Microorganism composite zymocyte agent as well as preparation method and application thereof

A technology of compound fermentation inoculants and microorganisms, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of inconvenient collection and transportation, great impact on the quality of resource-based products, and reducing the protein content of kitchen waste. , to improve the protein content and the content of beneficial bacteria, inhibit the growth and reproduction of pathogenic microorganisms, keep fresh and have good sensory quality.

Inactive Publication Date: 2011-09-07
NINGBO UNIV
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

Food waste mainly includes rice and flour food residues, vegetables, vegetable oils, animal oils, etc. From the chemical composition, it includes starch, cellulose, protein, lipids, and inorganic salts. Its characteristics are as follows: 1. Crude protein and crude fiber High content of organic matter, such as high value of development and utilization, but perishable and produce stench; 2, high water content, inconvenient collection and transportation, improper treatment is likely to cause secondary pollution; 3, oil and salt content is higher than other domestic waste , has a great impact on the quality of resource-based products and requires a lot of proc

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  • Microorganism composite zymocyte agent as well as preparation method and application thereof
  • Microorganism composite zymocyte agent as well as preparation method and application thereof

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[0020] Example 1

[0021] The present invention is a microbial composite fermentation inoculum, the active components are Candida tropicalis (G. tropicalis) CGMCC NO.4201 and Lactococcus lactis (Lactococcus lactis) CGMCC NO.4202, Candida tropicalis bacteria liquid and Lactococcus lactis The bacterial liquid was mixed evenly at a mass ratio of 1:1. Candida tropicalis was deposited in the China Microbial Culture Collection and Management Center on September 30, 2010, with the preservation number CGMCCNO.4201, and the preservation address is Beichen, Chaoyang District, Beijing No. 3, No. 1 West Road, Institute of Microbiology, Chinese Academy of Sciences; Lactococcus lactis was deposited in the China Microbial Culture Collection and Management Center on September 30, 2010, the deposit number is CGMCC NO.4202, and the deposit address is Beijing No. 3, No. 1, Beichen West Road, Chaoyang District, Institute of Microbiology, Chinese Academy of Sciences, the preparation method is as foll...

Example Embodiment

[0029] Example 2

[0030] The present invention is a microbial composite fermentation inoculum, the active components are Candida tropicalis (G. tropicalis) CGMCC NO. 4201 and Lactococcus lactis (Lactococcus lactis) CGMCC NO. 4202, strains of Candida tropicalis and Lactococcus lactis According to the mass ratio of 0.5:1, the mixture is evenly mixed, and the preparation method is the same as that of Example 1.

[0031] In this specific embodiment, the active component of the microbial composite fermentation inoculum also includes Shewanella putrefacens, and the mass of the Shewanella putrefacens accounts for 25% of the composite fermentation inoculum, which is lactic acid bacteria and yeast. The bacteria provide the carbon source necessary for growth.

Example Embodiment

[0032] Example 3

[0033] The present invention is a microbial composite fermentation inoculum, the active components are Candida tropicalis (G. tropicalis) CGMCC NO. 4201 and Lactococcus lactis (Lactococcus lactis) CGMCC NO. 4202, strains of Candida tropicalis and Lactococcus lactis Mix uniformly in a mass ratio of 2:1, and the preparation method is the same as that of Example 1.

[0034] In this specific embodiment, the active component of the microbial composite fermentation inoculum also includes Shewanella putrefacens, and the mass of the Shewanella putrefacens accounts for 30% of the composite fermentation inoculum, which is lactic acid bacteria and yeast. The bacteria provide the carbon source necessary for growth.

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Abstract

The invention relates to a microorganism composite zymocyte agent as well as a preparation method and an application thereof. The invention is characterized in that the active components of the composite zymocyte agent are Cida tropicalis and lactococcus lactis which are mixed according to the mass ratio of (0.5-2): 1. The preparation process comprises the following steps: carrying out oblique plane strain culture on the Cida tropicalis and lactococcus lactis; then carrying out liquid seed amplification culture to obtain a Cida tropicalis liquid and a lactococcus lactis liquid; mixing the Cida tropicalis liquid and lactococcus lactis liquid according to the mass ratio of (0.5-2): 1 and inoculating the mixture into a solid amplification culture medium to obtain the composite zymocyte agent according to the inoculum size of 4-5%(w/v); and adding the composite zymocyte agent to kitchen waste for microbial fermentation. The invention has the advantages of suppressing growth of putrefying bacteria in the kitchen waste and reproduction of pathogenic microorganism, thus featuring good freshness-retaining effect and organoleptic quality while increasing protein content and beneficial flora content of the kitchen waste.

Description

technical field [0001] The invention relates to a composite fermenting bacterial agent and a preparation method thereof, in particular to a microbial composite fermenting bacterial agent for treating kitchen waste, a preparation method and application thereof. Background technique [0002] Food waste refers to food waste and food residues in households, schools, government canteens, and catering industries, and is the main component of urban domestic waste. Food waste mainly includes rice and flour food residues, vegetables, vegetable oils, animal oils, etc. From the chemical composition, it includes starch, cellulose, protein, lipids, and inorganic salts. Its characteristics are as follows: 1. Crude protein and crude fiber High content of organic matter, such as high value of development and utilization, but perishable and produce stench; 2, high water content, inconvenient collection and transportation, improper treatment is likely to cause secondary pollution; 3, oil and ...

Claims

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Application Information

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IPC IPC(8): C12N1/00C12N1/16C12N1/20B09B3/00C12R1/74C12R1/46C12R1/01
CPCY02W30/82
Inventor 吴祖芳翁佩芳杨亚文
Owner NINGBO UNIV
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