The invention provides a jujube growth conditioning nutrient solution for preventing and suppressing fruit shrink disease and a preparation method thereof. The nutrient solution comprises water, manganese sulfate, zinc sulfate, boron sulfate, ammonia molybdate, gibberellin and brassin lactone. The preparation method comprises the following preparation processes: adding the water into the manganesesulfate, the zinc sulfate, the boron sulfate and the ammonia molybdate, stirring together, putting into a reaction kettle after fully mixing evenly, heating, putting into a cooling tank after reacting, cooling, then putting back to a stirrer, adding the gibberellin and the brassin lactone into the stirrer sequentially and stirring evenly. The nutrient solution can provide nutrient components required by the development of jujube fruits and the cleavage of cells, condition the growth mechanism of the jujube fruits, enhance the reverse resistance of the jujube, replenish trace elements and extraneous-source hormones required by the jujube fruits, promote the continuity of the cell cleavage of the jujube fruits, strengthen the synchronous effect of various hormones, such as growth hormones,the gibberellin, cell cleavage hormones and the like, and the concentrated supply of various trace elements in the bodies of the jujube fruits and effectively control the fruit stalks of the jujube fruits to form a separation layer, thereby achieving the aims of preventing and suppressing the occurance of the fruit shrink disease of the jujube fruits.