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Red back leaf root extract, preparation method and application in the preparation of medicines for treating liver fibrosis

A technology of red-backed leaf root and anti-hepatic fibrosis, which is applied in the direction of drug combination, pharmaceutical formula, digestive system, etc., can solve problems such as retention, and achieve the effect of simple and reasonable extraction process

Active Publication Date: 2018-12-04
SOUTHERN MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, most of them stay in experimental research or clinical efficacy observation, and there is no drug with significant curative effect on the market.

Method used

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  • Red back leaf root extract, preparation method and application in the preparation of medicines for treating liver fibrosis

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Effect test

preparation example Construction

[0022] The present invention is a kind of preparation method of red back leaf root extract, and it comprises the following steps:

[0023] (1) The red-backed leaf root medicinal material is extracted with 6-12 times the amount of ethanol for 1-2 hours, extracted 1-3 times, combined with the ethanol extract, filtered and concentrated under reduced pressure. Preferably, the ethanol concentration in step (1) is 60%-90%, more preferably, the ethanol concentration is 70%-80%. The root of the red-backed leaf is the root of the dicotyledonous plant Euphorbiaceae plant Alchornea trewioides (Benth.) Muell.–Arg.

[0024] (2) Centrifuge the concentrate obtained in step (1), and concentrate the supernatant under reduced pressure for subsequent use.

[0025] (3) Put the concentrate obtained in step (2) on a medium polar or nonpolar macroporous adsorption resin column at a flow rate of 1.3-1.7 column volume / hour.

[0026] (4) After removing impurities by eluting with deionized water, elut...

Embodiment 1

[0031] The red-backed leaf root medicinal material was reflux extracted twice with ethanol, the ethanol concentration was 75%, the amount of alcohol added was 10 times the weight of the medicinal material, and the extraction time was 90 minutes. The extracts were combined, filtered and concentrated under reduced pressure, the concentrate was centrifuged, and the supernatant was concentrated under reduced pressure for later use.

[0032] Take a medium-polarity macroporous adsorption resin, pack it into a column at a ratio of 1:2 to the crude drug by weight, and then pass the above-mentioned concentrated solution through the macroporous resin column at a flow rate of 1.5 column volume / hour. Deionized water was used for elution, followed by gradient elution with 30-50% ethanol, and the elution flow rate was 2.5 column volumes / hour. The ethanol eluate was collected, dried under reduced pressure, and pulverized to obtain the root extract of the red dorsal leaf. The yield was 0.38%...

Embodiment 2

[0034] The red-backed leaf root medicinal material was reflux extracted with ethanol for 3 times, the ethanol concentration was 90%, the amount of alcohol added was 12 times of the weight of the medicinal material, and the extraction time was 120 min. The extracts were combined, filtered and concentrated under reduced pressure, the concentrate was centrifuged, and the supernatant was concentrated under reduced pressure for later use.

[0035] Take a medium-polarity macroporous adsorption resin, pack it into a column at a ratio of 1:2 to the crude drug by weight, and then pass the above-mentioned concentrated solution through the macroporous resin column at a flow rate of 1.5 column volume / hour. Deionized water was used for elution, followed by gradient elution with 30-60% ethanol, and the elution flow rate was 2.5 column volumes / hour. The ethanol eluate was collected, dried under reduced pressure, and pulverized to obtain the root extract of the red dorsal leaf. The yield was...

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Abstract

The invention discloses a red-backed Christmas bush root extract as well as a preparation method and application of the red-backed Christmas bush root extract in preparation of a medicine for treating hepatic fibrosis. The method comprises the following steps: performing alcohol extraction, centrifugal condensation, macroreticular resin column, ethanol elution and reduced pressure drying to obtain an effective part extract of the red-backed Christmas bush root. The extraction process of the red-backed Christmas bush root extract is simple and reasonable, and the obtained effective part extract of the red-backed Christmas bush root can reduce ALT and AST, as well as HA, LN and III procollagens and TGFbeta1 and TIMP-1 in serums of model animals so as to prove that the red-backed Christmas bush root extract has an ideal anti-hepatic fibrosis function, is a promising candidate medicine and can be applied to preparation of anti-hepatic fibrosis medicines.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine, and in particular relates to an extract of the effective part of the root of the red dorsal leaf, and also relates to a preparation method and application of the extract. Background technique [0002] Liver fibrosis is one of the intractable diseases that seriously endanger the physical and mental health of human beings, and no effective prevention and treatment method has been found so far. Liver fibrosis is the early process of chronic hepatitis developing to liver cirrhosis, and it plays a vital role in determining the prognosis of patients with chronic liver disease. The occurrence and development of fibrosis will produce a major breakthrough in the treatment of chronic liver diseases. In terms of modern medicine, colchicine is an anti-hepatic fibrosis drug that was first used clinically, but its clinical application is limited due to its strong toxic and side effects. Glucocorti...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/47A61P1/16
Inventor 吕志平刘强
Owner SOUTHERN MEDICAL UNIVERSITY
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