Dogwood extraction and its preparation method and usage

Abstract

The invention provides a dogwood extraction and its preparation method and use thereof, wherein the preparation consists of extracting dogwood with alcohol, depression concentrating the raffinate to a finite density, collecting the deposition, decompressing and drying, obtaining extract A, passing through macroscopic adsorption resin column, removing foreign substance through washing, ethanol elution, collecting the eluent, decompressing and drying, obtaining extract B, mixing extract A and B homogeneously, thus obtaining the dogwood extract. The invention discloses its novel pharmaceutical use in preparing medicament for prevention and treatment of diabetic blood vessel pathological changes and for reducing blood sugar.

Description

technical field

[0001] The invention provides an extract of Cornus officinalis, and also relates to a preparation method of the extract and the medicinal use of the extract, belonging to the field of traditional Chinese medicine. Background technique

[0002] Diabetes mellitus is a chronic systemic and progressive endocrine and metabolic disease. Continuous hyperglycemia can lead to abnormal metabolism of some tissues and organs, and then cause their dysfunction and morphological changes. The duration of diabetes is more than 10 years, and most patients have vascular complications of varying degrees, especially microvascular complications. Diabetic microangiopathy refers to microcirculation disturbance, microvascular tumor formation and microvascular basement membrane thickening, mainly including diabetic nephropathy and diabetic retinopathy. In severe cases, it can lead to uremia or blindness, causing great physical and mental suffering to patients. Therefore, it is of gre...

Examples

Embodiment 1

[0164] 1000 g of Cornus officinalis medicinal materials were extracted three times by refluxing with ethanol, the ethanol concentration was 70%, the amount of alcohol added was 15 times the weight of the medicinal materials, the extraction time was 120 min, and the extraction temperature was 80°C. The extracts were combined, filtered and concentrated under reduced pressure. The concentrated solution was precipitated with 4 times the amount of water, and then centrifuged. The collected precipitate was dried under reduced pressure, pulverized to obtain extract A, and the centrifuged supernatant was concentrated under reduced pressure for use.

[0165] Take the non-polar macroporous adsorption resin, pack it into the column at a ratio of 1:1 by weight to the crude drug, and then pass the above-mentioned standby concentrate through the macroporous resin column at a flow rate of 1 column volume / hour. Elution with deionized water followed by gradient elution with 30% and 50% ethanol ...

Embodiment 2

[0168] 1000 g of Cornus officinalis medicinal materials were extracted twice with ethanol, the concentration of ethanol was 85%, the amount of alcohol added was 12 times the weight of the medicinal materials, the extraction time was 90 min, and the extraction temperature was 75°C. The extracts were combined, filtered and concentrated under reduced pressure. The concentrated solution was precipitated with 5 times the amount of water, and then centrifuged. The collected precipitate was dried under reduced pressure, pulverized to obtain extract A, and the centrifuged supernatant was concentrated under reduced pressure for use.

[0169]Take the medium-polarity macroporous adsorption resin and pack it into the column at a ratio of 1:2 by weight to the crude drug, and then pass the above-mentioned spare concentrate through the macroporous resin column at a flow rate of 1.5 column volumes / hour. Elution with deionized water, followed by gradient elution with 50% to 70% ethanol at a flo...

Embodiment 3

[0172] After crushing 1000 g of the cornus medicinal material, pass it through a 40-mesh sieve, soak it in 20% ethanol for 48 hours, and then extract it by percolation, the flow rate is 4-5 ml / min, and the percolation amount is 10 times the weight of the medicinal material. The extracts were combined, filtered and concentrated under reduced pressure. The concentrated solution was precipitated with 4 times the amount of water, and then centrifuged. The collected precipitate was dried under reduced pressure, pulverized to obtain extract A, and the centrifuged supernatant was concentrated under reduced pressure for use.

[0173] Take the medium-polarity macroporous adsorption resin, pack it into a column at a ratio of 1:3 by weight to the crude drug, and then pass the above-mentioned standby concentrate through the macroporous resin column at a flow rate of 1 column volume / hour. Elution with deionized water, followed by gradient elution with 30% to 70% ethanol at a flow rate of 2 ...

technical field

[0001] The invention provides an extract of Cornus officinalis, and also relates to a preparation method of the extract and the medicinal use of the extract, belonging to the field of traditional Chinese medicine. Background technique

[0002] Diabetes mellitus is a chronic systemic and progressive endocrine and metabolic disease. Continuous hyperglycemia can lead to abnormal metabolism of some tissues and organs, and then cause their dysfunction and morphological changes. The duration of diabetes is more than 10 years, and most patients have vascular complications of varying degrees, especially microvascular complications. Diabetic microangiopathy refers to microcirculation disturbance, microvascular tumor formation and microvascular basement membrane thickening, mainly including diabetic nephropathy and diabetic retinopathy. In severe cases, it can lead to uremia or blindness, causing great physical and mental suffering to patients. Therefore, it is of gre...

Examples

Embodiment 1

[0164] 1000 g of Cornus officinalis medicinal materials were extracted three times by refluxing with ethanol, the ethanol concentration was 70%, the amount of alcohol added was 15 times the weight of the medicinal materials, the extraction time was 120 min, and the extraction temperature was 80°C. The extracts were combined, filtered and concentrated under reduced pressure. The concentrated solution was precipitated with 4 times the amount of water, and then centrifuged. The collected precipitate was dried under reduced pressure, pulverized to obtain extract A, and the centrifuged supernatant was concentrated under reduced pressure for use.

[0165] Take the non-polar macroporous adsorption resin, pack it into the column at a ratio of 1:1 by weight to the crude drug, and then pass the above-mentioned standby concentrate through the macroporous resin column at a flow rate of 1 column volume / hour. Elution with deionized water followed by gradient elution with 30% and 50% ethanol ...

Embodiment 2

[0168] 1000 g of Cornus officinalis medicinal materials were extracted twice with ethanol, the concentration of ethanol was 85%, the amount of alcohol added was 12 times the weight of the medicinal materials, the extraction time was 90 min, and the extraction temperature was 75°C. The extracts were combined, filtered and concentrated under reduced pressure. The concentrated solution was precipitated with 5 times the amount of water, and then centrifuged. The collected precipitate was dried under reduced pressure, pulverized to obtain extract A, and the centrifuged supernatant was concentrated under reduced pressure for use.

[0169]Take the medium-polarity macroporous adsorption resin and pack it into the column at a ratio of 1:2 by weight to the crude drug, and then pass the above-mentioned spare concentrate through the macroporous resin column at a flow rate of 1.5 column volumes / hour. Elution with deionized water, followed by gradient elution with 50% to 70% ethanol at a flo...

Embodiment 3

[0172] After crushing 1000 g of the cornus medicinal material, pass it through a 40-mesh sieve, soak it in 20% ethanol for 48 hours, and then extract it by percolation, the flow rate is 4-5 ml / min, and the percolation amount is 10 times the weight of the medicinal material. The extracts were combined, filtered and concentrated under reduced pressure. The concentrated solution was precipitated with 4 times the amount of water, and then centrifuged. The collected precipitate was dried under reduced pressure, pulverized to obtain extract A, and the centrifuged supernatant was concentrated under reduced pressure for use.

[0173] Take the medium-polarity macroporous adsorption resin, pack it into a column at a ratio of 1:3 by weight to the crude drug, and then pass the above-mentioned standby concentrate through the macroporous resin column at a flow rate of 1 column volume / hour. Elution with deionized water, followed by gradient elution with 30% to 70% ethanol at a flow rate of 2 ...