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Effective portion extraction of dogwood and its preparation method and usage

A technology of effective parts and extracts, applied in the field of traditional Chinese medicine, can solve the problem of no diabetic vascular disease, and achieve the effects of improving the morphological and pathological changes of vascular endothelium and kidney tissue, the extraction process is simple and reasonable, and the amount of production is reduced.

Inactive Publication Date: 2005-01-26
江苏中康药物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, so far, there has not been a preparation process for the effective fraction of the total iridoid glycosides of Cornus officinalis, nor has there been any report on the use of the effective fraction in the preparation of drugs for preventing and treating diabetic vascular disease and lowering blood sugar.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0156] 1000 g of Cornus officinalis medicinal material was reflux extracted with ethanol for 3 times, the ethanol concentration was 90%, the amount of alcohol added was 15 times of the weight of the medicinal material, the extraction time was 120 min, and the extraction temperature was 75°C. The extracts were combined, filtered and concentrated under reduced pressure. The concentrated solution was precipitated by adding 4 times the amount of water and then centrifuged. The supernatant was concentrated under reduced pressure for later use.

[0157] Take the non-polar macroporous adsorption resin, pack it into a column at a ratio of 1:1 to the weight ratio of the crude drug, and then pass the above-mentioned concentrated solution for use through the macroporous resin column at a flow rate of 1 column volume / hour. Deionized water was used for elution, followed by gradient elution with 30-50% ethanol, and the elution flow rate was 2 column volumes / hour. The ethanol eluate was coll...

Embodiment 2

[0159] 1000 g of Cornus officinalis medicinal material was reflux extracted twice with ethanol, the ethanol concentration was 80%, the amount of alcohol added was 12 times of the weight of the medicinal material, the extraction time was 90 min, and the extraction temperature was 80°C. The extracts were combined, filtered and concentrated under reduced pressure. The concentrated solution was precipitated by adding 5 times the amount of water and then centrifuged. The supernatant was concentrated under reduced pressure for later use.

[0160] Take a medium-polarity macroporous adsorption resin, pack it into a column at a ratio of 1:2 to the weight ratio of the crude drug, and then pass the above-mentioned standby concentrated solution through the macroporous resin column at a flow rate of 1.5 column volume / hour. After passing through completely, use Deionized water was used for elution, and then 50-70% ethanol was used for gradient elution, and the elution flow rate was 2.5 colum...

Embodiment 3

[0162] 1000g of cornus officinalis is crushed and passed through a 40-mesh sieve, impregnated with ethanol for 48 hours, and then extracted by percolation. The ethanol concentration is 20%, the flow rate is 4-5 ml / min, and the percolation amount is 10 times the weight of the medicinal material. The extracts were combined, filtered and concentrated under reduced pressure. The concentrated solution was precipitated by adding 4 times the amount of water and then centrifuged. The supernatant was concentrated under reduced pressure for later use.

[0163] Take a medium-polarity macroporous adsorption resin, pack it into a column at a ratio of 1:1 to crude drug by weight, and then pass the above-mentioned standby concentrated solution through the macroporous resin column at a flow rate of 1 column volume / hour. After passing through completely, use Deionized water was used for elution, followed by gradient elution with 30-50% ethanol, and the elution flow rate was 2 column volumes / hou...

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Abstract

The invention discloses the effective portion extraction of dogwood and its preparation method and use, wherein the preparation consists of extracting dogwood with alcohol, depression concentrating the extract liquid, charging water for sediment, purifying through macroscopic adsorption resin column, removing foreign substance through washing, ethanol elution, collecting the eluent, decompressing and drying, thus obtaining the effective position extract of dogwood. The invention discloses its novel pharmaceutical use in preparing medicament for prevention and treatment of diabetic blood vessel pathological changes and for reducing blood sugar.

Description

technical field [0001] The invention relates to an extract of effective parts of Cornus officinalis, a preparation method and application of the extract, and belongs to the field of traditional Chinese medicines. Background technique [0002] Diabetes mellitus is a chronic systemic progressive endocrine and metabolic disease. Sustained high blood sugar can lead to abnormal metabolism of some tissues and organs, and then cause their dysfunction and morphological changes. The duration of diabetes is more than 10 years, and most patients are complicated with vascular complications of varying degrees, especially microvascular complications. Diabetic microangiopathy refers to microcirculatory disturbance, microangioma formation and thickening of microvascular basement membrane, mainly including diabetic nephropathy and diabetic retinopathy, which can lead to uremia or blindness in severe cases, causing great physical and mental pain to patients. Therefore, it is of great signifi...

Claims

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Application Information

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IPC IPC(8): A61K31/7048A61P3/10A61P13/12A61P27/02C07H1/08C07H17/04
Inventor 肖伟潘扬丁岗章晨峰韩淑燕曹亮
Owner 江苏中康药物科技有限公司
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