Preparation method of human immunodeficiency virus (HIV) antibody detection test paper

An immunodeficiency virus and antibody detection technology, which is applied in measurement devices, instruments, scientific instruments, etc., can solve problems such as time-consuming and laborious, and achieve the effect of accurate diagnosis results.

Inactive Publication Date: 2013-10-02
SUZHOU WANMUCHUN BIOLOGICAL TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Non-invasive testing is to analyze the collected oral fluid to obtain diagnostic results, but this method is time-consuming and laborious, requiring a long wait for results

Method used

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  • Preparation method of human immunodeficiency virus (HIV) antibody detection test paper

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Embodiment Construction

[0018] The preferred embodiments of the present invention are described in detail below, so that the advantages and features of the present invention can be more easily understood by those skilled in the art, so as to define the protection scope of the present invention more clearly.

[0019] (1) Containers and materials

[0020] Container equipment: beakers, measuring cylinders, volumetric flasks, glass rods, magnetic stirrers, electronic balances, pipette guns, film spraying equipment, cutting machines, strip cutting machines, sealing machines, shell pressing machines, pH meters, constant temperature vacuum Drying box.

[0021] Reagent accessories: chloroauric acid, trisodium citrate, KCl, K 2 CO 3 , Tris (trishydroxymethylaminomethane), HCl, BSA (bovine serum albumin), EDTA (ethylenediaminetetraacetic acid), sodium lauryl sulfate, T-20, Brij-35 (dodecyl poly glycol ethers), sucrose, trehalose, sodium azide.

[0022] Materials: glass fiber film, polyester film, NC film, ...

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Abstract

The invention discloses a preparation method of human immunodeficiency virus (HIV) antibody detection test paper. The preparation method comprises the following steps of: spraying gold on a gold cushion by a mixed solution of a P74 recombinant antigen mark and a P44 recombinant antigen mark through membrane marking and gold spraying equipment to obtain a first gold cushion; spraying gold on the gold cushion by a rabbit IgG monoclonal antibody mark to obtain a second gold cushion; marking on a polyvinyl chloride bottom plate stuck with a nitrocellulose membrane by a detection line covering solution and a control line covering solution to obtain a polyvinyl chloride bottom plate with a marked membrane; assembling a sample cushion, the first gold cushion, the second gold cushion, the polyvinyl chloride bottom plate with the marked membrane and water absorption paper; and cutting the assembly into the detection test paper. In the manner, the detection test paper prepared by the preparation method disclosed by the invention can be used for judging whether an oral cavity sample contains an HIV antibody or not based on an immune lateral flow chromatography technology through manual operation and naked eye reading; the diagnosis is rapid, the result is accurate, and damages to a body of a subject are not caused.

Description

technical field [0001] The invention relates to the technical field of biological applications, in particular to a preparation method of a human immunodeficiency virus antibody detection test paper. Background technique [0002] Human Immunodeficiency Virus (Human Immunodeficiency Virus, HIV) is a lentivirus that infects cells of the human immune system. , semen, vaginal secretions, saliva, urine, breast milk, cerebrospinal fluid and brain interstitial fluid with neurological symptoms. Human immunodeficiency virus can destroy the immune ability of the human body, causing the immune system to lose its resistance, thus causing various diseases and cancers to survive in the human body, and eventually lead to AIDS. [0003] For AIDS, early diagnosis is conducive to the treatment of patients as soon as possible, and also helps to control the spread of the disease, so early diagnosis is particularly important. At present, the detection of AIDS is divided into blood test and non-...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/531G01N33/532
Inventor 程柯
Owner SUZHOU WANMUCHUN BIOLOGICAL TECH
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