Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Multiple detection method and kit for simultaneously detecting three sweet potato viruses

A detection kit and technology for sweet potato virus, applied in microorganism-based methods, biochemical equipment and methods, recombinant DNA technology, etc., can solve the problem of not being able to detect more than two sweet potato viruses at the same time, saving costs and avoiding crossover. Pollution, the effect of reducing the number of operations

Pending Publication Date: 2022-07-29
HENAN UNIV OF SCI & TECH +1
View PDF0 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In order to overcome the problem that two or more sweet potato viruses cannot be detected simultaneously in the background technology, the present invention discloses a multiple detection method and a kit for simultaneously detecting three sweet potato viruses

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Multiple detection method and kit for simultaneously detecting three sweet potato viruses
  • Multiple detection method and kit for simultaneously detecting three sweet potato viruses
  • Multiple detection method and kit for simultaneously detecting three sweet potato viruses

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0035] The present invention can be explained in detail through the following examples, and the purpose of disclosing the present invention is to protect all technical improvements within the scope of the present invention.

[0036] combined with Figures 1 to 4 , a multiplex RT-PCR detection kit for simultaneous detection of 3 sweet potato viruses, the kit includes 6 1.5mL centrifuge tubes, respectively equipped with 2.5mM 10× reaction buffer, 1U / μL Taq enzyme 1.5 mL, upstream primers of 3 sweet potato viruses 10 μM, downstream primers of 3 sweet potato viruses 10 μM, pure water 1.5 mL, 15 μg / mL positive standard 0.5 mL; as required, the 10× reaction buffer is Tris-HCl 100 mM , KCl 500mM and dNTPs; Described positive standard substance is to utilize the CP gene full-length primers of described 3 kinds of sweet potato viruses to carry out PCR amplification respectively, the amplification product is sequenced, and the CP gene of the obtained positive sample carries out the cons...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the technical field of sweet potato virus detection, in particular to a multiple detection method and a kit for simultaneously detecting three sweet potato viruses. The kit comprises a 10 * reaction buffer solution, Taq enzyme, upstream primers of the three sweet potato viruses, downstream primers of the three sweet potato viruses and a positive standard substance. The three sweet potato viruses are a sweet potato chlorotic dwarf virus, a sweet potato pinniform mottle virus and a sweet potato latent virus respectively; the detection method comprises the following steps: (1) extracting total RNA (Ribonucleic Acid) of a sample to be detected, and carrying out reverse transcription to obtain cDNA; (2) PCR amplification; (3) taking 5 mu L of the PCR amplification product in the step (2), and performing electrophoresis on 2% agarose gel containing 5 mu g / ml of ethidium olfactory (EB); (4) result judgment: PCR target amplified fragments are respectively 150bp, 280bp and 480bp; according to the invention, three kinds of sweet potato viruses can be simultaneously detected through one PCR reaction, the operation frequency is reduced, cross contamination is avoided, the usage amount of reagents, primers, templates, consumables and the like is also reduced, and time, labor and cost are saved.

Description

technical field [0001] The invention relates to the technical field of sweet potato virus detection, in particular to a multiple detection method and a kit for simultaneously detecting three kinds of sweet potato viruses. Background technique [0002] As is known, sweet potato is a plant that utilizes tuberous roots to carry out asexual reproduction, which is extremely susceptible to virus infection in the cultivation process, and has a great impact on the yield and quality of sweet potato after virus infection; At present, mixed infection of various viruses has been detected in Guangdong, Fujian, Jiangsu, Sichuan, Beijing, Shandong and other places. It is reported that the loss caused by sweet potato virus in China is as high as 4 billion yuan every year. One of the important limiting factors for potato latent virus, SPLV), etc.; and the common situation is the compound infection of multiple sweet potato viruses, which greatly affects the improvement of sweet potato yield ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/70C12Q1/686C12N15/11C12R1/94
CPCC12Q1/701C12Q1/686C12Q2600/16C12Q2537/143C12Q2565/125
Inventor 张小梅李广录侯文邦
Owner HENAN UNIV OF SCI & TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com