ​​soil for tobacco planting and the yield of tobacco leaves in China rank first in the world. Suitable tobacco planting soil is the basis for producing high-quality tobacco leaves. The climate and terrain of Guizhou Province are suitable for the production of high-quality tobacco leaves, and it has become the second largest province in tobacco leaf production in my country. The output of flue-cured tobacco accounts for about 1 / 6 of the total output of the country. The long-term excessive input of chemical fertilizers and pesticides in the process of tobacco planting and the single continuous cropping of economic crops , leading to the imbalance of the soil ecolog...

Claims

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Application Information

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IPC IPC(8): C12Q1/06C12Q1/30C12Q1/34C12Q1/42C12Q1/527C12Q1/689C12R1/01
CPCC12Q1/06C12Q1/689C12Q1/34C12Q1/42C12Q1/30C12Q1/527
Inventor 刘艳霞李想付生华张恒朱经伟徐健李寒高维常杨航
Owner GUIZHOU TOBACCO SCI RES INST
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