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Application of litsea cubeba extract as silage corn anti-mildew additive

A technology of silage corn and extracts, which is applied in the direction of preservation and application of food ingredients containing natural extracts and food ingredients as antimicrobials, and can solve the problems of high user operation level requirements, strong corrosion, and weak applicability , achieve good application prospects, increase protein content, and improve nutritional quality

Pending Publication Date: 2020-08-11
INNER MONGOLIA AUTONOMOUS REGION ACAD OF AGRI & ANIMAL HUSBANDRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, my country's silage mold inhibitors are mainly chemical and microbial products. Chemical antifungal agents mainly include organic acids (propionic acid, sorbic acid, benzoic acid, etc.), organic acid salts (calcium propionate, potassium sorbate, etc.) etc.), organic acid esters (dimethyl fumarate, parabens), these chemical agents have different effects on inhibiting mold, but the common problem is that these products are highly irritating, corrosive, and smelly Bad, and the residue is not easy to solve, resulting in unreliable product safety performance, and may also have toxic effects on animals to a certain extent
Microbial products mainly include lactic acid bacteria, propionic acid bacteria, etc. These products are of great significance to improve the quality of silage, but in terms of mold inhibition, due to the strict storage and use conditions of microbial agents, the effect is relatively unstable, which affects the user's operation level. It also has high requirements and relatively weak applicability in production

Method used

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  • Application of litsea cubeba extract as silage corn anti-mildew additive
  • Application of litsea cubeba extract as silage corn anti-mildew additive
  • Application of litsea cubeba extract as silage corn anti-mildew additive

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Collect the litsea cubeba fruit that is naturally dried in the shade, crush it into powder, pass it through a 40-mesh sieve, and set it aside. Take 10g of powder and wrap it into a paper bag and place it in a 500mL Erlenmeyer flask, add 250mL of ethyl acetate, soak for 16h, transfer all the contents of the Erlenmeyer flask to a Soxhlet extraction device, set the extraction temperature (105°C) and time (8h), and extract The liquid was filtered, and the solvent was distilled off under reduced pressure to obtain Litsea cubeba extract (Litsea cubeba oil), which was weighed and collected. The extraction rate of litsea cubeba extract is 3.52%. The extracted litsea cubeba extract was analyzed by GC-MS for its main components.

[0019] Gas chromatography conditions: HP-5MS chromatographic column, carrier gas is high-purity helium (99.999%), flow rate 20mL / min, temperature program; injection volume 1μL, start at 50°C, keep for 1min, rise to 260°C at 5°C / min ℃, keep 5min.

[0...

Embodiment 2

[0023] Emulsifier preparation: 20mL Tween 80, 60mL sterile distilled water, 10mL polyethylene glycol and 10mL 95% ethanol, shake well and set aside.

[0024] Preparation of Litsea Cubeba Extract Diluent: Add different volumes of Litsea Cubeba Extract obtained in Example 1 to the emulsifier prepared by the above method to prepare different concentrations of Litsea Cubeba Extract Dilution, Litsea Cubeba Extract The volume fractions of Litsea Cubeba extract in the diluted solution are 0.02%, 0.04%, 0.06%, 0.08%, 0.1%, 0.12%, 0.14%, 0.16%, 0.2%, 0.25%, 0.3%, 0.35%.

[0025] Four strains of Fusarium, Aspergillus flavus, Penicillium, and Rhizopus were isolated from silage corn, and four purified strains of Fusarium, Aspergillus flavus, Penicillium, and Rhizopus were respectively prepared at a concentration of 10 6 After cfu / mL bacterial suspension and spore suspension, respectively take 1mL on the potato-dextrose agar medium, culture at 25°C for 120h, under sterile conditions, repea...

Embodiment 3

[0031] Get silage corn raw material and be divided into 4 groups, every group 650g, wherein two groups add the Litsea cubeba extract that the embodiment 1 of different volumes obtains respectively, make the volume fraction of Litsea cubeba extract be 0.14% and 0.3% respectively, mix After uniformity, fill them in 1L silage tanks and seal them. The remaining two groups are blank addition (without any medicament) and chemical additives (propionic acid with a volume fraction of 0.15%). , after 45 days of silage fermentation, each treatment was repeated three times, and the dry matter, protein, crude fiber, acid detergent fiber and neutral detergent fiber, amino acids and fatty acids of corn silage were determined. The results are shown in Table 2: for the treatment of adding 0.3% of the extract of Arboria chinensis, the content of protein, fatty acid and amino acid increased by 0.56%, 0.86% and 3.52% respectively compared with the CK group; treatment, the content of protein, fatt...

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Abstract

The invention discloses an application of a litsea cubeba extract as a silage corn anti-mildew additive. The litsea cubeba extract is an extract obtained from litsea cubeba fruits by adopting a Soxhlet extraction method, and the litsea cubeba extract is directly mixed with silage corn. According to the application, the litsea cubeba extract is added into the silage corn, so that moulds of four mould genera of fusarium, aspergillus flavus, penicillium and rhizopus can be effectively inhibited, and the occurrence of moulds in the silage corn is effectively controlled; and the litsea cubeba extract can also significantly improve the protein content, the total amino acid content and the total fatty acid content in the silage corn and improve the nutritional quality of the silage corn, and hasa high application prospect.

Description

technical field [0001] The invention relates to the technical field of anti-mold additives, in particular to the application of litsea cubeba extract as an anti-mold additive for silage corn. Background technique [0002] Silage corn is an important forage product. In recent years, there are still potential safety hazards in the production, processing and use of silage corn, especially the problem of mold. The harm of mold pollution mainly has two aspects: one is to destroy the nutritional value of the feed, and to change the physical and chemical properties of the feed, such as causing discoloration, odor, and agglomeration, resulting in a decrease in palatability; the other is toxin-producing molds can produce Mycotoxins, and affect human health through the food chain milk, eggs, and meat. Mycotoxins mainly refer to the toxic metabolites produced by molds in the food they have contaminated. Therefore, preventing and controlling mildew in silage corn is the main way to re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L3/3499A23L3/349A23K30/15
CPCA23L3/3499A23L3/349A23K30/15A23V2002/00A23V2200/10A23V2250/21
Inventor 张福金张欣昕杨永青刘广华张尧栗艳芳连海飞
Owner INNER MONGOLIA AUTONOMOUS REGION ACAD OF AGRI & ANIMAL HUSBANDRY SCI
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