Bacterial synthetic community and application thereof in preparation of biological control agent for controlling postharvest diseases of citrus fruits

A postharvest disease and bacteria technology, applied in microorganism-based methods, bacteria, applications, etc., can solve the problems of short duration of disease control, and achieve the effect of lasting disease control, control of occurrence and progress, and good application potential.

Pending Publication Date: 2021-04-23
SOUTHWEST UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The use of microbial agents as a biological control method to control postharvest diseases of fruits and vegetables has been extensively studied. The available nutrients are fixed, and the duration of disease control is short

Method used

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  • Bacterial synthetic community and application thereof in preparation of biological control agent for controlling postharvest diseases of citrus fruits
  • Bacterial synthetic community and application thereof in preparation of biological control agent for controlling postharvest diseases of citrus fruits
  • Bacterial synthetic community and application thereof in preparation of biological control agent for controlling postharvest diseases of citrus fruits

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Embodiment Construction

[0014] In order to make the object, technical solution and beneficial effect of the present invention clearer, preferred embodiments of the present invention will be described in detail below.

[0015] 1. Experimental method

[0016] 1. Isolation, purification and identification of bacteria

[0017] Use an electric toothbrush to clean the epiphytic bacteria on the surface of the fruit in PBS buffer solution, apply gradient dilution on potato dextrose agar medium (PDA) and meat extract peptone medium (LB), and pick a single colony after culturing at 37°C for 12-24 hours . The isolated bacteria were purified by plate streaking method, purified for two generations, cultured in liquid medium and identified by molecular biological methods. The strains were preserved using the glycerin preservation method and stored in a -80°C refrigerator.

[0018] 2. Screening of Bacterial Members of the Synthetic Community

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Abstract

The invention discloses a bacterial synthetic community, which is composed of eight bacteria, namely Acinetobacter lwoffii, Bacillus amyloliquefaciens, Curptobacterium luteum, Curptobacterium sp., Methylobacterium platani, Pantoea sp., Pseudomonas parasulva and Pseudomonas sp., compared with a single bacterial strain, the bacterial synthetic community can better control the occurrence and progress of citrus green mold and sour rot, can more enduringly control diseases, can be used for preparing a biological control agent for controlling postharvest diseases of citrus fruits, has good application potential. A new thought and method are provided for controlling the postharvest diseases of the citrus fruits, and the application range of the bacterial synthetic community is widened.

Description

technical field [0001] The invention belongs to the technical field of biological control of postharvest diseases of fruits, and relates to a bacterial synthetic community and its application in the preparation of biological control agents for controlling postharvest diseases of citrus fruits. Background technique [0002] Citrus fruits are easily infected by Penicillium digitatum and Geotrichum candidum during postharvest storage and transportation, causing green mold and acid rot diseases, resulting in a large amount of economic losses. [0003] Microorganisms have a close relationship with their hosts, widely exist in various organic life forms, and have an important impact on the health and growth of the host. The use of microbial agents as a biological control method to control postharvest diseases of fruits and vegetables has been extensively studied. The nutrients available are fixed, and the duration of disease control is short. Contents of the invention [0004]...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23B7/155C12R1/07C12R1/38C12R1/01
CPCC12N1/20A23B7/155
Inventor 曾凯芳荆佳伊王淑培易兰花邓丽莉
Owner SOUTHWEST UNIVERSITY
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