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Bacillus bacterium agent for nightshade crops as well as production method and application thereof

A technology of bacillus and production method, which is applied in the field of spore mixed preparations to achieve obvious effects

Inactive Publication Date: 2016-09-28
黄忠诚
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Compared with the preparation of bacterial agents by solid fermentation, liquid fermentation has the advantages of low cost, high bacterial count, and simple production. However, at present, there are few researches on the mixed fermentation of antagonistic bacteria in liquid at home and abroad.

Method used

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  • Bacillus bacterium agent for nightshade crops as well as production method and application thereof
  • Bacillus bacterium agent for nightshade crops as well as production method and application thereof
  • Bacillus bacterium agent for nightshade crops as well as production method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0023] The composition of embodiment 1 bacillus bacterial agent

[0024]

Embodiment 2

[0025] The composition of embodiment 2 bacillus bacterial agent

[0026]

Embodiment 3

[0027] The production method of embodiment 3 bacillus bacterial agent

[0028] 1). Slope culture: Bacillus licheniformis D2, Bacillus subtilis B5, and Bacillus amyloliquefaciens J4 were respectively inoculated on solid medium under sterile conditions, and cultured at 37°C for 1 day;

[0029] 2). First-level seed cultivation: inoculate the bacteria cultured in step 1) into liquid medium under aseptic conditions, and culture Bacillus licheniformis D2, Bacillus subtilis B5, and Bacillus amyloliquefaciens J4 at 37°C for 1 day. The first-grade seeds were obtained, and the OD600 value of the bacterial suspension reached 3.0;

[0030] 3). Secondary seed cultivation: Inoculate the primary seeds into 100L fermenters with the volume ratio of the liquid culture medium as 10% inoculum. Under the condition of ℃, the stirring speed is 120r / min, the ventilation volume is 1:1.2, and the secondary seeds are obtained by culturing for 1 day;

[0031] 4). Mixed fermentation culture: the volume ...

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Abstract

The invention relates to a bacillus bacterium agent for nightshade crops as well as a production method and application thereof. The bacterium agent comprises bacillus licheniformis D2, bacillus subtilis B5 and bacillus amyloliquefaciens J4. The optimal proportion and optimal fermentation production of compound bacteria is realized through slant culture, first-grade seeds, second-grade seeds and mixed culture and fermentation; the living bacteria count of the compound bacterium agent is more than 3*10<10>cfu / mL. The bacillus preparation provided by the invention is used for preventing and treating soil-born diseases of the nightshade crops and the acting effect is obvious.

Description

technical field [0001] The invention relates to a spore mixture preparation, in particular to a bacillus preparation for preventing and treating soil-borne diseases of solanaceous crops. The invention also relates to the production method and application of the bacillus bacterial agent. technical background [0002] Botrytis cinerea, bacterial wilt and other soil-borne diseases are common plant diseases of Solanaceae crops. Chemical control is prone to negative effects such as drug resistance, pesticide residues and ecological imbalance. Biological control has the advantages of high efficiency, zero pollution, no drug resistance, and a combination of prevention and treatment. Among them, biological bacterial fertilizer can not only increase production, improve product quality, but also adjust and improve the composition of soil microorganisms, thereby reducing crop diseases to a certain extent. hazards. Screening effective antagonistic bacteria for the prevention of soil-...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/10C12R1/125C12R1/07
CPCC12N1/20A01N63/00
Inventor 黄忠诚
Owner 黄忠诚
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