The invention belongs to the technical field of agriculture and in particular discloses a biological preparation for preventing and treating candidatus liberibacter of red honey pomelos. The biological preparation is prepared from the following raw materials in parts by weight: 20-30 parts of a traditional Chinese medicine extract, 0.1-0.2 part of a complex microbial bacterium, 0.5-1 part of fructose, 0.5-1 part of a film formation agent, 0.1-0.2 part of a stabilizer, 0.2-0.3 part of monopotassium phosphate, 0.2-0.3 part of ferrous sulfate, 0.02-0.04 part of epsilon-polylysine and 0.01-0.02 part of citric acid, wherein the traditional Chinese medicine extract is prepared from the following components in parts by weight: 6-8 parts of onions, 3-5 parts of chamomile, 10-15 parts of Chinese violet, 10-15 parts of salix leaves, 8-10 parts of herba schizonepetae, 3-5 parts of radix ophiopogonis, 3-5 parts of scutellaria baicalensis, 4-6 parts of ailanthus altissima, 8-10 parts of eucalyptusleaves, 3-5 parts of radix sophorae flavescentis and 4-6 parts of fennel. The biological preparation disclosed by the invention has a very good killing function on vector insects, namely woodlice which propagate candidatus liberibacter, and candidatus liberibacter pathogenic bacteria, namely gram negative bacteria, in addition, is good in stability, that is, a good medicinal effect can be maintained for a long time in a natural environment, and is remarkable in effect in preventing and treating candidatus liberibacter of red honey pomelos.