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Identification method, device, equipment and storage medium of single-cell miRNA regulatory network

A technology for regulating networks and identifying methods, applied in the field of genetic engineering

Active Publication Date: 2021-06-04
UNIV OF ELECTRONICS SCI & TECH OF CHINA
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The object of the present invention is to provide a single-cell miRNA regulatory network identification method, device, equipment and storage medium to solve the problem of constructing a single-cell-specific Problems with miRNA regulatory networks

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Embodiment Construction

[0036]In order to make the purpose, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the drawings in the embodiments of the present invention. Obviously, the described embodiments It is a part of embodiments of the present invention, but not all embodiments.

[0037] It should be noted that although miRNA is used for description in this application, those skilled in the art should be clear that miRNA is a kind of non-coding RNA (ncRNA). In some other embodiments, the single-cell miRNA regulation provided by this application The identification method, device, equipment and storage medium of the network can also be extended and applied to other non-coding RNAs, such as long-chain non-coding RNA (lncRNA), circular RNA (circRNA) and pseudogene (pseudogene), etc., to identify lncRNA, circRNA , the regulatory ne...

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Abstract

The invention provides an identification method, device, equipment and storage medium of a single-cell miRNA regulatory network, and relates to the technical field of genetic engineering. The identification method of the single-cell miRNA regulatory network comprises: obtaining single-cell transcriptome data of miRNAs and target genes of matched samples. The number of matching samples is obtained. If the number of matching samples is less than the preset threshold, a pseudo single cell is inserted into the matching samples by an interpolation method to obtain an expanded matching sample, and the matching sample is updated to the expanded matching sample. According to the statistical correlation value of miRNA and target gene, calculate the significance value of the association relationship between miRNA and target gene, and determine whether there is a regulatory relationship between miRNA and target gene in a single cell according to the significance value. The regulatory relationship between miRNAs and target genes determined from the single-cell transcriptome data of matched samples is fused to obtain a single-cell miRNA regulatory network. Realize the ability to construct the miRNA regulatory network of each single cell when not enough matching samples can be obtained.

Description

technical field [0001] The present invention relates to the technical field of genetic engineering, in particular to an identification method, device, equipment and storage medium of a single-cell miRNA regulatory network. Background technique [0002] MicroRNA (miRNA) plays an important role in many cellular biological processes such as cell cycle, cell differentiation, cell proliferation, cell growth, cell migration and cell apoptosis. Previous studies have shown that the selective expression of genes is the basis of cell specificity, and this selective expression is regulated by miRNA to a certain extent. If the selective expression is misregulated by miRNA, it will lead to complex human diseases including cancer. However, it is still insufficient to study the regulatory network between miRNA and target genes at the single-cell level. [0003] In the prior art, methods for identifying miRNA regulatory networks at the cell level are usually based on dual single-cell trans...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G16B5/00G16B25/00
CPCG16B5/00G16B25/00
Inventor 张俊鹏饶妮妮王光斌
Owner UNIV OF ELECTRONICS SCI & TECH OF CHINA
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