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A cosmid is a type of hybrid plasmid that contains a Lambda phage cos sequence . Cosmids (cos sites + plasmid = cosmids) DNA sequences are originally from the lambda phage. They are often used as a cloning vector in genetic engineering. Cosmids can be used to build genomic libraries. They were first described by Collins and Hohn in 1978. Cosmids can contain 37 to 52 (normally 45) kb of DNA, limits based on the normal bacteriophage packaging size. They can replicate as plasmids if they have a suitable origin of replication (ori): for example SV40 ori in mammalian cells, ColE1 ori for double-stranded DNA replication, or f1 ori for single-stranded DNA replication in prokaryotes. They frequently also contain a gene for selection such as antibiotic resistance, so that the transformed cells can be identified by plating on a medium containing the antibiotic. Those cells which did not take up the cosmid would be unable to grow.
A three-gene deleted bovine herpes virus 1, its construction method and application
This invention belongs to the field of viral genetic engineering technology and discloses a bovine herpesvirus type 1 (BHV-1) with three gene deletions, its construction method, and its applications. Using a Fosmid library, four myxovirus rescue combinations were screened. FosBHV-1-b-ΔTK-mCherry and FosBHV-1-d-ΔgEct / US9-eGFP were obtained through Red / ET and Gateway methods. These were then combined with FosBHV-1-a and FosBHV-1-c to construct an infectious cloning platform for recombinant BHV-1 with the TK / gEct / US9 three-gene deletions, rescuing recombinant viruses carrying tracer genes. This method has high recombination efficiency, and the cloning platform can efficiently construct BHV-1 gene-deleted strains. The immunogenicity of the deleted strains is comparable to that of wild-type viruses, with good safety, providing an efficient technical means for the development of BHV-1 attenuated vaccines and bovine disease live vector vaccines.
Owner:CHINA AGRI UNIV
Recombinant cosmid of duck adenovirus B2 reverse genetic operating system and application of recombinant cosmid
The invention relates to a recombinant cosmile of a duck adenovirus B2 reverse genetic operating system and application of the recombinant cosmile, the recombinant cosmile is a recombinant cosmile BG18FOS-2 and is constructed by using duck adenovirus B2 DAdV-B2 / delta ORF55-ORF19, the duck adenovirus B2 DAdV-B2 / delta ORF55-ORF19 is preserved in the China Center for Type Culture Collection, and the preservation number is CCTCC NO: V202568. Compared with a duck adenovirus B2 strain BGMH, the recombinant cosmid BG18FOS-2 sequence has the differences as shown in SEQ ID No.1-2, a deletion region located between a gene ORF20 and a gene ORF53 and the like. The recombinant cosmile can successfully rescue a virus with the same biological characteristics as the duck adenovirus B2 DAdV-B2 / delta ORF55-ORF19, and a reverse genetic operating system containing the recombinant cosmile is successfully constructed.
Owner:INST OF ANIMAL HUSBANDRY & VETERINARY FUJIAN ACADEMY OF AGRI SCI