Method for tissue culture of paeonia lactiflora

A technique of tissue culture and peony, applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problem of not being able to provide a basis for production, not forming a method for rapid propagation of peony underground buds, and not involving rooting of peony tissue culture seedlings and Transplanting and other issues to achieve the effect of environmental friendliness, low cost and simple operation

Inactive Publication Date: 2011-07-20
BEIJING FORESTRY UNIVERSITY
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AI Technical Summary

Problems solved by technology

At present, the in vitro rapid propagation research of Paeoniae plants is mostly concentrated on peony, and there are few domestic and foreign researches on the tissue culture of Paeoniae. The entire set of methods for rapid propagation using the underground buds of peony does not involve the rooting and transplanting of tissue cultured seedlings of peony, and cannot provide a basis for production

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0020] 1. Materials and methods

[0021] In October, the underground buds of the 3-4-year-old peony cultivar 'Zianshengfen' in the field were taken as explants, stored in a refrigerator at 4°C for 4 weeks, and then inoculated.

[0022] Before inoculation, dilute the commercially available "Goldfish brand" detergent 800 times with water, soak and clean the explants in the diluted detergent solution, then wash with water, and then use tweezers to peel off the outer scales, leaving the innermost Remove one or two layers of yellow-white scales and cut off the excess at the base, then soak in diluted detergent solution, carefully brush the gaps between the scales and lateral buds with a brush, and finally rinse with tap water for 1 hour.

[0023] In the ultra-clean workbench, soak the buds with one or two layers of scales in 70% alcohol for 60 seconds, rinse them with sterile water for 3 times, and soak them in diluted 84 disinfectant for 15 minutes. Dilute with a volume ratio of ...

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PUM

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Abstract

The invention provides a method for tissue culture of paeonia lactiflora, which comprises the following steps of: sterilizing a paeonia lactiflora stem tip; and performing primary culture, secondary culture and rooting culture on the sterilized paeonia lactiflora stem tip, wherein the sterilization of the paeonia lactiflora stem tip is implemented by two steps to ensure that the stem tip is sterilized thoroughly, an explant is not damaged, and the survival rate of the stem tip is high. The method for tissue culture of the paeonia lactiflora is simple to operate, has a low cost and a high rooting rate, can realize transplantation out of a bottle, and is suitable for rapid propagation of most ornamental paeonia lactiflora varieties; and simultaneously, according to the culture method provided by the invention, the excellent characteristics of an original species can be kept, and a base is established for high-quality seed breeding and industrial production.

Description

technical field [0001] The invention relates to tissue culture technology, in particular to a method for culturing peony tissue. Background technique [0002] Peony (Paeonia lactiflora) is a perennial herb of the family Paeoniaceae. It is a traditional famous flower in my country and has a long history of cultivation and ornamental. The traditional propagation methods of peony include ramets, sowing, cutting and layering, etc. Among them, the ramet propagation method is the most widely used and is also conducive to maintaining the original excellent traits of the variety. However, the propagation coefficient of this method is small and cannot meet the requirements of industrial production. . With the opening of the market, the excellent ornamental characteristics of peony and the quality of cut flowers have attracted worldwide attention. The annual demand for peony in Europe and the United States is very large, but the production situation of peony seedlings in my country i...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 于晓南潘瞳张启翔
Owner BEIJING FORESTRY UNIVERSITY
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