Detection paper for simultaneously and quantitatively detecting IgG and IgM contents of novel coronavirus and method

A technology for quantitative detection and detection paper, which is applied to the detection paper of IgG and IgM content and its detection field, can solve the problems of reducing the risk of medical staff being infected and virus transmission, inconvenient for large-scale detection of people, and incapable of quantitative analysis, etc. The amount of sample, the convenience of detection operation, the effect of solving the quenching problem

Pending Publication Date: 2020-07-28
浙江理工大学绍兴生物医药研究院有限公司 +1
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Problems solved by technology

At present, nucleic acid detection and virus gene sequencing are mainly used as evidence for etiological confirmation. Due to various factors such as sampling, operation, and reagents, nucleic acid detection will produce false negative results, and it is highly suspected that the virus of patients infected with 2019 novel coronavirus (2019-nCoV) The positive detection rate of nucleic acid is only 30% to 50%. In addition, nucleic acid detection has high requirements on equipment, testing sites and environmental conditions, and has disadvantages such as long detection time and low throughput, which is not convenient for large-scale detection of people under the current epidemic situation. Therefore, there is an urgent need to develop a rapid and convenient detection kit for clinical detection, so as to isolate the infected population as soon as possible to block the spread of the virus. The "New Coronavirus Pneumonia Diagnosis and Treatment Program (Trial Seventh Edition)" issued by the National Health and Health Commission Increase serological testing: the new coronavirus-specific IgM antibody is mostly positive 3-5 days after the onset, and the IgG antibody titer in the recovery period is 4 times or more higher than that in the acute phase. The detection method of the new coronavirus-specific IgG antibody/IgM antibody There are enzyme-linked immunoassay, chemiluminescence, colloidal gold and other immunoassay methods to detect patients' venous blood, whic

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  • Detection paper for simultaneously and quantitatively detecting IgG and IgM contents of novel coronavirus and method
  • Detection paper for simultaneously and quantitatively detecting IgG and IgM contents of novel coronavirus and method
  • Detection paper for simultaneously and quantitatively detecting IgG and IgM contents of novel coronavirus and method

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Embodiment 1

[0031] like figure 1 and 2As shown, a detection paper for simultaneous quantitative detection of the IgG and IgM content of the new coronavirus 2019-nCoV in this embodiment includes a base plate 4 and absorbent paper that is sequentially adhered to the base plate 4 along the length direction of the base plate 4 1. A nitrocellulose membrane 2, a bonding pad 8 and a sample pad 3; wherein, the nitrocellulose membrane 2 is glued to the middle part of the bottom plate 4, and the absorbent paper 1, the nitrocellulose membrane 2, The binding pad 8 and the sample pad 3 are sequentially and only in contact with adjacent parts and partially overlapped; the nitrocellulose membrane 2 is provided with spaced first anti-human IgM monoclonal antibody spray coatings. Detection zone 7, the second detection zone 6 of anti-human IgG monoclonal antibody, and the quality control zone 5 of the monoclonal antibody sprayed with anti-new coronavirus 2019-nCoV specific protein, and the bonding pad 8 i...

Embodiment 2

[0037] like image 3 As shown, on the basis of Example 1, this example further provides a time-resolved fluorescent immunochromatographic detection card for quantitatively detecting the IgG and IgM content of the new coronavirus 2019-nCoV simultaneously, which includes the method described in Example 1. Detection paper and a casing covering the detection paper; the casing includes a base and a card cover 9, and the card cover 9 is provided with a local area observation port 10 and a sample injection port 11 for exposing the detection paper; wherein, The sample injection port 11 is arranged above the sample pad 3 to expose part or all of the sample pad 3 area, and the observation port 10 is arranged on the top of the nitrocellulose membrane 2 to expose the first detection zone 7 . The second detection belt 6 and the quality control belt 5. In this embodiment, the detection card with a casing is not only convenient for storage and carrying, but also convenient for detection oper...

Embodiment 3

[0045] This embodiment provides a method for quantitative detection of the IgG and IgM content of the new coronavirus 2019-nCoV using the paper strip described in the embodiment, comprising the following steps:

[0046] (1) Draw a standard curve:

[0047] Dilute high-concentration IgG and IgM of the new coronavirus 2019-nCoV into a series of standard products. In fact, it can be detected according to a certain gradient. The smaller the gradient value, the finer the division. Concentration is the abscissa, establish the equation and fit the standard curve.

[0048] (2) Sample testing:

[0049] Take 20ul sample (serum or plasma or whole blood) dropwise into the sample hole of the test paper, then add 50ul sample diluent (0.05mol / L containing 1% BSA, phosphate buffer solution with pH 7.2), wait Detect immediately after 15 minutes;

[0050] Use a fluorescence detector to scan the fluorescence intensity of the first detection zone 7, the second detection zone 6, and the quality ...

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Abstract

The invention discloses detection paper for simultaneously detecting IgG and IgM of the novel coronavirus and a method. A nitrocellulose membrane in the detection paper is provided with a first detection strip sprayed with an anti-human IgM monoclonal antibody, a second detection strip sprayed with an anti-human IgG monoclonal antibody and a quality control strip sprayed with a monoclonal antibodyof the specific protein of anti-novel coronavirus 2019-nCoV, wherein the first detection strip, the second detection strip and the quality control strip are distributed at intervals; a conjugate padis sprayed with rare earth ion nanosphere labeled specific protein of novel coronavirus 2019-nCoV. The test strip can detect the IgG and IgM contents of novel coronavirus 2019-nCoV at the same time, which provides convenience for the clinical diagnosis of novel coronavirus 2019-nCoV.

Description

technical field [0001] The invention relates to a detection paper for detecting IgG and IgM content and a detection method thereof, more specifically, relates to a detection paper and a detection method for simultaneously quantitatively detecting the IgG and IgM content of a new coronavirus, belonging to the field of in vitro detection. Background technique [0002] Since the outbreak of the novel coronavirus 2019-nCoV pneumonia, it has spread globally and seriously threatened human life and health. Symptomatic infection brings great challenges to epidemic prevention and control and clinical diagnosis. At present, nucleic acid detection and virus gene sequencing are mainly used as evidence for etiological confirmation. Due to various factors such as sampling, operation, and reagents, nucleic acid detection will produce false negative results, and it is highly suspected that the virus of patients infected with 2019 novel coronavirus (2019-nCoV) The positive detection rate of...

Claims

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Application Information

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IPC IPC(8): G01N33/569G01N33/558G01N33/533G01N21/64
CPCG01N33/56983G01N33/558G01N33/533G01N21/6408G01N21/6428G01N2021/6439
Inventor 黄飚吴健王毅刚周秀梅赵雪芹
Owner 浙江理工大学绍兴生物医药研究院有限公司
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