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Construction of targeted photodynamic nanoprobes based on upconversion nanoparticles and ultrathin silica layer

A nanoprobe and nanoparticle technology, applied in the field of materials and analytical chemistry, can solve problems such as quenching, and achieve the effect of reducing potential and excellent tumor inhibition effect.

Active Publication Date: 2021-04-06
QINGDAO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the fluorescence of UCNPs with a diameter smaller than 10 nm is easily quenched by the environment.

Method used

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  • Construction of targeted photodynamic nanoprobes based on upconversion nanoparticles and ultrathin silica layer
  • Construction of targeted photodynamic nanoprobes based on upconversion nanoparticles and ultrathin silica layer
  • Construction of targeted photodynamic nanoprobes based on upconversion nanoparticles and ultrathin silica layer

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preparation example Construction

[0036] In a typical embodiment of the present invention, the UCNPs@SiO 2 The preparation method of / MB / PEG-FA nanometer probe, this method comprises:

[0037] (1)UCNPs@SiO 2 Preparation of / MB:

[0038] Mix and stir cyclohexane and surfactant to form reverse micelles, add UCNPs and mix and stir to form a mixed system 1;

[0039] Add methylene blue and ammonia solution to mixing system 1, mix and stir to form mixing system 2;

[0040] Add silicon source to the mixed system 2, mix the reaction, the partially hydrolyzed negatively charged silicon source is transferred to the hydrophilic interior of the reverse micelles to interact with the positively charged methylene blue, and UCNPs@SiO are obtained by hydrolysis and condensation 2 / MB;

[0041] (2)UCNPs@SiO 2 Preparation of / MB / PEG-FA:

[0042] The carboxylated PEG-FA was carboxyl activated, and then the activated mixture was added to the UCNPs@SiO 2 / MB, mixed reaction to prepare UCNPs@SiO 2 / MB@PEG-FA.

[0043] In on...

Embodiment 1

[0053] The NaYF of embodiment 1 oleic acid protection 4 :Yb 3+ ,Ho 3+ / Tm 3+ Preparation of UCNPs:

[0054] (1) Preparation of rare earth oleate

[0055] Weigh 1.129gY 2 o 3 (5mmol) and 20mL concentrated hydrochloric acid in a round-bottomed flask, cover with plastic wrap, pierce holes, and stir at 60°C. After reacting overnight, adjust the temperature to 140°C, evaporate concentrated hydrochloric acid to obtain YCl 3 . Synthesized YCl 3 Add 10mL of ultrapure water and ultrasonically dissolve. Then add 5mL of ultrapure water, filter it with a syringe and a 0.22um green filter, inject it into a flask, add 20mL of ethanol, 35mL of cycloethane, and 30mmol of sodium oleate, and reflux at 78°C for 4h. Then lower the temperature to 30-40°C, transfer it into a separatory funnel, and take the upper layer solution. Then add 20mL ultrapure water and 20mL ethanol (shake evenly). Take the upper layer by layer, and separate the liquid three times. Then move it into a round bot...

Embodiment 2

[0060] The NaYF of embodiment 2 oleic acid protection 4 :Yb 3+ ,Er 3+ Preparation of UCNPs:

[0061] Same as Example 1, the difference is: in the oleate of the light-emitting layer, Y:Yb:Er=80:18:2, the molar ratio, the total molar number is 5 mmol.

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Abstract

The invention discloses a targeted photodynamic nanoprobe based on upconversion nanoparticles and an ultrathin silicon dioxide layer. The nanoprobe comprises an upconversion nanoparticle core and ultrathin silicon dioxide wrapped on the surface of the core A shell, methylene blue loaded on said shell, and polyethylene glycol-folate. Constructed UCNPs@SiO 2 The / MB / PEG-FA nanoprobe is distributed in the cytoplasm of cancer cells through receptor-mediated endocytosis, and generates reactive oxygen species under the excitation of near-infrared light, which reduces the mitochondrial membrane potential in cells and induces irreversible cell regulation in cancer cells. Death. Constructed UCNPs@SiO 2 The / MB / PEG‑FA nanoprobe also exhibited excellent tumor-inhibitory effects in animal experiments.

Description

technical field [0001] The invention relates to the field of materials and analytical chemistry, in particular to a novel folic acid-polyethylene glycol-modified ultrathin silica-coated upconversion nanoparticle as a photosensitizer carrier to realize effective photodynamic therapy. Background technique [0002] As a clinically approved cancer treatment method, photodynamic therapy uses light to excite a photosensitizer (PS) to generate reactive oxygen species, which irreversibly damage tumor cells. Photodynamic therapy can control light irradiation to specific tumor sites, reducing potential toxic side effects on normal tissues. However, it still faces some challenges such as the limited penetration depth of UV-vis light, low ROS generation efficiency, short half-life and limited action range of ROS. Cells and tissues have little absorption and scattering of near-infrared light. Therefore, near-infrared light has better penetrating ability to biological tissues, and the ba...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K41/00A61K9/51A61K47/04A61K47/69A61K47/54A61P35/00
CPCA61K9/5115A61K41/0057A61K41/008A61K47/545A61K47/6929A61K47/6935A61P35/00
Inventor 王宗花岳姿宏宋昕玥
Owner QINGDAO UNIV
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