Fast detection method for high-resistance botrytis cinerea bacterial strains of carbendazim based on LAMP (loop-mediated isothermal amplification) technology

A technology of Botrytis cinerea and carbendazim, which is applied in the field of rapid detection of carbendazim high resistance to Botrytis cinerea strains based on LAMP technology, and achieves the effects of high sensitivity, reduced drug use and strong specificity
CN104293971AActive Publication Date: 2015-01-21NANJING AGRICULTURAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
NANJING AGRICULTURAL UNIVERSITY
Publication Date
2015-01-21

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Abstract

The invention discloses a fast detection method for high-resistance botrytis cinerea bacterial strains of carbendazim. The method can be used for performing dynamic monitoring and early warning on botrytis cinerea bacteria causing gray mold of crops for the resistance groups of the carbendazol. In the method disclosed by the invention, a molecular detection technology having the advantages of fastness, convenience, strong specificity, high sensitivity and low cost is established on the basis of a loop-mediated isothermal amplification technology (LAMP). The detection method comprises the following steps: designing 2 pairs of specific primers on beta tubulin of high-resistance bacterial strains of the botrytis cinerea bacteria for the carbendazol, performing LAMP amplification, and judging whether products are the high-resistance bacterial strains of the botrytis cinerea bacteria for the carbendazol or not according to the colors of reaction products. If the LAMP amplification products appear to be azure, electrophoresis patterns present ladder-shaped strips, and the product amplification is generated, the products are identified as the high-resistance bacterial strains of the botrytis cinerea bacteria for the carbendazol; if the LAMP amplification products present violet, the electrophoresis patterns do not have strips, and the product amplification is not generated, then the products are identified as non high-resistance bacterial strains of the botrytis cinerea bacteria for the carbendazol. The method is simple, convenient, fast, and low in cost and has important actual significance in the resistance risk assessment of the gray mold of the crops and reasonable medication.
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Description

technical field

[0001] The present invention is based on loop-mediated isothermal amplification (loop-mediated isothermal amplification, LAMP) rapid molecular detection method for Botrytis cinerea to carbendazim-resistant strains, which can be used for Botrytis cinerea to carbendazim The dynamic monitoring of the development of resistant populations and the assessment of resistance risk provide an important theoretical basis for the control of resistance, epidemic early warning and rational drug use guidance of Botrytis cinerea. Background technique

[0002] Loop-mediated constant temperature amplification reaction (LAMP) is a novel constant temperature nucleic acid in vitro amplification technology invented by Japanese scholar Notomi et al. in 2000. It is widely used in gene diagnosis of diseases such as animals and plants. The principle of this technology is: using a set (4 types) of specific primers to cause a self-circulating strand displacement reaction under the action...

Claims

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