Detection paper for simultaneously and quantitatively detecting IgG and IgM contents of novel coronavirus and method

Abstract

The invention discloses detection paper for simultaneously detecting IgG and IgM of the novel coronavirus and a method. A nitrocellulose membrane in the detection paper is provided with a first detection strip sprayed with an anti-human IgM monoclonal antibody, a second detection strip sprayed with an anti-human IgG monoclonal antibody and a quality control strip sprayed with a monoclonal antibodyof the specific protein of anti-novel coronavirus 2019-nCoV, wherein the first detection strip, the second detection strip and the quality control strip are distributed at intervals; a conjugate padis sprayed with rare earth ion nanosphere labeled specific protein of novel coronavirus 2019-nCoV. The test strip can detect the IgG and IgM contents of novel coronavirus 2019-nCoV at the same time, which provides convenience for the clinical diagnosis of novel coronavirus 2019-nCoV.

Description

technical field [0001] The invention relates to a detection paper for detecting IgG and IgM content and a detection method thereof, more specifically, relates to a detection paper and a detection method for simultaneously quantitatively detecting the IgG and IgM content of a new coronavirus, belonging to the field of in vitro detection. Background technique [0002] Since the outbreak of the novel coronavirus 2019-nCoV pneumonia, it has spread globally and seriously threatened human life and health. Symptomatic infection brings great challenges to epidemic prevention and control and clinical diagnosis. At present, nucleic acid detection and virus gene sequencing are mainly used as evidence for etiological confirmation. Due to various factors such as sampling, operation, and reagents, nucleic acid detection will produce false negative results, and it is highly suspected that the virus of patients infected with 2019 novel coronavirus (2019-nCoV) The positive detection rate of...

AI Technical Summary

Problems solved by technology

At present, nucleic acid detection and virus gene sequencing are mainly used as evidence for etiological confirmation. Due to various factors such as sampling, operation, and reagents, nucleic acid detection will produce false negative results, and it is highly suspected that the virus of patients infected with 2019 novel coronavirus (2019-nCoV) The positive detection rate of nucleic acid is only 30% to 50%. In addition, nucleic acid detection has high requirements on equipment, testing sites and environmental conditions, and has disadvantages such as long detection time and low throughput, which is not convenient for large-scale detection of people under the current epidemic situation. Therefore, there is an urgent need to develop a rapid and convenient detection kit for clinical detection, so as to isolate the infected population as soon as possible to block the spread of the virus. The "New Coronavirus Pneumonia Diagnosis and Treatment Program (Trial Seventh Edition)" issued by the National Health and Health Commission Increase serological testing: the new coronavirus-specific IgM antibody is mostly positive 3-5 days after the onset, and the IgG antibody titer in the recovery period is 4 times or more higher than that in the acute phase. The detection method of the new coronavirus-specific IgG antibody / IgM antibody There are enzyme-linked immunoassay, chemiluminescence, colloidal gold and other immunoassay methods to detect patients' venous blood, which is safer and more convenient than throat swab sampling, greatly reducing the risk of infection and virus transmission among medical staff. The method and chemiluminescence method have high sensitivity and can be used for quantitative detection, but they have high requirements for equipment, and the reaction time is generally more than 1 hour, while the colloidal gold method is easy to operate, does not require equipment, and the detection result can be obtained by visual observation within 15 minutes. However, the colloidal gold method has shortcomings such as low sensitivity and incapable of quantitative analysis. In order to quantitatively detect the new coronavirus-specific IgG antibody / IgM antibody and obtain results quickly, the present invention combines nanotechnology and immunochromatography , established a time-resolved fluorescent immunochromatographic detection method for simultaneous quantitative detection of the IgG and IgM content of the new coronavirus 2019-nCoV, and prepared the corresponding detection card. This technology can make up for the high requirements, time-consuming and low positive rate of nucleic acid detection It can also overcome the lack of colloidal gold detection sensitivity and quantitative analysis. It can be used as an important supplementary detection method for disease diagnosis, and is suitable for rapid screening of the population, which is conducive to the diagnosis, prevention and control of 2019-nCoV infection

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