Method for producing biocontrol fertilizer by means of brewer's grain

A technology of brewer's grains and fertilizers, which is applied in the field of high-efficiency microbial biocontrol fertilizers, can solve the problems of complicated solid medium components, increased consumption of food resources, and does not involve the field of fertilizers, so as to facilitate direct absorption by plants, reduce food safety problems, Improve the effect of soil micro-ecological environment

Inactive Publication Date: 2016-09-21
QILU UNIV OF TECH
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Problems solved by technology

[0005] In the above-mentioned patents and papers, a large number of edible agricultural products such as rice, wheat bran, and corn flour have been used, which has increased the consumption of food resources; the solid medium has complicated components and high cost; all use Beauveria bassiana spore powder as The final product requires complicated operation steps to separate and recover the spore powder, and the original solid medium is mostly discarded again; and none of them involve the field of fertilizers for plant growth promotion

Method used

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  • Method for producing biocontrol fertilizer by means of brewer's grain
  • Method for producing biocontrol fertilizer by means of brewer's grain

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Embodiment 1

[0026] 1. Seed preparation: Select Beauveria bassiana as the biocontrol fungal seed strain, and you can choose the commercially available Beauveria bassiana from Jiangxi Tianren Ecological Co., Ltd., and Xiamen Duoduo Biotechnology Co., Ltd. The company's "Duo Duo Biological" Beauveria bassiana and other bacterial agents. Insert Beauveria bassiana into SADY liquid medium (glucose 4g, peptone 1g, yeast extract powder 1g, dilute to 100mL with water, after high temperature sterilization, cool to room temperature, standby), the inoculum size is 3%; Cultivate in a shaker at 26° C. and 150 r / min for 4 days to obtain a seed solution for future use.

[0027] 2. Semi-solid fermentation: Add semi-solid medium into the fermenter, the composition ratio is: 100g of fresh brewer's grains, 20g of glucose, dilute to 1L with water, heat and boil for 30min, sterilize the semi-solid medium, wait for After cooling to room temperature, inoculate the semi-solid medium with 3% seed solution, and cu...

Embodiment 2

[0032] 1. Seed preparation: Select Beauveria bassiana as the biocontrol fungal seed strain, and you can choose the commercially available Beauveria bassiana from Jiangxi Tianren Ecological Co., Ltd., and Xiamen Duoduo Biotechnology Co., Ltd. The company's "Duo Duo Biological" Beauveria bassiana and other bacterial agents. Insert Beauveria bassiana into SADY liquid medium (glucose 4g, peptone 1g, yeast extract powder 1g, dilute to 100mL with water, after high temperature sterilization, cool to room temperature, standby), the inoculum size is 2%, in Cultivate in a shaker at 25°C and 160r / min for 5 days to obtain a seed solution for future use.

[0033] 2. Semi-solid fermentation: add semi-solid medium into the fermenter, the composition ratio is: 150g of fresh brewer's grains, 10g of glucose, dilute to 1L with water, heat and boil for 20min, sterilize the semi-solid medium, wait for After cooling to room temperature, inoculate 5% seed liquid of semi-solid medium, and culture in a...

Embodiment 3

[0037] 1. Seed preparation: Select Beauveria bassiana as the biocontrol fungal seed strain, and you can choose the commercially available Beauveria bassiana from Jiangxi Tianren Ecological Co., Ltd., and Xiamen Duoduo Biotechnology Co., Ltd. The company's "Duo Duo Biological" Beauveria bassiana and other bacterial agents. Insert Beauveria bassiana into SADY liquid medium (glucose 4g, peptone 1g, yeast extract powder 1g, dilute to 100mL with water, after high temperature sterilization, cool to room temperature, standby), the inoculum size is 2%, in Cultivate in a shaker at 25°C and 160r / min for 5 days to obtain a seed solution for future use.

[0038] 2. Semi-solid state fermentation: add semi-solid medium into the fermenter, and its composition ratio is: 150g of fresh brewer's grains, 10g of glucose, dilute to 1L with water, heat and boil for 20min, sterilize the semi-solid medium, After cooling to room temperature, inoculate 5% seed liquid of semi-solid medium, and cultivate...

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Abstract

The invention relates to a method for producing a fungus biocontrol fertilizer by means of brewer's grain and belongs to the technical fields of microbial pesticides and agricultural fertilizers. The biocontrol fungus strain is Beauveria bassiana. The method for producing the fungus biocontrol fertilizer mainly comprises the steps of seed preparation, semi-solid fermentation, solid fermentation and aftertreatment, wherein brewer's grain serves as the main carbon and nitrogen source and carrier during semi-solid fermentation and solid fermentation, so that the solid biocontrol fertilizer is prepared. A new approach is provided for recycling brewer's grain waste, and the produced biocontrol fertilizer can promote growth of plants and has a good effect of controlling various plant pests.

Description

technical field [0001] The invention belongs to the technical field of agricultural fertilizers, in particular to a microbial biocontrol fertilizer and a preparation method thereof, in particular to a high-efficiency microbial biocontrol fertilizer that resists insects and promotes growth. Background technique [0002] With the development of China's economy, as the world's largest alcoholic beverage, my country's beer consumption is gradually increasing. According to statistics, China's beer production reached 49.2185 million kiloliters in 2014, making it the largest beer producer in the world. According to statistics, for every ton of beer produced, about 0.25 tons of brewer's grains need to be produced. Brewer's grains are mainly beer raw material malt, rice, and hops that are filtered and squeezed after saccharification and gelatinization. The water content in brewer's grains is about 50% to 85%, and it is rich in nutrients. Among them, crude protein accounts for about...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05F5/00C05F17/00C12N1/14C12R1/645
CPCC05F5/008C05F17/50C12N1/14C05F11/00C05F11/08Y02A40/20Y02W30/40
Inventor 邱磊于卓于宪印李娇娇
Owner QILU UNIV OF TECH
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